TRS85/YDR108W Protein Information Help

Standard Name Trs85p 1
Systematic Name Ydr108wp
Alias Mum1p 2 , Gsg1p 3
ORF Classification Verified
Description Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role (2, 3, 4, 5, 6)
Name Description TRapp Subunit 1
Experimental Data
Molecules/cell 8760 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 698
Molecular Weight (Da) 80,494
Isoelectric Point (pI) 6.38

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Trs85p (InterPro)
Physical Interactions There are 56 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052E15 | P46944
MIPS: YDR108W
NCBI: 20178340 | 398365643 | 747883 | 849131 | NP_010393.3
GenBank/EMBL/DDBJ: DAA11954.1 | U26674 | Z48758
Amino Acid Sequence (or in FASTA format)
 
       1  MVFSYEHYMN LLFHLDNSKE TVPPEIAKRI ISNAIAPVIT VTSTPLFDKH
      51  IQETYKVDSL YMLLRFFGGC VSDRDQANEA KVGQHEHEVC DASDSTDSIP
     101  KNKNLEVPNL SKKGSRSRSN SLFQRDSTQS QYIRFTRPLG DLIETRDAND
     151  MLFNYHSLEV FLDNYLKLVA ANTDEMVPHN LLKKSIYHSF FSLAISSTNN
     201  LSPYETFNHP ILSLIALDIS NGEVYEDARD LLVNFKNLNH NTENFPIFMN
     251  TNEMLPVFLL CYNDDSQEEF EKCQALAKKL KKQLFVESIL LALWKDSFIY
     301  DENSVIQLHQ PVMSSLEEIL FFLQAPTQTT LSLALINSIY DMLDYLVYDL
     351  MIPFMKRKVS FWEETILQPR KSLFNGAKFF KKFMNKNPVN GNHQHNSLTR
     401  DSQGNEYFAS SSSEFLMRKL ADWSMMLSDF KTAYSTYESL MDDLDAFPKY
     451  LASCIEWCAV SLLMGAQSIV TVKMIKNDIN PLIERALATY ENCSRIQRGK
     501  GKESNSLDVT EPVRSYETRC MILASELFLS LSNTWTSTPY AIQYLETILD
     551  ECKLGPCSQI MVWERLSDCY NLRVDPRIKH RVGAMKKDAK DTEDLRGEHK
     601  YSTDHFTDED ILSEGLTRRR KAAFFRLIAA KKWAEQKQWR QVSWCLKDIE
     651  STYSEIKFLH GNGLILSKLK NQLNLKDVDS APRPSEKNLT RTSVSFIG*

external links for Trs85p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Trs85p
1) Sacher M, et al.  (2000) Identification and characterization of five new subunits of TRAPP. Eur J Cell Biol 79(2):71-80
2) Engebrecht J, et al.  (1998) Yeast meiotic mutants proficient for the induction of ectopic recombination. Genetics 148(2):581-98
3) Kaytor MD and Livingston DM  (1995) GSG1, a yeast gene required for sporulation. Yeast 11(12):1147-55
4) Meiling-Wesse K, et al.  (2005) Trs85 (Gsg1), a component of the TRAPP complexes, is required for the organization of the preautophagosomal structure during selective autophagy via the Cvt pathway. J Biol Chem 280(39):33669-78
5) Nazarko TY, et al.  (2005) Trs85 is required for macroautophagy, pexophagy and cytoplasm to vacuole targeting in Yarrowia lipolytica and Saccharomyces cerevisiae. Autophagy 1(1):37-45
6) Lynch-Day MA, et al.  (2010) Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy. Proc Natl Acad Sci U S A 107(17):7811-6
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41