YCF1/YDR135C Protein Information Help

Standard Name Ycf1p 1
Systematic Name Ydr135cp
ORF Classification Verified
Description Vacuolar glutathione S-conjugate transporter; member of ATP-binding cassette family; ATPase activity required to support vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; has role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR (1, 2, 3, 4, 5, 6, 7)
Name Description Yeast Cadmium Factor 1
Experimental Data
Molecules/cell 396 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,515
Molecular Weight (Da) 171,120
Isoelectric Point (pI) 8.49

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ycf1p (InterPro)
Transmembrane Domains There are 14 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 26 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013A67B | P39109
MIPS: YDR135C
NCBI: 398365779 | 556465 | 665668 | 6920083 | NP_010419.3
GenBank/EMBL/DDBJ: DAA11979.1 | L35237 | Z48179
External Classifications TC: 3.A.1.208 [The Conjugate Transporter-2 (CT2) Family]
Amino Acid Sequence (or in FASTA format)
 
       1  MAGNLVSWAC KLCRSPEGFG PISFYGDFTQ CFIDGVILNL SAIFMITFGI
      51  RDLVNLCKKK HSGIKYRRNW IIVSRMALVL LEIAFVSLAS LNISKEEAEN
     101  FTIVSQYAST MLSLFVALAL HWIEYDRSVV ANTVLLFYWL FETFGNFAKL
     151  INILIRHTYE GIWYSGQTGF ILTLFQVITC ASILLLEALP KKPLMPHQHI
     201  HQTLTRRKPN PYDSANIFSR ITFSWMSGLM KTGYEKYLVE ADLYKLPRNF
     251  SSEELSQKLE KNWENELKQK SNPSLSWAIC RTFGSKMLLA AFFKAIHDVL
     301  AFTQPQLLRI LIKFVTDYNS ERQDDHSSLQ GFENNHPQKL PIVRGFLIAF
     351  AMFLVGFTQT SVLHQYFLNV FNTGMYIKSA LTALIYQKSL VLSNEASGLS
     401  STGDIVNLMS VDVQKLQDLT QWLNLIWSGP FQIIICLYSL YKLLGNSMWV
     451  GVIILVIMMP LNSFLMRIQK KLQKSQMKYK DERTRVISEI LNNIKSLKLY
     501  AWEKPYREKL EEVRNNKELK NLTKLGCYMA VTSFQFNIVP FLVSCCTFAV
     551  FVYTEDRALT TDLVFPALTL FNLLSFPLMI IPMVLNSFIE ASVSIGRLFT
     601  FFTNEELQPD SVQRLPKVKN IGDVAINIGD DATFLWQRKP EYKVALKNIN
     651  FQAKKGNLTC IVGKVGSGKT ALLSCMLGDL FRVKGFATVH GSVAYVSQVP
     701  WIMNGTVKEN ILFGHRYDAE FYEKTIKACA LTIDLAILMD GDKTLVGEKG
     751  ISLSGGQKAR LSLARAVYAR ADTYLLDDPL AAVDEHVARH LIEHVLGPNG
     801  LLHTKTKVLA TNKVSALSIA DSIALLDNGE ITQQGTYDEI TKDADSPLWK
     851  LLNNYGKKNN GKSNEFGDSS ESSVRESSIP VEGELEQLQK LNDLDFGNSD
     901  AISLRRASDA TLGSIDFGDD ENIAKREHRE QGKVKWNIYL EYAKACNPKS
     951  VCVFILFIVI SMFLSVMGNV WLKHWSEVNS RYGSNPNAAR YLAIYFALGI
    1001  GSALATLIQT IVLWVFCTIH ASKYLHNLMT NSVLRAPMTF FETTPIGRIL
    1051  NRFSNDIYKV DALLGRTFSQ FFVNAVKVTF TITVICATTW QFIFIIIPLS
    1101  VFYIYYQQYY LRTSRELRRL DSITRSPIYS HFQETLGGLA TVRGYSQQKR
    1151  FSHINQCRID NNMSAFYPSI NANRWLAYRL ELIGSIIILG AATLSVFRLK
    1201  QGTLTAGMVG LSLSYALQIT QTLNWIVRMT VEVETNIVSV ERIKEYADLK
    1251  SEAPLIVEGH RPPKEWPSQG DIKFNNYSTR YRPELDLVLK HINIHIKPNE
    1301  KVGIVGRTGA GKSSLTLALF RMIEASEGNI VIDNIAINEI GLYDLRHKLS
    1351  IIPQDSQVFE GTVRENIDPI NQYTDEAIWR ALELSHLKEH VLSMSNDGLD
    1401  AQLTEGGGNL SVGQRQLLCL ARAMLVPSKI LVLDEATAAV DVETDKVVQE
    1451  TIRTAFKDRT ILTIAHRLNT IMDSDRIIVL DNGKVAEFDS PGQLLSDNKS
    1501  LFYSLCMEAG LVNEN*

external links for Ycf1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome TCDB
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Ycf1p
1) Szczypka MS, et al.  (1994) A yeast metal resistance protein similar to human cystic fibrosis transmembrane conductance regulator (CFTR) and multidrug resistance-associated protein. J Biol Chem 269(36):22853-7
2) Petrovic S, et al.  (2000) The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae. Yeast 16(6):561-71
3) Li ZS, et al.  (1996) The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump. J Biol Chem 271(11):6509-17
4) Li ZS, et al.  (1997) A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae: YCF1-catalyzed transport of bis(glutathionato)cadmium. Proc Natl Acad Sci U S A 94(1):42-7
5) Gueldry O, et al.  (2003) Ycf1p-dependent Hg(II) detoxification in Saccharomyces cerevisiae. Eur J Biochem 270(11):2486-96
6) Lazard M, et al.  (2011) Selenodiglutathione uptake by the Saccharomyces cerevisiae vacuolar ATP-binding cassette transporter Ycf1p. FEBS J 278(21):4112-21
7) Sasser TL, et al.  (2013) The Yeast ABC Transporter Ycf1p Enhances the Recruitment of the Soluble SNARE Vam7p to Vacuoles for Efficient Membrane Fusion. J Biol Chem ()
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41