HSP42/YDR171W Protein Information

Standard Name	Hsp42p
Systematic Name	Ydr171wp
ORF Classification	Verified
Description	Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress (1, 2, 3, 4, 5)
Name Description	Heat Shock Protein 2

Experimental Data
Molecules/cell	1470 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	375
Molecular Weight (Da)	42,817
Isoelectric Point (pI)	4.84

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hsp42p (InterPro)
Physical Interactions	There are 74 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004F8F3 \| Q12329 MIPS: YDR171W NCBI: 1163915 \| 1289286 \| 2495335 \| 45271038 \| 6320376 \| NP_010456.1 GenBank/EMBL/DDBJ: DAA12013.1 \| AY558574 \| U41401 \| Z46727

Amino Acid Sequence (or in FASTA format)
1 MSFYQPSLSL YDVLNALSNQ TGQRGQQGYP RQPQRPQRYH PHYGQVHVGG 51 HHPRHHPLYS RYNGVPNTYY YQFPGQAYYY SPEYGYDDED GEEEDQDEDM 101 VGDSGTTRQE DGGEDSNSRR YPSYYHCNTA RNNRTNQQAN SLNDLLTALI 151 GVPPYEGTEP EIEANTEQEG EKGEEKDKKD KSEAPKEEAG ETNKEKPLNQ 201 LEESSRPPLA KKSSSFAHLQ APSPIPDPLQ VSKPETRMDL PFSPEVNVYD 251 TEDTYVVVLA LPGANSRAFH IDYHPSSHEM LIKGKIEDRV GIDEKFLKIT 301 ELKYGAFERT VKFPVLPRIK DEEIKATYNN GLLQIKVPKI VNDTEKPKPK 351 KRIAIEEIPD EELEFEENPN PTVEN*

Amino Acid Sequence (or in FASTA format)

       1  MSFYQPSLSL YDVLNALSNQ TGQRGQQGYP RQPQRPQRYH PHYGQVHVGG
      51  HHPRHHPLYS RYNGVPNTYY YQFPGQAYYY SPEYGYDDED GEEEDQDEDM
     101  VGDSGTTRQE DGGEDSNSRR YPSYYHCNTA RNNRTNQQAN SLNDLLTALI
     151  GVPPYEGTEP EIEANTEQEG EKGEEKDKKD KSEAPKEEAG ETNKEKPLNQ
     201  LEESSRPPLA KKSSSFAHLQ APSPIPDPLQ VSKPETRMDL PFSPEVNVYD
     251  TEDTYVVVLA LPGANSRAFH IDYHPSSHEM LIKGKIEDRV GIDEKFLKIT
     301  ELKYGAFERT VKFPVLPRIK DEEIKATYNN GLLQIKVPKI VNDTEKPKPK
     351  KRIAIEEIPD EELEFEENPN PTVEN*

external links for Hsp42p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Hsp42p

1)	Gu J, et al. (1997) Small heat shock protein suppression of Vpr-induced cytoskeletal defects in budding yeast. Mol Cell Biol 17(7):4033-42
2)	Wotton D, et al. (1996) Multimerization of Hsp42p, a novel heat shock protein of Saccharomyces cerevisiae, is dependent on a conserved carboxyl-terminal sequence. J Biol Chem 271(5):2717-23
3)	Trotter EW, et al. (2002) Misfolded proteins are competent to mediate a subset of the responses to heat shock in Saccharomyces cerevisiae. J Biol Chem 277(47):44817-25
4)	Haslbeck M, et al. (2004) Hsp42 is the general small heat shock protein in the cytosol of Saccharomyces cerevisiae. EMBO J 23(3):638-49
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41