CIA1/YDR267C Protein Information

Standard Name	Cia1p 1
Systematic Name	Ydr267cp
ORF Classification	Verified
Description	Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein (1, 2)
Name Description	Cytosolic Iron-sulfur protein Assembly 1

Experimental Data
Molecules/cell	5640 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	330
Molecular Weight (Da)	37,274
Isoelectric Point (pI)	4.44

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cia1p (InterPro)
Physical Interactions	There are 15 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006A234 \| Q05583 MIPS: YDR267C NCBI: 1230640 \| 151567598 \| 398366361 \| 74676366 \| NP_010553.3 GenBank/EMBL/DDBJ: DAA12111.1 \| U51030

Amino Acid Sequence (or in FASTA format)
1 MASINLIKSL KLYKEKIWSF DFSQGILATG STDRKIKLVS VKYDDFTLID 51 VLDETAHKKA IRSVAWRPHT SLLAAGSFDS TVSIWAKEES ADRTFEMDLL 101 AIIEGHENEV KGVAWSNDGY YLATCSRDKS VWIWETDESG EEYECISVLQ 151 EHSQDVKHVI WHPSEALLAS SSYDDTVRIW KDYDDDWECV AVLNGHEGTV 201 WSSDFDKTEG VFRLCSGSDD STVRVWKYMG DDEDDQQEWV CEAILPDVHK 251 RQVYNVAWGF NGLIASVGAD GVLAVYEEVD GEWKVFAKRA LCHGVYEINV 301 VKWLELNGKT ILATGGDDGI VNFWSLEKAA *

Amino Acid Sequence (or in FASTA format)

       1  MASINLIKSL KLYKEKIWSF DFSQGILATG STDRKIKLVS VKYDDFTLID
      51  VLDETAHKKA IRSVAWRPHT SLLAAGSFDS TVSIWAKEES ADRTFEMDLL
     101  AIIEGHENEV KGVAWSNDGY YLATCSRDKS VWIWETDESG EEYECISVLQ
     151  EHSQDVKHVI WHPSEALLAS SSYDDTVRIW KDYDDDWECV AVLNGHEGTV
     201  WSSDFDKTEG VFRLCSGSDD STVRVWKYMG DDEDDQQEWV CEAILPDVHK
     251  RQVYNVAWGF NGLIASVGAD GVLAVYEEVD GEWKVFAKRA LCHGVYEINV
     301  VKWLELNGKT ILATGGDDGI VNFWSLEKAA *

external links for Cia1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Cia1p

1)	Balk J, et al. (2005) The essential WD40 protein Cia1 is involved in a late step of cytosolic and nuclear iron-sulfur protein assembly. Mol Cell Biol 25(24):10833-41
2)	Stehling O, et al. (2012) MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity. Science 337(6091):195-9
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41