GIR2/YDR152W Protein Information

Standard Name	Gir2p
Systematic Name	Ydr152wp
ORF Classification	Verified
Description	Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein (1, 2, 3, 4)
Name Description	Genetically Interacts with Ribosomal genes 3

Experimental Data
Molecules/cell	10300 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	265
Molecular Weight (Da)	31,030
Isoelectric Point (pI)	4.33

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gir2p (InterPro)
Physical Interactions	There are 28 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000694E0 \| Q03768 MIPS: YDR152W NCBI: 30913066 \| 398365865 \| 45269255 \| 899401 \| NP_010436.3 GenBank/EMBL/DDBJ: DAA11994.1 \| AY557681 \| Z50046

Amino Acid Sequence (or in FASTA format)
1 MDYKEEQKQE LEVLESIYPD ELRIINDEYP KIKFEVAIKL ELDTGDSTSV 51 LTKEHTIIAE FKLPENYPDE PCLISLEAQE VALNDNEEDN EEDEDEVEYD 101 DHGNKVLKKF ENLPDLISFK GYLPELTVQL ESQIETDMLL GMQMCFALIS 151 SIKERCEQWY SEQLNKLEKQ YELEAQEREK KEQAKFHGTK VTRESYLEWR 201 SKFRQELKLD ERDQVRRMKA HHGKLTGKQM FEQGVVGTGD EYMEEDDASV 251 DDVAKGLAKT EIANQ*

Amino Acid Sequence (or in FASTA format)

       1  MDYKEEQKQE LEVLESIYPD ELRIINDEYP KIKFEVAIKL ELDTGDSTSV
      51  LTKEHTIIAE FKLPENYPDE PCLISLEAQE VALNDNEEDN EEDEDEVEYD
     101  DHGNKVLKKF ENLPDLISFK GYLPELTVQL ESQIETDMLL GMQMCFALIS
     151  SIKERCEQWY SEQLNKLEKQ YELEAQEREK KEQAKFHGTK VTRESYLEWR
     201  SKFRQELKLD ERDQVRRMKA HHGKLTGKQM FEQGVVGTGD EYMEEDDASV
     251  DDVAKGLAKT EIANQ*

external links for Gir2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Gir2p

1)	Alves VS, et al. (2004) Biophysical characterization of Gir2, a highly acidic protein of Saccharomyces cerevisiae with anomalous electrophoretic behavior. Biochem Biophys Res Commun 314(1):229-34
2)	Alves VS and Castilho BA (2005) Gir2 is an intrinsically unstructured protein that is present in Saccharomyces cerevisiae as a group of heterogeneously electrophoretic migrating forms. Biochem Biophys Res Commun 332(2):450-5
3)	Wout PK, et al. (2009) Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on Polyribosomes with Gcn1. Eukaryot Cell 8(7):1061-71
4)	Daugeron MC, et al. (2011) The highly conserved eukaryotic DRG factors are required for efficient translation in a manner redundant with the putative RNA helicase Slh1. Nucleic Acids Res 39(6):2221-33
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41