GCD6/YDR211W Protein Information Help

Standard Name Gcd6p 1
Systematic Name Ydr211wp
ORF Classification Verified
Description Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress (2, 3, 4)
Name Description General Control Derepressed 5
Experimental Data
Molecules/cell 33800 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 712
Molecular Weight (Da) 81,160
Isoelectric Point (pI) 4.48

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Gcd6p (InterPro)
Physical Interactions There are 125 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052E48 | P32501
MIPS: YDR211W
NCBI: 1122344 | 1204152 | 171574 | 398366139 | 417035 | 46015194 | NP_010497.3 | NM_001180519.3
GenBank/EMBL/DDBJ: DAA12055.1 | L07115 | Z68194 | Z68195
Amino Acid Sequence (or in FASTA format)
 
       1  MAGKKGQKKS GLGNHGKNSD MDVEDRLQAV VLTDSYETRF MPLTAVKPRC
      51  LLPLANVPLI EYTLEFLAKA GVHEVFLICS SHANQINDYI ENSKWNLPWS
     101  PFKITTIMSP EARCTGDVMR DLDNRGIITG DFILVSGDVL TNIDFSKMLE
     151  FHKKMHLQDK DHISTMCLSK ASTYPKTRTI EPAAFVLDKS TSRCIYYQDL
     201  PLPSSREKTS IQIDPELLDN VDEFVIRNDL IDCRIDICTS HVPLIFQENF
     251  DYQSLRTDFV KGVISSDILG KHIYAYLTDE YAVRVESWQT YDTISQDFLG
     301  RWCYPLVLDS NIQDDQTYSY ESRHIYKEKD VVLAQSCKIG KCTAIGSGTK
     351  IGEGTKIENS VIGRNCQIGE NIRIKNSFIW DDCIIGNNSI IDHSLIASNA
     401  TLGSNVRLND GCIIGFNVKI DDNMDLDRNT KISASPLKNA GSRMYDNESN
     451  EQFDQDLDDQ TLAVSIVGDK GVGYIYESEV SDDEDSSTEA CKEINTLSNQ
     501  LDELYLSDDS ISSATKKTKK RRTMSVNSIY TDREEIDSEF EDEDFEKEGI
     551  ATVERAMENN HDLDTALLEL NTLRMSMNVT YHEVRIATIT ALLRRVYHFI
     601  ATQTLGPKDA VVKVFNQWGL LFKRQAFDEE EYIDLMNIIM EKIVEQSFDK
     651  PDLILFSALV SLYDNDIIEE DVIYKWWDNV STDPRYDEVK KLTVKWVEWL
     701  QNADEESSSE EE*

external links for Gcd6p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Gcd6p
1) Niederberger P, et al.  (1986) Identification and characterization of four new GCD genes in Saccharomyces cerevisiae. Curr Genet 10(9):657-64
2) Pavitt GD, et al.  (1998) eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange. Genes Dev 12(4):514-26
3) Hinnebusch A  (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Lucchini G, et al.  (1984) Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae. Mol Cell Biol 4(7):1326-33
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41