NRG1/YDR043C Protein Information

Standard Name	Nrg1p 1
Systematic Name	Ydr043cp
ORF Classification	Verified
Description	Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	Negative Regulator of Glucose-repressed genes 1, 2

Experimental Data
Molecules/cell	556 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	231
Molecular Weight (Da)	26,743
Isoelectric Point (pI)	9.01

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Nrg1p (InterPro)
Physical Interactions	There are 10 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000130506 \| Q03125 MIPS: YDR043C NCBI: 11133517 \| 398365237 \| 4102584 \| 45269199 \| 854427 \| NP_010328.3 GenBank/EMBL/DDBJ: DAA11891.1 \| AF013167 \| AY557653 \| Z49812

Amino Acid Sequence (or in FASTA format)
1 MFYPYNYSNL NVSTMPALPG ISAFDGMQDE ENVEISPERK YQTLLPVLTN 51 SHVVENELKH KLNKTAFDFR YQTKSENGSE KWEPKYLITP NLQTRSVSFD 101 NSSVQYNSDS SEKSSLSQLT CNSSIIQQPE NGIVSNDAYN KMANSRYSLK 151 TRKQRTDPRN TLSDEEDLEQ RRKYICKICA RGFTTSGHLA RHNRIHTGEK 201 NHCCPYKGCT QRFSRHDNCL QHYRTHLKKG Q*

Amino Acid Sequence (or in FASTA format)

       1  MFYPYNYSNL NVSTMPALPG ISAFDGMQDE ENVEISPERK YQTLLPVLTN
      51  SHVVENELKH KLNKTAFDFR YQTKSENGSE KWEPKYLITP NLQTRSVSFD
     101  NSSVQYNSDS SEKSSLSQLT CNSSIIQQPE NGIVSNDAYN KMANSRYSLK
     151  TRKQRTDPRN TLSDEEDLEQ RRKYICKICA RGFTTSGHLA RHNRIHTGEK
     201  NHCCPYKGCT QRFSRHDNCL QHYRTHLKKG Q*

external links for Nrg1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Nrg1p

1)	Zhou H and Winston F (2001) NRG1 is required for glucose repression of the SUC2 and GAL genes of Saccharomyces cerevisiae. BMC Genet 2():5
2)	Park SH, et al. (1999) Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae. Mol Cell Biol 19(3):2044-50
3)	Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000
4)	Lamb TM and Mitchell AP (2003) The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae. Mol Cell Biol 23(2):677-86
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41