SIR4/YDR227W Protein Information

Standard Name	Sir4p 1
Systematic Name	Ydr227wp
Alias	Asd1p , Ste9p , Uth2p
ORF Classification	Verified
Description	Silent information regulator that, together with SIR2 and SIR3, is involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; potentially phosphorylated by Cdc28p; some alleles of SIR4 prolong lifespan (2, 3, 4)
Name Description	Silent Information Regulator 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,358
Molecular Weight (Da)	152,060
Isoelectric Point (pI)	9.77

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sir4p (InterPro)
Physical Interactions	There are 167 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052F71 \| P11978 MIPS: YDR227W NCBI: 134508 \| 33357530 \| 433286893 \| 433286894 \| 49258491 \| 49258492 \| 531116 \| 532751 \| 6320433 \| 728683 \| NP_010513.1 GenBank/EMBL/DDBJ: DAA12069.1 \| M37249 \| U13239 \| Z48612

Amino Acid Sequence (or in FASTA format)
1 MPNDNKTPNR SSTPKFTKKP VTPNDKIPER EEKSNEVKTP KIPLFTFAKS 51 KNYSRPSTAI HTSPHQPSDV KPTSHKQLQQ PKSSPLKKNN YNSFPHSNLE 101 KISNSKLLSL LRSKTSAGRI ESNNPSHDAS RSLASFEQTA FSRHAQQQTS 151 TFNSKPVRTI VPISTSQTNN SFLSGVKSLL SEEKIRDYSK EILGINLANE 201 QPVLEKPLKK GSADIGASVI SLTKDKSIRK DTVEEKKEEK LNIGKNFAHS 251 DSLSVPKVSA GDSGISPEES KARSPGIAKP NAIQTEVYGI NEESTNERLE 301 INQEKPVKLD ENSANSTVAS ALDTNGTSAT TETLTSKKIV PSPKKVAIDQ 351 DKITLHDEKT LAPSKHQPIT SEQKMKEDAD LKRMEILKSP HLSKSPADRP 401 QGRRNSRNFS TRDEETTKLA FLVEYEGQEN NYNSTSRSTE KKNDMNTSAK 451 NKNGENKKIG KRPPEIMSTE AHVNKVTEET TKQIQSVRID GRKVLQKVQG 501 ESHIDSRNNT LNVTPSKRPQ LGEIPNPMKK HKPNEGRTPN ISNGTINIQK 551 KLEPKEIVRD ILHTKESSNE AKKTIQNPLN KSQNTALPST HKVTQKKDIK 601 IGTNDLFQVE SAPKISSEID RENVKSKDEP VSKAVESKSL LNLFSNVLKA 651 PFIKSESKPF SSDALSKEKA NFLETIASTE KPENKTDKVS LSQPVSASKH 701 EYSDNFPVSL SQPSKKSFAN HTEDEQIEKK KICRGRMNTI ITHPGKMELV 751 YVSDSDDSSS DNDSLTDLES LSSGESNEIK VTNDLDTSAE KDQIQAGKWF 801 DPVLDWRKSD RELTKNILWR IADKTTYDKE TITDLIEQGI PKHSYLSGNP 851 LTSVTNDICS VENYETSSAF FYQQVHKKDR LQYLPLYAVS TFENTNNTEK 901 NDVTNKNINI GKHSQEQNSS SAKPSQIPTV SSPLGFEETK LSTTPTKSNR 951 RVSHSDTNSS KPKNTKENLS KSSWRQEWLA NLKLISVSLV DEFPSELSDS 1001 DRQIINEKMQ LLKDIFANNL KSAISNNFRE SDIIILKGEI EDYPMSSEIK 1051 IYYNELQNKP DAKKARFWSF MKTQRFVSNM GFDIQKSCEP VSISTSVKPH 1101 VVEPEHMADA KIMPKDILQI TKKPLMVKNV KPSSPPDVKS LVQLSTMETK 1151 TLPEKKQFDS IFNSNKAKII PGNGKHASEN ISLSFSRPAS YGYFSVGKRV 1201 PIVEDRRVKQ LDDITDSNTT EILTSVDVLG THSQTGTQQS NMYTSTQKTE 1251 LEIDNKDSVT ECSKDMKEDG LSFVDIVLSK AASALDEKEK QLAVANEIIR 1301 SLSDEVMRNE IRITSLQGDL TFTKKCLENA RSQISEKDAK INKLMEKDFQ 1351 VNKEIKPY*

