PRP28/YDR243C Protein Information

Standard Name	Prp28p 1
Systematic Name	Ydr243cp
ORF Classification	Verified
Description	RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site (2, 3)
Name Description	Pre-mRNA Processing

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	588
Molecular Weight (Da)	66,641
Isoelectric Point (pI)	9.36

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Prp28p (InterPro)
Physical Interactions	There are 10 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005321A \| P23394 MIPS: YDR243C NCBI: 1172596 \| 398366237 \| 666103 \| 817829 \| NP_010529.3 GenBank/EMBL/DDBJ: DAA12083.1 \| X56934 \| Z49701
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides]

Amino Acid Sequence (or in FASTA format)
1 MARPIDVSQL IAGINKKKGL DENTSGKISK PRFLNKQERS KQERLKENEE 51 SLTPTQSDSA KVEIKKVNSR DDSFFNETND KKRNPSKQNG SKFHFSWNES 101 EDTLSGYDPI VSTRAIDLLW KGKTPKNAAE SSYMGKHWTE KSLHEMNERD 151 WRILKEDYAI VTKGGTVENP LRNWEELNII PRDLLRVIIQ ELRFPSPTPI 201 QRITIPNVCN MKQYRDFLGV ASTGSGKTLA FVIPILIKMS RSPPRPPSLK 251 IIDGPKALIL APTRELVQQI QKETQKVTKI WSKESNYDCK VISIVGGHSL 301 EEISFSLSEG CDILVATPGR LIDSLENHLL VMKQVETLVL DEADKMIDLG 351 FEDQVTNILT KVDINADSAV NRQTLMFTAT MTPVIEKIAA GYMQKPVYAT 401 IGVETGSEPL IQQVVEYADN DEDKFKKLKP IVAKYDPPII IFINYKQTAD 451 WLAEKFQKET NMKVTILHGS KSQEQREHSL QLFRTNKVQI MIATNVAARG 501 LDIPNVSLVV NFQISKKMDD YIHRIGRTGR AANEGTAVSF VSAAEDESLI 551 RELYKYVRKH DPLNSNIFSE AVKNKYNVGK QLSNEIIY*

Amino Acid Sequence (or in FASTA format)

       1  MARPIDVSQL IAGINKKKGL DENTSGKISK PRFLNKQERS KQERLKENEE
      51  SLTPTQSDSA KVEIKKVNSR DDSFFNETND KKRNPSKQNG SKFHFSWNES
     101  EDTLSGYDPI VSTRAIDLLW KGKTPKNAAE SSYMGKHWTE KSLHEMNERD
     151  WRILKEDYAI VTKGGTVENP LRNWEELNII PRDLLRVIIQ ELRFPSPTPI
     201  QRITIPNVCN MKQYRDFLGV ASTGSGKTLA FVIPILIKMS RSPPRPPSLK
     251  IIDGPKALIL APTRELVQQI QKETQKVTKI WSKESNYDCK VISIVGGHSL
     301  EEISFSLSEG CDILVATPGR LIDSLENHLL VMKQVETLVL DEADKMIDLG
     351  FEDQVTNILT KVDINADSAV NRQTLMFTAT MTPVIEKIAA GYMQKPVYAT
     401  IGVETGSEPL IQQVVEYADN DEDKFKKLKP IVAKYDPPII IFINYKQTAD
     451  WLAEKFQKET NMKVTILHGS KSQEQREHSL QLFRTNKVQI MIATNVAARG
     501  LDIPNVSLVV NFQISKKMDD YIHRIGRTGR AANEGTAVSF VSAAEDESLI
     551  RELYKYVRKH DPLNSNIFSE AVKNKYNVGK QLSNEIIY*

external links for Prp28p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Prp28p

1)	Strauss EJ and Guthrie C (1991) A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family. Genes Dev 5(4):629-41
2)	Staley JP and Guthrie C (1999) An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p. Mol Cell 3(1):55-64
3)	de la Cruz J, et al. (1999) Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci 24(5):192-8