SEC7/YDR170C Protein Information

Standard Name	Sec7p 1
Systematic Name	Ydr170cp
ORF Classification	Verified
Description	Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles (2, 3, 4)
Name Description	SECretory 5

Experimental Data
Molecules/cell	3670 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	2,009
Molecular Weight (Da)	226,884
Isoelectric Point (pI)	4.51

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sec7p (InterPro)
Physical Interactions	There are 76 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000135756 \| P11075 MIPS: YDR170C NCBI: 1204159 \| 1326010 \| 172570 \| 2507125 \| 398365941 \| NP_010454.3 \| NM_001180477.3 GenBank/EMBL/DDBJ: DAA12011.1 \| J03918 \| Z46727 \| Z47813

Amino Acid Sequence (or in FASTA format)
1 MSEQNSVVNA EKGDGEISSN VETASSVNPS VKPQNAIKEE AKETNGEDQK 51 CKGPENAGST AETKETSNDA TNGMKTPEET EDTNDKRHDD EGEDGDEDED 101 EDEDEDEDNG DEDDEDVDSS SSETSSEDGE DSESVSGEST ESSSGEDEES 151 DESDGNTSNS SSGDESGSEE EEEEEEEEEE EENAGEPAIA HQDSVPTNDS 201 TAPRSTHTRN ISLSSNGSNT NSTIILVKTT LETILNDKDI KKNSNAQKAI 251 ERTLQKFKEF DPQTTNNPHY VDSILVFEAL RASCRTKSSK VQSLALDCLS 301 KLFSFRSLDE TLLVNPPDSL ASNDQRQDAA DGITPPPKQK IIDAAIDTIS 351 DCFQGEGTDD RVELQIVRAL SSCILEEDSS SLCHGASLLK AIRTIYNVFV 401 FSLNPSNQGI AQATLTQIIS SVYDKIDLKQ STSSAVSLST KNHQQQSAIE 451 LSEASENAET PAPLTLENMD KLNDDEERLM DAQQPDSIAI TNQDLAVKDA 501 FLVFRVMAKI CAKPLETELD MRSHAVRSKL LSLHIIYSII KDHIDVFLSH 551 NIFLPGKERV CFIDSIRQYL RLVLSRNAAS PLAPVFEVTL EIMWLLIANL 601 RADFVKEIPV FLTEIYFPIS ELTTSTSQQK RYFLSVIQRI CNDPRTLVEF 651 YLNYDCNPGM PNVMEITVDY LTRLALTRVE ITQTQRSYYD EQISKSLSTY 701 NFSQLPLLTS SNLSSSPDVG QVNLLFPLDF ALKMVSLNCI VSVLRSLSSW 751 AHKALNPNTH TANKVLLNTT SSARQESRSS LSNDVRSSIM TSNDDFKPTY 801 EDEESRSLSS QNIDADDPTQ FENLKLRKTA LSECIAIFNN KPKKAIPVLI 851 KKGFLKDDSP ISIAKWLLET EGLDMAAVGD YLGEGDDKNI AIMHAFVDEF 901 DFTGMSIVDA LRSFLQSFRL PGEGQKIDRF MLKFAERFVD QNPGVFSKAD 951 TAYVLSYSLI MLNTDLHSSQ IKNKMSLQEF LENNEGIDNG RDLPRDFLEG 1001 LFNEIANNEI KLISEQHQAM LSGDTNLVQQ QQSAFNFFNS RDLTREAYNQ 1051 VSKEISSKTE LVFKNLNKNK GGPDVYYAAS HVEHVKSIFE TLWMSFLAAL 1101 TPPFKDYDDI DTTNKCLEGL KISIKIASTF RINDARTSFV GALVQFCNLQ 1151 NLEEIKVKNV NAMVILLEVA LSEGNYLEGS WKDILLVVSQ MERLQLISKG 1201 IDRDTVPDVA QARVANPRVS YESSRSNNTS FFDVWGKKAT PTELAQEKHH 1251 NQTLSPEISK FISSSELVVL MDNIFTKSSE LSGNAIVDFI KALTAVSLEE 1301 IESSENASTP RMFSLQKMVD VCYYNMDRIK LEWTPLWAVM GKAFNKIATN 1351 SNLAVVFFAI DSLRQLSMRF LDIEELSGFE FQHDFLKPFE YTVQNSGNTE 1401 VQEMIIECFR NFILTKSESI KSGWKPILES LQYTARSSTE SIVLKTQLLV 1451 SNDIVTNHFE NVFSQEDAFS ELVGVFREIT KNKRFQKLSL HALESLRKMT 1501 QNVADICFYN ENKTEEERKH NDALLRGKDI FQDVWFPMLF CFNDTIMTAE 1551 DLEVRSRALN YMFDALVAYG GKFNDDFWEK ICKKLLFPIF GVLSKHWEVN 1601 QFNSHDDLSV WLSTTLIQAL RNLIALFTHY FESLNRMLDG FLGLLVSCIC 1651 QENDTIARIG RSCLQQLILQ NVSKFNEYHW NQIGDVFDKL FDLTTANELF 1701 DYDPLQQGRK SSVSHHQTTN DTSQHSDDDS NDRRENDSNI SETVERAHQE 1751 ESSEDVGGDM VETLNGQTKL NNGNSVPTVK DELNPKPASL SIPKKTKHMK 1801 RNESNEDIRR RINIKNSIVV KCVLQLLMIE LLNELFENED FAHCIPYKEA 1851 IRITRLLEKS YEFSRDFNED YGLRTRLVEA RVVDKIPNLL KQETSAAAVL 1901 LDIMFQLYLN DDEKKADLIT RLITICIQVV EGYVSLDDRT MERSINAWRS 1951 VIVEILQGYY EFDDEDFRLY CPAMYALVIQ ILDKSVPTEL RHAIKQFLSR 2001 VGELYLSTD*

