APC4/YDR118W Protein Information Help

Standard Name Apc4p 1
Systematic Name Ydr118wp
ORF Classification Verified
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3, 4)
Name Description Anaphase Promoting Complex 1
Experimental Data
Molecules/cell 1330 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 652
Molecular Weight (Da) 75,255
Isoelectric Point (pI) 5.64

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Apc4p (InterPro)
Physical Interactions There are 106 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000006A55B | Q04601
MIPS: YDR118W
NCBI: 398365699 | 55976289 | 747892 | NP_010403.3
GenBank/EMBL/DDBJ: DAA11963.1 | Z48758
Amino Acid Sequence (or in FASTA format)
 
       1  MSSPINDYFI DYNPLFPIFA TRIAKGLAIY RVSDHARLAV IPIRNINLVA
      51  NYDWDTTTGK FLSIFFKDGT IRIHDIFKDG RLVSFLRIPS TKISKGIWDR
     101  IPLRYEPNNR DFACNIIDDL PKLIRFVKDS KRINIVPYTQ PNSLWRGPDE
     151  DDLDSNEKLD VHVVFNEGND KITVFFNGDY AVFLSVDNIE NENSLKSIIK
     201  VQDGFYQCFY EDGTVQTLNL GPLLQSKSSV NLLNYIMVIK ELIGYMLTHL
     251  EFINRELATP YLDFVKRLCD EAYGYGKLKS ELEALFLLGE ISCDLEDWLC
     301  NSVGEKNFKR WKYLGCEAYQ KTVQILTLIF VPACERIIIY VEKLRAILQA
     351  FSIQNKLSYT SDLTAVEVLL KSSQKLLTMT LNSIIGLGRD ETLFEKFFIW
     401  FNDRLHEALD EDYKLKFQFE DDLYFGYDLL SYFDRILSKK GTEPSSIIDV
     451  KLYRDLINSM SDMEKDIAQS NVNSHIQQHI LVDLKTDVFA QKYPSSQINL
     501  LDAIKLPKHN YIVYLIQVTK HNSAQEPFSE ENKKKLYIGT LKDENLGIIS
     551  KESSVKIPAL FKSYRLSSTR FVPNRVHSLL RDIGLSDSNY HSSHVTDYRG
     601  ENYENEEDDG TIAIPAYIRE NRENDDFIAC TAKVSVDGRS ASLVFPKEKQ
     651  NV*

external links for Apc4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Apc4p
1) Zachariae W, et al.  (1998) Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science 279(5354):1216-9
2) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
3) Buschhorn BA, et al.  (2011) Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1. Nat Struct Mol Biol 18(1):6-13
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41