UBC1/YDR177W Protein Information

Standard Name	Ubc1p
Systematic Name	Ydr177wp
ORF Classification	Verified
Description	Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5)
Name Description	UBiquitin-Conjugating

Experimental Data
Molecules/cell	8940 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	215
Molecular Weight (Da)	24,178
Isoelectric Point (pI)	4.97

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ubc1p (InterPro)
Physical Interactions	There are 19 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000137952 \| P21734 MIPS: YDR177W NCBI: 1289292 \| 136635 \| 16975327 \| 16975401 \| 16975402 \| 398365965 \| 45269243 \| 4717 \| 52695852 \| NP_010462.3 GenBank/EMBL/DDBJ: DAA12019.1 \| AY557675 \| X56402 \| Z46727
External Classifications	EC: 6.3.2.19 [Ubiquitin--protein ligase]

Amino Acid Sequence (or in FASTA format)
1 MSRAKRIMKE IQAVKDDPAA HITLEFVSES DIHHLKGTFL GPPGTPYEGG 51 KFVVDIEVPM EYPFKPPKMQ FDTKVYHPNI SSVTGAICLD ILKNAWSPVI 101 TLKSALISLQ ALLQSPEPND PQDAEVAQHY LRDRESFNKT AALWTRLYAS 151 ETSNGQKGNV EESDLYGIDH DLIDEFESQG FEKDKIVEVL RRLGVKSLDP 201 NDNNTANRII EELLK*

Amino Acid Sequence (or in FASTA format)

       1  MSRAKRIMKE IQAVKDDPAA HITLEFVSES DIHHLKGTFL GPPGTPYEGG
      51  KFVVDIEVPM EYPFKPPKMQ FDTKVYHPNI SSVTGAICLD ILKNAWSPVI
     101  TLKSALISLQ ALLQSPEPND PQDAEVAQHY LRDRESFNKT AALWTRLYAS
     151  ETSNGQKGNV EESDLYGIDH DLIDEFESQG FEKDKIVEVL RRLGVKSLDP
     201  NDNNTANRII EELLK*

external links for Ubc1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Ubc1p

1)	Seufert W and Jentsch S (1991) Yeast ubiquitin-conjugating enzymes involved in selective protein degradation are essential for cell viability. Acta Biol Hung 42(1-3):27-37
2)	Friedlander R, et al. (2000) A regulatory link between ER-associated protein degradation and the unfolded-protein response. Nat Cell Biol 2(7):379-84
3)	Seufert W, et al. (1990) UBC1 encodes a novel member of an essential subfamily of yeast ubiquitin-conjugating enzymes involved in protein degradation. EMBO J 9(13):4535-41
4)	Shieh HL, et al. (2001) Biochemical analysis of fructose-1,6-bisphosphatase import into vacuole import and degradation vesicles reveals a role for UBC1 in vesicle biogenesis. J Biol Chem 276(13):10398-406
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41