FOB1/YDR110W Protein Information Help

Standard Name Fob1p 1
Systematic Name Ydr110wp
Alias Hrm1p 2
ORF Classification Verified
Description Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to replication fork barrier (RFB), and rDNA repeat segregation; related to retroviral integrases (1, 2, 3, 4, 5, 6)
Name Description FOrk Blocking less 1
Experimental Data
Molecules/cell 1510 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 566
Molecular Weight (Da) 65,318
Isoelectric Point (pI) 6.12

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Fob1p (InterPro)
Physical Interactions There are 48 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001682EA | O13329
MIPS: YDR110W
NCBI: 2331297 | 308153446 | 3122096 | 6320315 | 747885 | NP_010395.1 | NM_001180418.1
GenBank/EMBL/DDBJ: AAB66897.1 | CAA88664.1 | DAA11956.1 | AF013245 | Z48758
Amino Acid Sequence (or in FASTA format)
       1  MTKPRYNDVL FDDDDSVPSE SVTRKSQRRK ATSPGESRES SKDRLLILPS
      51  MGESYTEYVD SYLNLELLER GERETPIFLE SLTRQLTQKI YELIKTKSLT
     101  ADTLQQISDK YDGVVAENKL LFLQRQYYVD DEGNVRDGRN NDKIYCEPKH
     151  VYDMVMATHL MNKHLRGKTL HSFLFSHFAN ISHAIIDWVQ QFCSKCNKKG
     201  KIKPLKEYKR PDMYDKLLPM ERIHIEVFEP FNGEAIEGKY SYVLLCRDYR
     251  SSFMWLLPLK STKFKHLIPV VSSLFLTFAR VPIFVTSSTL DKDDLYDICE
     301  EIASKYGLRI GLGLKSSARF HTGGILCIQY ALNSYKKECL ADWGKCLRYG
     351  PYRFNRRRNK RTKRKPVQVL LSEVPGHNAK FETKRERVIE NTYSRNMFKM
     401  AGGKGLIYLE DVNTFALANE ADNSCNNNGI LHNNNIGNDN FEEEVQKQFD
     451  LTEKNYIDEY DDLAHDSSEG EFEPNTLTPE EKPPHNVDED RIESTGVAAP
     501  MQGTEEPEKG DQKESDGASQ VDQSVEITRP ETSYYQTLES PSTKRQKLDQ
     551  QGNGDQTRDF GTSMEL*                                    

external links for Fob1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB CYC2008 (complexes) MIPS YeastGFP
YOGY Complexome Pfam domains YeastRC Public Image Repository

DIP


GeneMANIA


IMP


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Fob1p
1) Kobayashi T and Horiuchi T  (1996) A yeast gene product, Fob1 protein, required for both replication fork blocking and recombinational hotspot activities. Genes Cells 1(5):465-74
2) Defossez PA, et al.  (1999) Elimination of replication block protein Fob1 extends the life span of yeast mother cells. Mol Cell 3(4):447-55
3) Dlakic M  (2002) A model of the replication fork blocking protein Fob1p based on the catalytic core domain of retroviral integrases. Protein Sci 11(5):1274-7
4) Kobayashi T  (2003) The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork. Mol Cell Biol 23(24):9178-88
5) Machin F, et al.  (2006) Transcription of ribosomal genes can cause nondisjunction. J Cell Biol 173(6):893-903
6) Johzuka K and Horiuchi T  (2009) The cis element and factors required for condensin recruitment to chromosomes. Mol Cell 34(1):26-35
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41