UME6/YDR207C Protein Information

Standard Name	Ume6p 1
Systematic Name	Ydr207cp
Alias	Car80p 1, 2 , Nim2p , Rim16p 3
ORF Classification	Verified
Description	Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p (10, 2, 4, 5, 6, 7, 8, 9)
Name Description	Unscheduled Meiotic gene Expression 11

Experimental Data
Molecules/cell	217 12

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	836
Molecular Weight (Da)	91,123
Isoelectric Point (pI)	10.32

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ume6p (InterPro)
Physical Interactions	There are 64 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005313A \| P39001 MIPS: YDR207C NCBI: 1122335 \| 1718025 \| 435118 \| 457408 \| 479019 \| 6320413 \| NP_010493.1 GenBank/EMBL/DDBJ: DAA12048.1 \| D23663 \| L24539 \| L32186 \| Z68194

Amino Acid Sequence (or in FASTA format)
1 MLDKARSQSK HMDESNAAAS LLSMETTANN HHYLHNKTSR ATLMNSSQDG 51 KKHAEDEVSD GANSRHPTIS SASIESLKTT YDENPLLSIM KSTCAPNNTP 101 VHTPSGSPSL KVQSGGDIKD DPKENDTTTT TNTTLQDRRD SDNAVHAAAS 151 PLAPSNTPSD PKSLCNGHVA QATDPQISGA IQPQYTATNE DVFPYSSTST 201 NSNTATTTIV AGAKKKIHLP PPQAPAVSSP GTTAAGSGAG TGSGIRSRTG 251 SDLPLIITSA NKNNGKTTNS PMSILSRNNS TNNNDNNSIQ SSDSRESSNN 301 NEIGGYLRGG TKRGGSPSND SQVQHNVHDD QCAVGVAPRN FYFNKDREIT 351 DPNVKLDENE SKINISFWLN SKYRDEAYSL NESSSNNASS NTDTPTNSRH 401 ANTSSSITSR NNFQHFRFNQ IPSQPPTSAS SFTSTNNNNP QRNNINRGED 451 PFATSSRPST GFFYGDLPNR NNRNSPFHTN EQYIPPPPPK YINSKLDGLR 501 SRLLLGPNSA SSSTKLDDDL GTAAAVLSNM RSSPYRTHDK PISNVNDMNN 551 TNALGVPASR PHSSSFPSKG VLRPILLRIH NSEQQPIFES NNSTAVFDED 601 QDQNQDLSPY HLNLNSKKVL DPTFESRTRQ VTWNKNGKRI DRRLSAPEQQ 651 QQLEVPPLKK SRRSVGNARV ASQTNSDYNS LGESSTSSAP SSPSLKASSG 701 LAYTADYPNA TSPDFAKSKG KNVKPKAKSK AKQSSKKRPN NTTSKSKANN 751 SQESNNATSS TSQGTRSRTG CWICRLRKKK CTEERPHCFN CERLKLDCHY 801 DAFKPDFVSD PKKKQMKLEE IKKKTKEAKR RAMKKK*

Amino Acid Sequence (or in FASTA format)

       1  MLDKARSQSK HMDESNAAAS LLSMETTANN HHYLHNKTSR ATLMNSSQDG
      51  KKHAEDEVSD GANSRHPTIS SASIESLKTT YDENPLLSIM KSTCAPNNTP
     101  VHTPSGSPSL KVQSGGDIKD DPKENDTTTT TNTTLQDRRD SDNAVHAAAS
     151  PLAPSNTPSD PKSLCNGHVA QATDPQISGA IQPQYTATNE DVFPYSSTST
     201  NSNTATTTIV AGAKKKIHLP PPQAPAVSSP GTTAAGSGAG TGSGIRSRTG
     251  SDLPLIITSA NKNNGKTTNS PMSILSRNNS TNNNDNNSIQ SSDSRESSNN
     301  NEIGGYLRGG TKRGGSPSND SQVQHNVHDD QCAVGVAPRN FYFNKDREIT
     351  DPNVKLDENE SKINISFWLN SKYRDEAYSL NESSSNNASS NTDTPTNSRH
     401  ANTSSSITSR NNFQHFRFNQ IPSQPPTSAS SFTSTNNNNP QRNNINRGED
     451  PFATSSRPST GFFYGDLPNR NNRNSPFHTN EQYIPPPPPK YINSKLDGLR
     501  SRLLLGPNSA SSSTKLDDDL GTAAAVLSNM RSSPYRTHDK PISNVNDMNN
     551  TNALGVPASR PHSSSFPSKG VLRPILLRIH NSEQQPIFES NNSTAVFDED
     601  QDQNQDLSPY HLNLNSKKVL DPTFESRTRQ VTWNKNGKRI DRRLSAPEQQ
     651  QQLEVPPLKK SRRSVGNARV ASQTNSDYNS LGESSTSSAP SSPSLKASSG
     701  LAYTADYPNA TSPDFAKSKG KNVKPKAKSK AKQSSKKRPN NTTSKSKANN
     751  SQESNNATSS TSQGTRSRTG CWICRLRKKK CTEERPHCFN CERLKLDCHY
     801  DAFKPDFVSD PKKKQMKLEE IKKKTKEAKR RAMKKK*

external links for Ume6p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ume6p

1)	Park HD, et al. (1992) The yeast UME6 gene product is required for transcriptional repression mediated by the CAR1 URS1 repressor binding site. Nucleic Acids Res 20(8):1909-15
2)	Strich R, et al. (1994) UME6 is a key regulator of nitrogen repression and meiotic development. Genes Dev 8(7):796-810
3)	Mitchell AP and Bowdish KS (1992) Selection for early meiotic mutants in yeast. Genetics 131(1):65-72
4)	Anderson SF, et al. (1995) UME6, a negative regulator of meiosis in Saccharomyces cerevisiae, contains a C-terminal Zn2Cys6 binuclear cluster that binds the URS1 DNA sequence in a zinc-dependent manner. Protein Sci 4(9):1832-43
5)	Bowdish KS and Mitchell AP (1993) Bipartite structure of an early meiotic upstream activation sequence from Saccharomyces cerevisiae. Mol Cell Biol 13(4):2172-81
6)	Steber CM and Esposito RE (1995) UME6 is a central component of a developmental regulatory switch controlling meiosis-specific gene expression. Proc Natl Acad Sci U S A 92(26):12490-4
7)	Malathi K, et al. (1997) Interaction of yeast repressor-activator protein Ume6p with glycogen synthase kinase 3 homolog Rim11p. Mol Cell Biol 17(12):7230-6
8)	Messenguy F, et al. (2000) In Saccharomyces cerevisiae, expression of arginine catabolic genes CAR1 and CAR2 in response to exogenous nitrogen availability is mediated by the Ume6 (CargRI)-Sin3 (CargRII)-Rpd3 (CargRIII) complex. J Bacteriol 182(11):3158-64
9)	Elkhaimi M, et al. (2000) Combinatorial regulation of phospholipid biosynthetic gene expression by the UME6, SIN3 and RPD3 genes. Nucleic Acids Res 28(16):3160-7
10)	Williams RM, et al. (2002) The Ume6 regulon coordinates metabolic and meiotic gene expression in yeast. Proc Natl Acad Sci U S A 99(21):13431-6
11)	Strich R, et al. (1989) Identification of negative regulatory genes that govern the expression of early meiotic genes in yeast. Proc Natl Acad Sci U S A 86(24):10018-22
12)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41