GIS1/YDR096W Protein Information

Standard Name	Gis1p
Systematic Name	Ydr096wp
ORF Classification	Verified
Description	Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8)
Name Description	GIg1-2 Suppressor 3

Experimental Data
Molecules/cell	432 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	894
Molecular Weight (Da)	99,480
Isoelectric Point (pI)	7.49

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gis1p (InterPro)
Physical Interactions	There are 26 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006B696 \| Q03833 MIPS: YDR096W NCBI: 51315832 \| 6320301 \| 633630 \| NP_010381.1 GenBank/EMBL/DDBJ: DAA11941.1 \| Z47746

Amino Acid Sequence (or in FASTA format)
1 MEIKPVEVID GVPVFKPSMM EFANFQYFID EITKFGIENG IVKVIPPKEW 51 LELLEGSPPA ESLKTIQLDS PIQQQAKRWD KHENGVFSIE NEYDNKSYNL 101 TQWKNLAESL DSRISQGDFN DKTLKENCRV DSQQDCYDLA QLQILESDFW 151 KTIAFSKPFY AVDENSSIFP YDLTLWNLNN LPDSINSSNR RLLTGQSKCI 201 FPWHLDEQNK CSINYLHFGA PKQWYSIPSA NTDQFLKILS KEPSSNKENC 251 PAFIRHQNII TSPDFLRKNN IKFNRVVQFQ HEFIITFPYC MYSGFNYGYN 301 FGESIEFILD QQAVVRKQPL KCGCGNKKEE RKSGPFSNLS YDSNESEQRG 351 SITDNDNDLF QKVRSFDELL NHSSQELQNL EDNKNPLFSN INMNRPQSSS 401 LRSTTPNGVN QFLNMNQTTI SRISSPLLSR MMDLSNIVEP TLDDPGSKFK 451 RKVLTPQLPQ MNIPSNSSNF GTPSLTNTNS LLSNITATST NPSTTTNGSQ 501 NHNNVNANGI NTSAAASINN NISSTNNSAN NSSSNNNVST VPSSMMHSST 551 LNGTSGLGGD NDDNMLALSL ATLANSATAS PRLTLPPLSS PMNPNGHTSY 601 NGNMMNNNSG NGSNGSNSYS NGVTTAAATT TSAPHNLSIV SPNPTYSPNP 651 LSLYLTNSKN PLNSGLAPLS PSTSNIPFLK RNNVVTLNIS REASKSPISS 701 FVNDYRSPLG VSNPLMYSST INDYSNGTGI RQNSNNINPL DAGPSFSPLH 751 KKPKILNGND NSNLDSNNFD YSFTGNKQES NPSILNNNTN NNDNYRTSSM 801 NNNGNNYQAH SSKFGENEVI MSDHGKIYIC RECNRQFSSG HHLTRHKKSV 851 HSGEKPHSCP RCGKRFKRRD HVLQHLNKKI PCTQEMENTK LAES*

Amino Acid Sequence (or in FASTA format)

       1  MEIKPVEVID GVPVFKPSMM EFANFQYFID EITKFGIENG IVKVIPPKEW
      51  LELLEGSPPA ESLKTIQLDS PIQQQAKRWD KHENGVFSIE NEYDNKSYNL
     101  TQWKNLAESL DSRISQGDFN DKTLKENCRV DSQQDCYDLA QLQILESDFW
     151  KTIAFSKPFY AVDENSSIFP YDLTLWNLNN LPDSINSSNR RLLTGQSKCI
     201  FPWHLDEQNK CSINYLHFGA PKQWYSIPSA NTDQFLKILS KEPSSNKENC
     251  PAFIRHQNII TSPDFLRKNN IKFNRVVQFQ HEFIITFPYC MYSGFNYGYN
     301  FGESIEFILD QQAVVRKQPL KCGCGNKKEE RKSGPFSNLS YDSNESEQRG
     351  SITDNDNDLF QKVRSFDELL NHSSQELQNL EDNKNPLFSN INMNRPQSSS
     401  LRSTTPNGVN QFLNMNQTTI SRISSPLLSR MMDLSNIVEP TLDDPGSKFK
     451  RKVLTPQLPQ MNIPSNSSNF GTPSLTNTNS LLSNITATST NPSTTTNGSQ
     501  NHNNVNANGI NTSAAASINN NISSTNNSAN NSSSNNNVST VPSSMMHSST
     551  LNGTSGLGGD NDDNMLALSL ATLANSATAS PRLTLPPLSS PMNPNGHTSY
     601  NGNMMNNNSG NGSNGSNSYS NGVTTAAATT TSAPHNLSIV SPNPTYSPNP
     651  LSLYLTNSKN PLNSGLAPLS PSTSNIPFLK RNNVVTLNIS REASKSPISS
     701  FVNDYRSPLG VSNPLMYSST INDYSNGTGI RQNSNNINPL DAGPSFSPLH
     751  KKPKILNGND NSNLDSNNFD YSFTGNKQES NPSILNNNTN NNDNYRTSSM
     801  NNNGNNYQAH SSKFGENEVI MSDHGKIYIC RECNRQFSSG HHLTRHKKSV
     851  HSGEKPHSCP RCGKRFKRRD HVLQHLNKKI PCTQEMENTK LAES*

external links for Gis1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Gis1p

1)	Jang YK, et al. (1999) RPH1 and GIS1 are damage-responsive repressors of PHR1. Mol Cell Biol 19(11):7630-8
2)	Pedruzzi I, et al. (2000) Saccharomyces cerevisiae Ras/cAMP pathway controls post-diauxic shift element-dependent transcription through the zinc finger protein Gis1. EMBO J 19(11):2569-79
3)	Balciunas D and Ronne H (1999) Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells. Mol Gen Genet 262(4-5):589-99
4)	Oshiro J, et al. (2003) Regulation of the yeast DPP1-encoded diacylglycerol pyrophosphate phosphatase by transcription factor Gis1p. J Biol Chem 278(34):31495-503
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Tu S, et al. (2007) Identification of histone demethylases in Saccharomyces cerevisiae. J Biol Chem 282(19):14262-71
7)	Zhang N and Oliver SG (2010) The transcription activity of Gis1 is negatively modulated by proteasome-mediated limited proteolysis. J Biol Chem 285(9):6465-76
8)	Quan Z, et al. (2011) JmjN interacts with JmjC to ensure selective proteolysis of Gis1 by the proteasome. Microbiology 157(Pt 9):2694-701
9)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41