CDC34/YDR054C Protein Information

Standard Name	Cdc34p 1
Systematic Name	Ydr054cp
Alias	Dna6p 2 , Ubc3p 3
ORF Classification	Verified
Description	Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress (4, 5)
Name Description	Cell Division Cycle

Experimental Data
Molecules/cell	8170 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	295
Molecular Weight (Da)	34,064
Isoelectric Point (pI)	3.97

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cdc34p (InterPro)
Physical Interactions	There are 87 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013795C \| P14682 MIPS: YDR054C NCBI: 136642 \| 1431497 \| 173124 \| 6320259 \| 706820 \| 798905 \| NP_010339.1 GenBank/EMBL/DDBJ: DAA11900.1 \| M21877 \| X84162 \| Z49209 \| Z74350
External Classifications	EC: 6.3.2.19 [Ubiquitin--protein ligase]

Amino Acid Sequence (or in FASTA format)
1 MSSRKSTASS LLLRQYRELT DPKKAIPSFH IELEDDSNIF TWNIGVMVLN 51 EDSIYHGGFF KAQMRFPEDF PFSPPQFRFT PAIYHPNVYR DGRLCISILH 101 QSGDPMTDEP DAETWSPVQT VESVLISIVS LLEDPNINSP ANVDAAVDYR 151 KNPEQYKQRV KMEVERSKQD IPKGFIMPTS ESAYISQSKL DEPESNKDMA 201 DNFWYDSDLD DDENGSVILQ DDDYDDGNNH IPFEDDDVYN YNDNDDDDER 251 IEFEDDDDDD DDSIDNDSVM DRKQPHKAED ESEDVEDVER VSKKI*

Amino Acid Sequence (or in FASTA format)

       1  MSSRKSTASS LLLRQYRELT DPKKAIPSFH IELEDDSNIF TWNIGVMVLN
      51  EDSIYHGGFF KAQMRFPEDF PFSPPQFRFT PAIYHPNVYR DGRLCISILH
     101  QSGDPMTDEP DAETWSPVQT VESVLISIVS LLEDPNINSP ANVDAAVDYR
     151  KNPEQYKQRV KMEVERSKQD IPKGFIMPTS ESAYISQSKL DEPESNKDMA
     201  DNFWYDSDLD DDENGSVILQ DDDYDDGNNH IPFEDDDVYN YNDNDDDDER
     251  IEFEDDDDDD DDSIDNDSVM DRKQPHKAED ESEDVEDVER VSKKI*

external links for Cdc34p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Cdc34p

1)	Goebl, M.G. (1989) Personal Communication, Mortimer Map Edition 10
2)	Dumas LB, et al. (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol Gen Genet 187(1):42-6
3)	Goebl MG, et al. (1988) The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme. Science 241(4871):1331-5
4)	Craig KL and Tyers M (1999) The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction. Prog Biophys Mol Biol 72(3):299-328
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41