ARG82/YDR173C Protein Information Help

Standard Name Arg82p 1
Systematic Name Ydr173cp
Alias Argr3p , Ipk2p 2
ORF Classification Verified
Description Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes (2, 3, 4, 5, 6, 7) Also known as: ARGRIII 8
Name Description ARGinine requiring
Experimental Data
Molecules/cell 2720 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 355
Molecular Weight (Da) 40,353
Isoelectric Point (pI) 4.41

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Arg82p (InterPro)
Physical Interactions There are 16 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000125DFA | P07250
MIPS: YDR173C
NCBI: 114134 | 118138345 | 118138346 | 118138352 | 118138353 | 1289288 | 3376 | 398365957 | 45269285 | NP_010458.3
GenBank/EMBL/DDBJ: DAA12015.1 | AY557696 | X05328 | Z46727
External Classifications EC: 2.7.1.151 [Inositol-polyphosphate multikinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MDTVNNYRVL EHKAAGHDGT LTDGDGLLIF KPAFPQELEF YKAIQVRDVS
      51  RRKSSADGDA PLCSWMPTYL GVLNEGAKIE QSGDAALLKI DERLSDSTDN
     101  LDSIPVKSEK SKQYLVLENL LYGFSKPNIL DIKLGKTLYD SKASLEKRER
     151  MKRVSETTTS GSLGFRICGM KIQKNPSVLN QLSLEYYEEE ADSDYIFINK
     201  LYGRSRTDQN VSDAIELYFN NPHLSDARKH QLKKTFLKRL QLFYNTMLEE
     251  EVRMISSSLL FIYEGDPERW ELLNDVDKLM RDDFIDDDDD DDDNDDDDDD
     301  DAEGSSEGPK DKKTTGSLSS MSLIDFAHSE ITPGKGYDEN VIEGVETLLD
     351  IFMKF*

external links for Arg82p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Arg82p
1) Deschamps J and Wiame JM  (1979) Mating-type effect on cis mutations leading to constitutivity of ornithine transaminase in diploid cells of Saccharomyces cerevisiae. Genetics 92(3):749-58
2) Odom AR, et al.  (2000) A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional control. Science 287(5460):2026-9
3) Dubois E, et al.  (2000) Inositol polyphosphate kinase activity of Arg82/ArgRIII is not required for the regulation of the arginine metabolism in yeast. FEBS Lett 486(3):300-4
4) Zhang T, et al.  (2001) The transcriptional regulator, Arg82, is a hybrid kinase with both monophosphoinositol and diphosphoinositol polyphosphate synthase activity. FEBS Lett 494(3):208-12
5) Saiardi A, et al.  (1999) Synthesis of diphosphoinositol pentakisphosphate by a newly identified family of higher inositol polyphosphate kinases. Curr Biol 9(22):1323-6
6) El Alami M, et al.  (2003) Arg82p is a bifunctional protein whose inositol polyphosphate kinase activity is essential for nitrogen and PHO gene expression but not for Mcm1p chaperoning in yeast. Mol Microbiol 49(2):457-68
7) Qui HF, et al.  (1991) Dissection of the bifunctional ARGRII protein involved in the regulation of arginine anabolic and catabolic pathways. Mol Cell Biol 11(4):2169-79
8) Bechet J, et al.  (1970) Mutations affecting the repressibility of arginine biosynthetic enzymes in Saccharomyces cerevisiae. Eur J Biochem 12(1):31-9
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41