PDC2/YDR081C Protein Information

Standard Name	Pdc2p 1
Systematic Name	Ydr081cp
ORF Classification	Verified
Description	Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions (2, 3, 4, 5, 6, 7, 8)
Name Description	Pyruvate DeCarboxylase 1

Experimental Data
Molecules/cell	572 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	925
Molecular Weight (Da)	103,943
Isoelectric Point (pI)	6.4

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pdc2p (InterPro)
Physical Interactions	There are 7 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001682E6 \| P32896 MIPS: YDR081C NCBI: 1431551 \| 1907251 \| 308153471 \| 311117 \| 4112 \| 417455 \| 558252 \| 577808 \| 6320286 \| NP_010366.1 GenBank/EMBL/DDBJ: DAA11928.1 \| L19880 \| X65608 \| X82086 \| Z46796 \| Z74377 \| Z74378

Amino Acid Sequence (or in FASTA format)
1 MLSIQQRYNI CLMAERHPKW TQLELAKWAY ETFQLPKIPS QGTISRLLAR 51 KSTYMNCKEH EKDANRLRKP NNLLVRKILQ EWISQSLWNG IPITSPIIQD 101 TAQAVWHRIP AEHREGNGSF SYKWISNFLS KMDVNISVLD EELPKTPKVW 151 TFEERDVLKA YFSKIPPKDL FTLDEAFLSY NLPLDYAQYE ASSIQRRIEV 201 ATVMLCSNLD GSEKLKPVVV GKYDSYKSFR NYFPNEPNDP VSQSMLGTKM 251 AKKFDISYHS NRKAWLTSNL FHNWLVRWDK RLVAVNRKIW IVLDDSCCHR 301 IINLRLQNIK LVYTSSNSKF LPFNWGVWDE FKTRYRIQQY QALIDLQNRI 351 SKNIQNKNKS ERNECIPNGK KCLISFEQSQ LTMSNAFKFI KKAWDDIPVD 401 AIKANWKSSG LLPPEMIHLN ENVSMAFKKN EVLESVLNRL CDEYYCVKKW 451 EYEMLLDLNI ENKNTNFLST EELVESAIVE PCEPDFDTAP KGNEVHDDNF 501 DVSVFANEDD NNQNHLSMSQ ASHNPDYNSN HSNNAIENTN NRGSNNNNNN 551 NGSSNNINDN DSSVKYLQQN TVDNSTKTGN PGQPNISSME SQRNSSTTDL 601 VVDGNYDVNF NGLLNDPYNT MKQPGPLDYN VSTLIDKPNL FLSPDLDLST 651 VGVDMQLPSS EYFSEVFSSA IRNNEKAASD QNKSTDELPS STAMANSNSI 701 TTALLESRNQ AQPFDVPHMN GLLSDTSKSG HSVNSSNAIS QNSLNNFQHN 751 SASVAEASSP SITPSPVAIN STGAPARSII SAPIDSNSSA SSPSALEHLE 801 GAVSGMSPSS TTILSNLQTN INIAKSLSTI MKHAESNEIS LTKETINELN 851 FNYLTLLKRI KKTRKQLNSE SIKINSKNAQ DHLETLLSGA AAAAATSANN 901 LDLPTGGSNL PDSNNLHLPG NTGFF*

Amino Acid Sequence (or in FASTA format)

       1  MLSIQQRYNI CLMAERHPKW TQLELAKWAY ETFQLPKIPS QGTISRLLAR
      51  KSTYMNCKEH EKDANRLRKP NNLLVRKILQ EWISQSLWNG IPITSPIIQD
     101  TAQAVWHRIP AEHREGNGSF SYKWISNFLS KMDVNISVLD EELPKTPKVW
     151  TFEERDVLKA YFSKIPPKDL FTLDEAFLSY NLPLDYAQYE ASSIQRRIEV
     201  ATVMLCSNLD GSEKLKPVVV GKYDSYKSFR NYFPNEPNDP VSQSMLGTKM
     251  AKKFDISYHS NRKAWLTSNL FHNWLVRWDK RLVAVNRKIW IVLDDSCCHR
     301  IINLRLQNIK LVYTSSNSKF LPFNWGVWDE FKTRYRIQQY QALIDLQNRI
     351  SKNIQNKNKS ERNECIPNGK KCLISFEQSQ LTMSNAFKFI KKAWDDIPVD
     401  AIKANWKSSG LLPPEMIHLN ENVSMAFKKN EVLESVLNRL CDEYYCVKKW
     451  EYEMLLDLNI ENKNTNFLST EELVESAIVE PCEPDFDTAP KGNEVHDDNF
     501  DVSVFANEDD NNQNHLSMSQ ASHNPDYNSN HSNNAIENTN NRGSNNNNNN
     551  NGSSNNINDN DSSVKYLQQN TVDNSTKTGN PGQPNISSME SQRNSSTTDL
     601  VVDGNYDVNF NGLLNDPYNT MKQPGPLDYN VSTLIDKPNL FLSPDLDLST
     651  VGVDMQLPSS EYFSEVFSSA IRNNEKAASD QNKSTDELPS STAMANSNSI
     701  TTALLESRNQ AQPFDVPHMN GLLSDTSKSG HSVNSSNAIS QNSLNNFQHN
     751  SASVAEASSP SITPSPVAIN STGAPARSII SAPIDSNSSA SSPSALEHLE
     801  GAVSGMSPSS TTILSNLQTN INIAKSLSTI MKHAESNEIS LTKETINELN
     851  FNYLTLLKRI KKTRKQLNSE SIKINSKNAQ DHLETLLSGA AAAAATSANN
     901  LDLPTGGSNL PDSNNLHLPG NTGFF*

external links for Pdc2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Pdc2p

1)	Schmitt HD and Zimmermann FK (1982) Genetic analysis of the pyruvate decarboxylase reaction in yeast glycolysis. J Bacteriol 151(3):1146-52
2)	Velmurugan S, et al. (1997) Suppression of pdc2 regulating pyruvate decarboxylase synthesis in yeast. Genetics 145(3):587-94
3)	Hohmann S and Meacock PA (1998) Thiamin metabolism and thiamin diphosphate-dependent enzymes in the yeast Saccharomyces cerevisiae: genetic regulation. Biochim Biophys Acta 1385(2):201-19
4)	Hohmann S (1993) Characterisation of PDC2, a gene necessary for high level expression of pyruvate decarboxylase structural genes in Saccharomyces cerevisiae. Mol Gen Genet 241(5-6):657-66
5)	Mojzita D and Hohmann S (2006) Pdc2 coordinates expression of the THI regulon in the yeast Saccharomyces cerevisiae. Mol Genet Genomics 276(2):147-61
6)	Nosaka K, et al. (2008) Thiamin-dependent transactivation activity of PDC2 in Saccharomyces cerevisiae. FEBS Lett 582(29):3991-6
7)	Raghuram V, et al. (1994) PDC2, a yeast gene essential for synthesis of pyruvate decarboxylase, encodes a novel transcription factor J Genet 73():17-32
8)	Nosaka K, et al. (2012) Facilitated recruitment of Pdc2p, a yeast transcriptional activator, in response to thiamin starvation. FEMS Microbiol Lett 330(2):140-7
9)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41