TRM82/YDR165W Protein Information

Standard Name	Trm82p 1
Systematic Name	Ydr165wp
ORF Classification	Verified
Description	Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p (1, 2, 3, 4)
Name Description	Transfer RNA Methyltransferase 1

Experimental Data
Molecules/cell	5910 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	444
Molecular Weight (Da)	50,488
Isoelectric Point (pI)	6.7

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Trm82p (InterPro)
Physical Interactions	There are 14 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000069623 \| Q03774 MIPS: YDR165W NCBI: 163310991 \| 163310992 \| 32363272 \| 6320369 \| 899412 \| NP_010449.1 GenBank/EMBL/DDBJ: DAA12005.1 \| Z50046

Amino Acid Sequence (or in FASTA format)
1 MSVIHPLQNL LTSRDGSLVF AIIKNCILSF KYQSPNHWEF AGKWSDDFDK 51 IQESRNTTAK EQQGQSSENE NENKKLKSNK GDSIKRTAAK VPSPGLGAPP 101 IYSYIRNLRL TSDESRLIAC ADSDKSLLVF DVDKTSKNVL KLRKRFCFSK 151 RPNAISIAED DTTVIIADKF GDVYSIDINS IPEEKFTQEP ILGHVSMLTD 201 VHLIKDSDGH QFIITSDRDE HIKISHYPQC FIVDKWLFGH KHFVSSICCG 251 KDYLLLSAGG DDKIFAWDWK TGKNLSTFDY NSLIKPYLND QHLAPPRFQN 301 ENNDIIEFAV SKIIKSKNLP FVAFFVEATK CIIILEMSEK QKGDLALKQI 351 ITFPYNVISL SAHNDEFQVT LDNKESSGVQ KNFAKFIEYN LNENSFVVNN 401 EKSNEFDSAI IQSVQGDSNL VTKKEEIYPL YNVSSLRKHG EHYS*

Amino Acid Sequence (or in FASTA format)

       1  MSVIHPLQNL LTSRDGSLVF AIIKNCILSF KYQSPNHWEF AGKWSDDFDK
      51  IQESRNTTAK EQQGQSSENE NENKKLKSNK GDSIKRTAAK VPSPGLGAPP
     101  IYSYIRNLRL TSDESRLIAC ADSDKSLLVF DVDKTSKNVL KLRKRFCFSK
     151  RPNAISIAED DTTVIIADKF GDVYSIDINS IPEEKFTQEP ILGHVSMLTD
     201  VHLIKDSDGH QFIITSDRDE HIKISHYPQC FIVDKWLFGH KHFVSSICCG
     251  KDYLLLSAGG DDKIFAWDWK TGKNLSTFDY NSLIKPYLND QHLAPPRFQN
     301  ENNDIIEFAV SKIIKSKNLP FVAFFVEATK CIIILEMSEK QKGDLALKQI
     351  ITFPYNVISL SAHNDEFQVT LDNKESSGVQ KNFAKFIEYN LNENSFVVNN
     401  EKSNEFDSAI IQSVQGDSNL VTKKEEIYPL YNVSSLRKHG EHYS*

external links for Trm82p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Trm82p

1)	Alexandrov A, et al. (2002) Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA. RNA 8(10):1253-66
2)	Matsumoto K, et al. (2007) RNA recognition mechanism of eukaryote tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex). FEBS Lett 581(8):1599-604
3)	Matsumoto K, et al. (2008) Production of yeast tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex) in a wheat germ cell-free translation system. J Biotechnol 133(4):453-60
4)	Leulliot N, et al. (2008) Structure of the Yeast tRNA m7G Methylation Complex. Structure 16(1):52-61
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41