Amino Acid Sequence (or in FASTA format)

       1  MSEQNSVVNA EKGDGEISSN VETASSVNPS VKPQNAIKEE AKETNGEDQK
      51  CKGPENAGST AETKETSNDA TNGMKTPEET EDTNDKRHDD EGEDGDEDED
     101  EDEDEDEDNG DEDDEDVDSS SSETSSEDGE DSESVSGEST ESSSGEDEES
     151  DESDGNTSNS SSGDESGSEE EEEEEEEEEE EENAGEPAIA HQDSVPTNDS
     201  TAPRSTHTRN ISLSSNGSNT NSTIILVKTT LETILNDKDI KKNSNAQKAI
     251  ERTLQKFKEF DPQTTNNPHY VDSILVFEAL RASCRTKSSK VQSLALDCLS
     301  KLFSFRSLDE TLLVNPPDSL ASNDQRQDAA DGITPPPKQK IIDAAIDTIS
     351  DCFQGEGTDD RVELQIVRAL SSCILEEDSS SLCHGASLLK AIRTIYNVFV
     401  FSLNPSNQGI AQATLTQIIS SVYDKIDLKQ STSSAVSLST KNHQQQSAIE
     451  LSEASENAET PAPLTLENMD KLNDDEERLM DAQQPDSIAI TNQDLAVKDA
     501  FLVFRVMAKI CAKPLETELD MRSHAVRSKL LSLHIIYSII KDHIDVFLSH
     551  NIFLPGKERV CFIDSIRQYL RLVLSRNAAS PLAPVFEVTL EIMWLLIANL
     601  RADFVKEIPV FLTEIYFPIS ELTTSTSQQK RYFLSVIQRI CNDPRTLVEF
     651  YLNYDCNPGM PNVMEITVDY LTRLALTRVE ITQTQRSYYD EQISKSLSTY
     701  NFSQLPLLTS SNLSSSPDVG QVNLLFPLDF ALKMVSLNCI VSVLRSLSSW
     751  AHKALNPNTH TANKVLLNTT SSARQESRSS LSNDVRSSIM TSNDDFKPTY
     801  EDEESRSLSS QNIDADDPTQ FENLKLRKTA LSECIAIFNN KPKKAIPVLI
     851  KKGFLKDDSP ISIAKWLLET EGLDMAAVGD YLGEGDDKNI AIMHAFVDEF
     901  DFTGMSIVDA LRSFLQSFRL PGEGQKIDRF MLKFAERFVD QNPGVFSKAD
     951  TAYVLSYSLI MLNTDLHSSQ IKNKMSLQEF LENNEGIDNG RDLPRDFLEG
    1001  LFNEIANNEI KLISEQHQAM LSGDTNLVQQ QQSAFNFFNS RDLTREAYNQ
    1051  VSKEISSKTE LVFKNLNKNK GGPDVYYAAS HVEHVKSIFE TLWMSFLAAL
    1101  TPPFKDYDDI DTTNKCLEGL KISIKIASTF RINDARTSFV GALVQFCNLQ
    1151  NLEEIKVKNV NAMVILLEVA LSEGNYLEGS WKDILLVVSQ MERLQLISKG
    1201  IDRDTVPDVA QARVANPRVS YESSRSNNTS FFDVWGKKAT PTELAQEKHH
    1251  NQTLSPEISK FISSSELVVL MDNIFTKSSE LSGNAIVDFI KALTAVSLEE
