EKI1/YDR147W Protein Information

Standard Name	Eki1p 1
Systematic Name	Ydr147wp
ORF Classification	Verified
Description	Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication (1, 2, 3)
Name Description	Ethanolamine KInase 1

Experimental Data
Molecules/cell	3420 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	534
Molecular Weight (Da)	61,656
Isoelectric Point (pI)	5.69

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Eki1p (InterPro)
Physical Interactions	There are 5 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000129E30 \| Q03764 MIPS: YDR147W NCBI: 398365849 \| 6685418 \| 899397 \| NP_010431.3 GenBank/EMBL/DDBJ: DAA11990.1 \| Z50046
External Classifications	EC: 2.7.1.82 [Ethanolamine kinase]

Amino Acid Sequence (or in FASTA format)
1 MYTNYSLTSS DAMPRTYLVG TASPEMSKKK RQSANCDKPT RRVIHIIDTN 51 EHSEVDLKNE LPITCTNEDG EMTSSSWTSQ TANDFLKLAY VNAKLDPSLP 101 SQYFKQDIIN VLQSLEIPGW SVPGSKESSL NKNLLTLTQI KGALTNVIYK 151 IHYPNLPPLL MRIFGDSIDS VIDREYELKV IARLSFYDLG PKLEGFFENG 201 RFEKYIEGSR TSTQADFIDR DTSIKIAKKL KELHCTVPLT HKEITDQPSC 251 WTTFDQWIKL IDSHKEWVSN NVNISENLRC SSWNFFLKSF KNYKRWLYND 301 SAFTSKLLRE DDKDSMINSG LKMVFCHNDL QHGNLLFKSK GKDDISVGDL 351 TIIDFEYAGP NPVVFDLSNH LNEWMQDYND VQSFKSHIDK YPKEEDILVF 401 AQSYINHMNE NHVKIASQEV RILYNLIIEW RPCTQLFWCL WALLQSGRLP 451 QRPLIEGEKL MSEKAGLGDE THLMEHKNKE NGKYDCSEDD SFNYLGFCKE 501 KMSVFWGDLI TLGVIDKDCP DIGKTDYLDT KLIF*

Amino Acid Sequence (or in FASTA format)

       1  MYTNYSLTSS DAMPRTYLVG TASPEMSKKK RQSANCDKPT RRVIHIIDTN
      51  EHSEVDLKNE LPITCTNEDG EMTSSSWTSQ TANDFLKLAY VNAKLDPSLP
     101  SQYFKQDIIN VLQSLEIPGW SVPGSKESSL NKNLLTLTQI KGALTNVIYK
     151  IHYPNLPPLL MRIFGDSIDS VIDREYELKV IARLSFYDLG PKLEGFFENG
     201  RFEKYIEGSR TSTQADFIDR DTSIKIAKKL KELHCTVPLT HKEITDQPSC
     251  WTTFDQWIKL IDSHKEWVSN NVNISENLRC SSWNFFLKSF KNYKRWLYND
     301  SAFTSKLLRE DDKDSMINSG LKMVFCHNDL QHGNLLFKSK GKDDISVGDL
     351  TIIDFEYAGP NPVVFDLSNH LNEWMQDYND VQSFKSHIDK YPKEEDILVF
     401  AQSYINHMNE NHVKIASQEV RILYNLIIEW RPCTQLFWCL WALLQSGRLP
     451  QRPLIEGEKL MSEKAGLGDE THLMEHKNKE NGKYDCSEDD SFNYLGFCKE
     501  KMSVFWGDLI TLGVIDKDCP DIGKTDYLDT KLIF*

external links for Eki1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Eki1p

1)	Kim K, et al. (1999) Isolation and characterization of the Saccharomyces cerevisiae EKI1 gene encoding ethanolamine kinase. J Biol Chem 274(21):14857-66
2)	Birner R, et al. (2001) Roles of phosphatidylethanolamine and of its several biosynthetic pathways in Saccharomyces cerevisiae. Mol Biol Cell 12(4):997-1007
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41