VHS1/YDR247W Protein Information

Standard Name	Vhs1p 1
Systematic Name	Ydr247wp
ORF Classification	Verified
Description	Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; VHS1 has a paralog, SKS1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	Viable in a Hal3 Sit4 background 1

Experimental Data
Molecules/cell	784 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	461
Molecular Weight (Da)	52,136
Isoelectric Point (pI)	6.88

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Vhs1p (InterPro)
Physical Interactions	There are 78 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006B500 \| Q03785 MIPS: YDR247W NCBI: 50401413 \| 6320453 \| 817833 \| NP_010533.1 \| NM_001180555.1 GenBank/EMBL/DDBJ: DAA12088.1 \| Z49701
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MMMFHNCRIN NYLITSQIGE GAYGLVYRAL DIRTDRQYAI KAVVQSYGVS 51 KEADMGNDKI HKNSVKLQKK LAKLFKESKN VVRVPSIDLE SIENMSEEDF 101 KKLPHYKEIS LHLRVHHHKN IVTIHEVLQS AVCTFIVMDY YPTDLFTSIV 151 DNRHFVTNGL LVKKVFLQIC SALNYCHEHG IYHCDIKPEN LLLDTEDNVF 201 LCDFGLSTTS TYIKPNVCIG SSYYMPPERI SFDGRVSSSK SGGHKLGKVC 251 PSCNGDLWSL GIILINLTCI RNPWLKADKT EDNTYYYFTK DPNILKQILP 301 LSDDFYSLLS KILQVNPKNR MSLQELMKEV SSITSFTNEG PLSKVPPLSK 351 SVYEKFVSPV DNTNENLSPK SYVYMHDSKA AKNLSYTSSS EEEDGIKEGI 401 DDDNGSRSGS FGTLDTDTGL HSSFTSTSCE SDNECSKISN KFSLFEKKFN 451 ELRMSSSSLT N*

Amino Acid Sequence (or in FASTA format)

       1  MMMFHNCRIN NYLITSQIGE GAYGLVYRAL DIRTDRQYAI KAVVQSYGVS
      51  KEADMGNDKI HKNSVKLQKK LAKLFKESKN VVRVPSIDLE SIENMSEEDF
     101  KKLPHYKEIS LHLRVHHHKN IVTIHEVLQS AVCTFIVMDY YPTDLFTSIV
     151  DNRHFVTNGL LVKKVFLQIC SALNYCHEHG IYHCDIKPEN LLLDTEDNVF
     201  LCDFGLSTTS TYIKPNVCIG SSYYMPPERI SFDGRVSSSK SGGHKLGKVC
     251  PSCNGDLWSL GIILINLTCI RNPWLKADKT EDNTYYYFTK DPNILKQILP
     301  LSDDFYSLLS KILQVNPKNR MSLQELMKEV SSITSFTNEG PLSKVPPLSK
     351  SVYEKFVSPV DNTNENLSPK SYVYMHDSKA AKNLSYTSSS EEEDGIKEGI
     401  DDDNGSRSGS FGTLDTDTGL HSSFTSTSCE SDNECSKISN KFSLFEKKFN
     451  ELRMSSSSLT N*

external links for Vhs1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Vhs1p

1)	Munoz I, et al. (2003) Identification of multicopy suppressors of cell cycle arrest at the G1-S transition in Saccharomyces cerevisiae. Yeast 20(2):157-69
2)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3)	Zhu H, et al. (2000) Analysis of yeast protein kinases using protein chips. Nat Genet 26(3):283-9
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41