SWI5/YDR146C Protein Information

Standard Name	Swi5p 1, 2
Systematic Name	Ydr146cp
ORF Classification	Verified
Description	Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7, 8, 9)
Name Description	SWItching deficient 1

Experimental Data
Molecules/cell	688 10

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	709
Molecular Weight (Da)	79,774
Isoelectric Point (pI)	9.11

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Swi5p (InterPro)
Physical Interactions	There are 56 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001362A0 \| P08153 MIPS: YDR146C NCBI: 135077 \| 159163679 \| 449122447 \| 4596 \| 51830243 \| 6320350 \| 899396 \| NP_010430.1 GenBank/EMBL/DDBJ: DAA11989.1 \| AY723778 \| X06978 \| Z50046

Amino Acid Sequence (or in FASTA format)
1 MDTSNSWFDA SKVQSLNFDL QTNSYYSNAR GSDPSSYAIE GEYKTLATDD 51 LGNILNLNYG ETNEVIMNEI NDLNLPLGPL SDEKSVKVST FSELIGNDWQ 101 SMNFDLENNS REVTLNATSL LNENRLNQDS GMTVYQKTMS DKPHDEKKIS 151 MADNLLSTIN KSEINKGFDR NLGELLLQQQ QELREQLRAQ QEANKKLELE 201 LKQTQYKQQQ LQATLENSDG PQFLSPKRKI SPASENVEDV YANSLSPMIS 251 PPMSNTSFTG SPSRRNNRQK YCLQRKNSSG TVGPLCFQEL NEGFNDSLIS 301 PKKIRSNPNE NLSSKTKFIT PFTPKSRVSS ATSNSANITP NNLRLDFKIN 351 VEDQESEYSE KPLGLGIELL GKPGPSPTKS VSLKSASVDI MPTIPGSVNN 401 TPSVNKVSLS SSYIDQYTPR GKQLHFSSIS ENALGINAAT PHLKPPSQQA 451 RHREGVFNDL DPNVLTKNTD NEGDDNEENE PESRFVISET PSPVLKSQSK 501 YEGRSPQFGT HIKEINTYTT NSPSKITRKL TTLPRGSIDK YVKEMPDKTF 551 ECLFPGCTKT FKRRYNIRSH IQTHLEDRPY SCDHPGCDKA FVRNHDLIRH 601 KKSHQEKAYA CPCGKKFNRE DALVVHRSRM ICSGGKKYEN VVIKRSPRKR 651 GRPRKDGTSS VSSSPIKENI NKDHNGQLMF KLEDQLRRER SYDGNGTGIM 701 VSPMKTNQR*

Amino Acid Sequence (or in FASTA format)

       1  MDTSNSWFDA SKVQSLNFDL QTNSYYSNAR GSDPSSYAIE GEYKTLATDD
      51  LGNILNLNYG ETNEVIMNEI NDLNLPLGPL SDEKSVKVST FSELIGNDWQ
     101  SMNFDLENNS REVTLNATSL LNENRLNQDS GMTVYQKTMS DKPHDEKKIS
     151  MADNLLSTIN KSEINKGFDR NLGELLLQQQ QELREQLRAQ QEANKKLELE
     201  LKQTQYKQQQ LQATLENSDG PQFLSPKRKI SPASENVEDV YANSLSPMIS
     251  PPMSNTSFTG SPSRRNNRQK YCLQRKNSSG TVGPLCFQEL NEGFNDSLIS
     301  PKKIRSNPNE NLSSKTKFIT PFTPKSRVSS ATSNSANITP NNLRLDFKIN
     351  VEDQESEYSE KPLGLGIELL GKPGPSPTKS VSLKSASVDI MPTIPGSVNN
     401  TPSVNKVSLS SSYIDQYTPR GKQLHFSSIS ENALGINAAT PHLKPPSQQA
     451  RHREGVFNDL DPNVLTKNTD NEGDDNEENE PESRFVISET PSPVLKSQSK
     501  YEGRSPQFGT HIKEINTYTT NSPSKITRKL TTLPRGSIDK YVKEMPDKTF
     551  ECLFPGCTKT FKRRYNIRSH IQTHLEDRPY SCDHPGCDKA FVRNHDLIRH
     601  KKSHQEKAYA CPCGKKFNRE DALVVHRSRM ICSGGKKYEN VVIKRSPRKR
     651  GRPRKDGTSS VSSSPIKENI NKDHNGQLMF KLEDQLRRER SYDGNGTGIM
     701  VSPMKTNQR*

external links for Swi5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Swi5p

1)	Stern M, et al. (1984) Five SWI genes are required for expression of the HO gene in yeast. J Mol Biol 178(4):853-68
2)	Stillman, D. (1993) Personal Communication, Mortimer Map Edition 12
3)	Moll T, et al. (1991) The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5. Cell 66(4):743-58
4)	Cosma MP, et al. (1999) Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter. Cell 97(3):299-311
5)	McBride HJ, et al. (1999) Distinct regions of the Swi5 and Ace2 transcription factors are required for specific gene activation. J Biol Chem 274(30):21029-36
6)	Dohrmann PR, et al. (1992) Parallel pathways of gene regulation: homologous regulators SWI5 and ACE2 differentially control transcription of HO and chitinase. Genes Dev 6(1):93-104
7)	Bhoite LT, et al. (2001) The Swi5 activator recruits the Mediator complex to the HO promoter without RNA polymerase II. Genes Dev 15(18):2457-69
8)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
9)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
10)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41