GRX3/YDR098C Protein Information

Standard Name	Grx3p 1
Systematic Name	Ydr098cp
ORF Classification	Verified
Description	Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36; GRX3 has a paralog, GRX4, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	GlutaRedoXin 1

Experimental Data
Molecules/cell	11000 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	285
Molecular Weight (Da)	32,481
Isoelectric Point (pI)	4.37

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Grx3p (InterPro)
Physical Interactions	There are 35 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013A8DF \| Q03835 MIPS: YDR098C NCBI: 197725194 \| 197725195 \| 3025094 \| 398365573 \| 633632 \| NP_010383.3 GenBank/EMBL/DDBJ: DAA11943.1 \| Z47746

Amino Acid Sequence (or in FASTA format)
1 MCSFQVPSAF SFNYTSYCYK RHQARYYTAA KLFQEMPVIE INDQEQFTYL 51 TTTAAGDKLI VLYFHTSWAE PCKALKQVFE AISNEPSNSN VSFLSIDADE 101 NSEISELFEI SAVPYFIIIH KGTILKELSG ADPKEYVSLL EDCKNSVNSG 151 SSQTHTMENA NVNEGSHNDE DDDDEEEEEE TEEQINARLT KLVNAAPVML 201 FMKGSPSEPK CGFSRQLVGI LREHQVRFGF FDILRDESVR QNLKKFSEWP 251 TFPQLYINGE FQGGLDIIKE SLEEDPDFLQ HALQS*

Amino Acid Sequence (or in FASTA format)

       1  MCSFQVPSAF SFNYTSYCYK RHQARYYTAA KLFQEMPVIE INDQEQFTYL
      51  TTTAAGDKLI VLYFHTSWAE PCKALKQVFE AISNEPSNSN VSFLSIDADE
     101  NSEISELFEI SAVPYFIIIH KGTILKELSG ADPKEYVSLL EDCKNSVNSG
     151  SSQTHTMENA NVNEGSHNDE DDDDEEEEEE TEEQINARLT KLVNAAPVML
     201  FMKGSPSEPK CGFSRQLVGI LREHQVRFGF FDILRDESVR QNLKKFSEWP
     251  TFPQLYINGE FQGGLDIIKE SLEEDPDFLQ HALQS*

external links for Grx3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Grx3p

1)	Rodriguez-Manzaneque MT, et al. (1999) Grx5 glutaredoxin plays a central role in protection against protein oxidative damage in Saccharomyces cerevisiae. Mol Cell Biol 19(12):8180-90
2)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3)	Li H, et al. (2009) The yeast iron regulatory proteins Grx3/4 and Fra2 form heterodimeric complexes containing a [2Fe-2S] cluster with cysteinyl and histidyl ligation. Biochemistry 48(40):9569-81
4)	Dingra NN (2005) Characterization of the Role of Glutaredoxin 3 and its Binding Partners in Maintaining Iron Homeostasis in Saccharomyces cerevisiae ()
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41