RVB1/YDR190C Protein Information

Standard Name	Rvb1p
Systematic Name	Ydr190cp
Alias	Tip49ap 1 , Tih1p 2 , Tip49p 3
ORF Classification	Verified
Description	ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly (10, 11, 12, 13, 14, 15, 4, 5, 6, 7, 8, 9)
Name Description	RuVB-like 6

Experimental Data
Molecules/cell	11600 16

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	463
Molecular Weight (Da)	50,453
Isoelectric Point (pI)	5.7

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rvb1p (InterPro)
Physical Interactions	There are 186 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000069A8B \| Q03940 MIPS: YDR190C NCBI: 6320396 \| 73919282 \| 755784 \| NP_010476.1 \| NM_001180498.1 GenBank/EMBL/DDBJ: DAA12033.1 \| Z48784
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.12 [DNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MVAISEVKEN PGVNSSNSGA VTRTAAHTHI KGLGLDESGV AKRVEGGFVG 51 QIEAREACGV IVDLIKAKKM SGRAILLAGG PSTGKTALAL AISQELGPKV 101 PFCPLVGSEL YSVEVKKTET LMENFRRAIG LRIKETKEVY EGEVTELTPE 151 DAENPLGGYG KTISHVIVGL KSAKGTKTLR LDPTIYESIQ REKVSIGDVI 201 YIEANTGAVK RVGRSDAYAT EFDLETEEYV PLPKGEVHKK KEIVQDVTLH 251 DLDVANARPQ GGQDVISMMG QLLKPKKTEI TEKLRQEVNK VVAKYIDQGV 301 AELIPGVLFI DEVNMLDIEI FTYLNKALES NIAPVVVLAS NRGMTTVRGT 351 EDVISPHGVP PDLIDRLLIV RTLPYDKDEI RTIIERRATV ERLQVESSAL 401 DLLATMGTET SLRYALQLLA PCGILAQTSN RKEIVVNDVN EAKLLFLDAK 451 RSTKILETSA NYL*

Amino Acid Sequence (or in FASTA format)

       1  MVAISEVKEN PGVNSSNSGA VTRTAAHTHI KGLGLDESGV AKRVEGGFVG
      51  QIEAREACGV IVDLIKAKKM SGRAILLAGG PSTGKTALAL AISQELGPKV
     101  PFCPLVGSEL YSVEVKKTET LMENFRRAIG LRIKETKEVY EGEVTELTPE
     151  DAENPLGGYG KTISHVIVGL KSAKGTKTLR LDPTIYESIQ REKVSIGDVI
     201  YIEANTGAVK RVGRSDAYAT EFDLETEEYV PLPKGEVHKK KEIVQDVTLH
     251  DLDVANARPQ GGQDVISMMG QLLKPKKTEI TEKLRQEVNK VVAKYIDQGV
     301  AELIPGVLFI DEVNMLDIEI FTYLNKALES NIAPVVVLAS NRGMTTVRGT
     351  EDVISPHGVP PDLIDRLLIV RTLPYDKDEI RTIIERRATV ERLQVESSAL
     401  DLLATMGTET SLRYALQLLA PCGILAQTSN RKEIVVNDVN EAKLLFLDAK
     451  RSTKILETSA NYL*

external links for Rvb1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Rvb1p

1)	Kanemaki M, et al. (1999) TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a. J Biol Chem 274(32):22437-44
2)	Lim CR, et al. (2000) The Saccharomyces cerevisiae RuvB-like protein, Tih2p, is required for cell cycle progression and RNA polymerase II-directed transcription. J Biol Chem 275(29):22409-17
3)	Wood MA, et al. (2000) An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc. Mol Cell 5(2):321-30
4)	Shen X, et al. (2000) A chromatin remodelling complex involved in transcription and DNA processing. Nature 406(6795):541-4
5)	Qiu XB, et al. (1998) An eukaryotic RuvB-like protein (RUVBL1) essential for growth. J Biol Chem 273(43):27786-93
6)	Jonsson ZO, et al. (2001) Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes. J Biol Chem 276(19):16279-88
7)	King TH, et al. (2001) A well-connected and conserved nucleoplasmic helicase is required for production of box C/D and H/ACA snoRNAs and localization of snoRNP proteins. Mol Cell Biol 21(22):7731-46
8)	Krogan NJ, et al. (2003) A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1. Mol Cell 12(6):1565-76
9)	Mizuguchi G, et al. (2004) ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex. Science 303(5656):343-8
10)	Jonsson ZO, et al. (2004) Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex. Mol Cell 16(3):465-77
11)	Zhao R, et al. (2005) Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone. Cell 120(5):715-27
12)	Gribun A, et al. (2008) Yeast Rvb1 and Rvb2 are ATP-dependent DNA helicases that form a heterohexameric complex. J Mol Biol 376(5):1320-33
13)	Torreira E, et al. (2008) Architecture of the pontin/reptin complex, essential in the assembly of several macromolecular complexes. Structure 16(10):1511-20
14)	Zhao R, et al. (2008) Molecular chaperone Hsp90 stabilizes Pih1/Nop17 to maintain R2TP complex activity that regulates snoRNA accumulation. J Cell Biol 180(3):563-78
15)	Kakihara Y and Houry WA (2012) The R2TP complex: discovery and functions. Biochim Biophys Acta 1823(1):101-7
16)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41