Amino Acid Sequence (or in FASTA format)

       1  MPNDNKTPNR SSTPKFTKKP VTPNDKIPER EEKSNEVKTP KIPLFTFAKS
      51  KNYSRPSTAI HTSPHQPSDV KPTSHKQLQQ PKSSPLKKNN YNSFPHSNLE
     101  KISNSKLLSL LRSKTSAGRI ESNNPSHDAS RSLASFEQTA FSRHAQQQTS
     151  TFNSKPVRTI VPISTSQTNN SFLSGVKSLL SEEKIRDYSK EILGINLANE
     201  QPVLEKPLKK GSADIGASVI SLTKDKSIRK DTVEEKKEEK LNIGKNFAHS
     251  DSLSVPKVSA GDSGISPEES KARSPGIAKP NAIQTEVYGI NEESTNERLE
     301  INQEKPVKLD ENSANSTVAS ALDTNGTSAT TETLTSKKIV PSPKKVAIDQ
     351  DKITLHDEKT LAPSKHQPIT SEQKMKEDAD LKRMEILKSP HLSKSPADRP
     401  QGRRNSRNFS TRDEETTKLA FLVEYEGQEN NYNSTSRSTE KKNDMNTSAK
     451  NKNGENKKIG KRPPEIMSTE AHVNKVTEET TKQIQSVRID GRKVLQKVQG
     501  ESHIDSRNNT LNVTPSKRPQ LGEIPNPMKK HKPNEGRTPN ISNGTINIQK
     551  KLEPKEIVRD ILHTKESSNE AKKTIQNPLN KSQNTALPST HKVTQKKDIK
     601  IGTNDLFQVE SAPKISSEID RENVKSKDEP VSKAVESKSL LNLFSNVLKA
     651  PFIKSESKPF SSDALSKEKA NFLETIASTE KPENKTDKVS LSQPVSASKH
     701  EYSDNFPVSL SQPSKKSFAN HTEDEQIEKK KICRGRMNTI ITHPGKMELV
     751  YVSDSDDSSS DNDSLTDLES LSSGESNEIK VTNDLDTSAE KDQIQAGKWF
     801  DPVLDWRKSD RELTKNILWR IADKTTYDKE TITDLIEQGI PKHSYLSGNP
     851  LTSVTNDICS VENYETSSAF FYQQVHKKDR LQYLPLYAVS TFENTNNTEK
     901  NDVTNKNINI GKHSQEQNSS SAKPSQIPTV SSPLGFEETK LSTTPTKSNR
     951  RVSHSDTNSS KPKNTKENLS KSSWRQEWLA NLKLISVSLV DEFPSELSDS
    1001  DRQIINEKMQ LLKDIFANNL KSAISNNFRE SDIIILKGEI EDYPMSSEIK
    1051  IYYNELQNKP DAKKARFWSF MKTQRFVSNM GFDIQKSCEP VSISTSVKPH
    1101  VVEPEHMADA KIMPKDILQI TKKPLMVKNV KPSSPPDVKS LVQLSTMETK
    1151  TLPEKKQFDS IFNSNKAKII PGNGKHASEN ISLSFSRPAS YGYFSVGKRV
    1201  PIVEDRRVKQ LDDITDSNTT EILTSVDVLG THSQTGTQQS NMYTSTQKTE
    1251  LEIDNKDSVT ECSKDMKEDG LSFVDIVLSK AASALDEKEK QLAVANEIIR
    1301  SLSDEVMRNE IRITSLQGDL TFTKKCLENA RSQISEKDAK INKLMEKDFQ
    1351  VNKEIKPY*

external links for Sir4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Sir4p

1)	Rine J and Herskowitz I (1987) Four genes responsible for a position effect on expression from HML and HMR in Saccharomyces cerevisiae. Genetics 116(1):9-22
2)	Kennedy BK, et al. (1995) Mutation in the silencing gene SIR4 can delay aging in S. cerevisiae. Cell 80(3):485-96
3)	Moazed D, et al. (1997) Silent information regulator protein complexes in Saccharomyces cerevisiae: a SIR2/SIR4 complex and evidence for a regulatory domain in SIR4 that inhibits its interaction with SIR3. Proc Natl Acad Sci U S A 94(6):2186-91
4)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64