    1301  IESSENASTP RMFSLQKMVD VCYYNMDRIK LEWTPLWAVM GKAFNKIATN
    1351  SNLAVVFFAI DSLRQLSMRF LDIEELSGFE FQHDFLKPFE YTVQNSGNTE
    1401  VQEMIIECFR NFILTKSESI KSGWKPILES LQYTARSSTE SIVLKTQLLV
    1451  SNDIVTNHFE NVFSQEDAFS ELVGVFREIT KNKRFQKLSL HALESLRKMT
    1501  QNVADICFYN ENKTEEERKH NDALLRGKDI FQDVWFPMLF CFNDTIMTAE
    1551  DLEVRSRALN YMFDALVAYG GKFNDDFWEK ICKKLLFPIF GVLSKHWEVN
    1601  QFNSHDDLSV WLSTTLIQAL RNLIALFTHY FESLNRMLDG FLGLLVSCIC
    1651  QENDTIARIG RSCLQQLILQ NVSKFNEYHW NQIGDVFDKL FDLTTANELF
    1701  DYDPLQQGRK SSVSHHQTTN DTSQHSDDDS NDRRENDSNI SETVERAHQE
    1751  ESSEDVGGDM VETLNGQTKL NNGNSVPTVK DELNPKPASL SIPKKTKHMK
    1801  RNESNEDIRR RINIKNSIVV KCVLQLLMIE LLNELFENED FAHCIPYKEA
    1851  IRITRLLEKS YEFSRDFNED YGLRTRLVEA RVVDKIPNLL KQETSAAAVL
    1901  LDIMFQLYLN DDEKKADLIT RLITICIQVV EGYVSLDDRT MERSINAWRS
    1951  VIVEILQGYY EFDDEDFRLY CPAMYALVIQ ILDKSVPTEL RHAIKQFLSR
    2001  VGELYLSTD*

external links for Sec7p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Sec7p

1)	Novick P, et al. (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21(1):205-15
2)	Achstetter T, et al. (1988) SEC7 encodes an unusual, high molecular weight protein required for membrane traffic from the yeast Golgi apparatus. J Biol Chem 263(24):11711-7
3)	Wolf J, et al. (1998) An N-end rule destabilization mutant reveals pre-Golgi requirements for Sec7p in yeast membrane traffic. Biochem Biophys Res Commun 243(1):191-8
4)	Franzusoff A, et al. (1991) Localization of components involved in protein transport and processing through the yeast Golgi apparatus. J Cell Biol 112(1):27-37
5)	Novick P and Schekman R (1979) Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 76(4):1858-62
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41