FOB1/YDR110W Protein Information

Standard Name	Fob1p 1
Systematic Name	Ydr110wp
Alias	Hrm1p 2
ORF Classification	Verified
Description	Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases (1, 2, 3, 4, 5)
Name Description	FOrk Blocking less 1

Experimental Data
Molecules/cell	1510 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	566
Molecular Weight (Da)	65,318
Isoelectric Point (pI)	6.12

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Fob1p (InterPro)
Physical Interactions	There are 45 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001682EA \| O13329 MIPS: YDR110W NCBI: 2331297 \| 308153446 \| 3122096 \| 6320315 \| 747885 \| NP_010395.1 \| NM_001180418.1 GenBank/EMBL/DDBJ: DAA11956.1 \| AF013245 \| Z48758

Amino Acid Sequence (or in FASTA format)
1 MTKPRYNDVL FDDDDSVPSE SVTRKSQRRK ATSPGESRES SKDRLLILPS 51 MGESYTEYVD SYLNLELLER GERETPIFLE SLTRQLTQKI YELIKTKSLT 101 ADTLQQISDK YDGVVAENKL LFLQRQYYVD DEGNVRDGRN NDKIYCEPKH 151 VYDMVMATHL MNKHLRGKTL HSFLFSHFAN ISHAIIDWVQ QFCSKCNKKG 201 KIKPLKEYKR PDMYDKLLPM ERIHIEVFEP FNGEAIEGKY SYVLLCRDYR 251 SSFMWLLPLK STKFKHLIPV VSSLFLTFAR VPIFVTSSTL DKDDLYDICE 301 EIASKYGLRI GLGLKSSARF HTGGILCIQY ALNSYKKECL ADWGKCLRYG 351 PYRFNRRRNK RTKRKPVQVL LSEVPGHNAK FETKRERVIE NTYSRNMFKM 401 AGGKGLIYLE DVNTFALANE ADNSCNNNGI LHNNNIGNDN FEEEVQKQFD 451 LTEKNYIDEY DDLAHDSSEG EFEPNTLTPE EKPPHNVDED RIESTGVAAP 501 MQGTEEPEKG DQKESDGASQ VDQSVEITRP ETSYYQTLES PSTKRQKLDQ 551 QGNGDQTRDF GTSMEL*

Amino Acid Sequence (or in FASTA format)

       1  MTKPRYNDVL FDDDDSVPSE SVTRKSQRRK ATSPGESRES SKDRLLILPS
      51  MGESYTEYVD SYLNLELLER GERETPIFLE SLTRQLTQKI YELIKTKSLT
     101  ADTLQQISDK YDGVVAENKL LFLQRQYYVD DEGNVRDGRN NDKIYCEPKH
     151  VYDMVMATHL MNKHLRGKTL HSFLFSHFAN ISHAIIDWVQ QFCSKCNKKG
     201  KIKPLKEYKR PDMYDKLLPM ERIHIEVFEP FNGEAIEGKY SYVLLCRDYR
     251  SSFMWLLPLK STKFKHLIPV VSSLFLTFAR VPIFVTSSTL DKDDLYDICE
     301  EIASKYGLRI GLGLKSSARF HTGGILCIQY ALNSYKKECL ADWGKCLRYG
     351  PYRFNRRRNK RTKRKPVQVL LSEVPGHNAK FETKRERVIE NTYSRNMFKM
     401  AGGKGLIYLE DVNTFALANE ADNSCNNNGI LHNNNIGNDN FEEEVQKQFD
     451  LTEKNYIDEY DDLAHDSSEG EFEPNTLTPE EKPPHNVDED RIESTGVAAP
     501  MQGTEEPEKG DQKESDGASQ VDQSVEITRP ETSYYQTLES PSTKRQKLDQ
     551  QGNGDQTRDF GTSMEL*

external links for Fob1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Fob1p

1)	Kobayashi T and Horiuchi T (1996) A yeast gene product, Fob1 protein, required for both replication fork blocking and recombinational hotspot activities. Genes Cells 1(5):465-74
2)	Defossez PA, et al. (1999) Elimination of replication block protein Fob1 extends the life span of yeast mother cells. Mol Cell 3(4):447-55
3)	Dlakic M (2002) A model of the replication fork blocking protein Fob1p based on the catalytic core domain of retroviral integrases. Protein Sci 11(5):1274-7
4)	Kobayashi T (2003) The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork. Mol Cell Biol 23(24):9178-88
5)	Johzuka K and Horiuchi T (2009) The cis element and factors required for condensin recruitment to chromosomes. Mol Cell 34(1):26-35
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41