MET32/YDR253C Protein Information

Standard Name	Met32p
Systematic Name	Ydr253cp
ORF Classification	Verified
Description	Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; lack of such a loop for MET31 may account for the differential actions of Met32p and Met31p; MET32 has a paralog, MET31, that arose from the whole genome duplication (1, 2, 3)
Name Description	METhionine requiring 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	191
Molecular Weight (Da)	21,518
Isoelectric Point (pI)	10.67

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Met32p (InterPro)
Physical Interactions	There are 6 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004F8F8 \| Q12041 MIPS: YDR253C NCBI: 1136208 \| 1226029 \| 2501691 \| 45269349 \| 6320459 \| NP_010539.1 GenBank/EMBL/DDBJ: DAA12093.1 \| AY557729 \| Z68329 \| Z70202

Amino Acid Sequence (or in FASTA format)
1 MEDQDAAFIK QATEAIVDVS LNIDNIDPII KELLERVRNR QNRLQNKKPA 51 LIPAENGVDI NSQGGNIKVK KENALPKPPK SSKSKPQDRR NSTGEKRFKC 101 AKCSLEFSRS SDLRRHEKTH FAILPNICPQ CGKGFARKDA LKRHYDTLTC 151 RRNRTKLLTA GGEGINELLK KVKQSNIVHR QDNNHNGSSN G*

Amino Acid Sequence (or in FASTA format)

       1  MEDQDAAFIK QATEAIVDVS LNIDNIDPII KELLERVRNR QNRLQNKKPA
      51  LIPAENGVDI NSQGGNIKVK KENALPKPPK SSKSKPQDRR NSTGEKRFKC
     101  AKCSLEFSRS SDLRRHEKTH FAILPNICPQ CGKGFARKDA LKRHYDTLTC
     151  RRNRTKLLTA GGEGINELLK KVKQSNIVHR QDNNHNGSSN G*

external links for Met32p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Met32p

1)	Blaiseau PL, et al. (1997) Met31p and Met32p, two related zinc finger proteins, are involved in transcriptional regulation of yeast sulfur amino acid metabolism. Mol Cell Biol 17(7):3640-8
2)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3)	McIsaac RS, et al. (2012) Perturbation-based analysis and modeling of combinatorial regulation in the yeast sulfur assimilation pathway. Mol Biol Cell 23(15):2993-3007