RAD61/YDR014W Protein Information Help

Standard Name Rad61p 1
Systematic Name Ydr014wp
Alias Wpl1p 2
ORF Classification Verified
Description Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; also negatively regulates chromosome condensation; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin (2, 3, 4, 5, 6)
Name Description RADiation sensitive 1
Experimental Data
Molecules/cell 721 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 647
Molecular Weight (Da) 74,721
Isoelectric Point (pI) 5.66

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rad61p (InterPro)
Physical Interactions There are 9 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000006B913 | Q99359
MIPS: YDR014W
NCBI: 1216221 | 1431436 | 6320217 | 642819 | 74664785 | NP_010297.1 | NM_001180322.1
GenBank/EMBL/DDBJ: DAA11860.1 | X95966 | Z48008 | Z74310
Amino Acid Sequence (or in FASTA format)
 
       1  MRAYGKRGPV LRTPFRSNKG LPSSSDVEFS DDDVNSVIPD VSSTISSSIA
      51  DHPIEGLLDE PRKAQDSSSS FDGANEKPSS QLDSKRNDQN VKIITSSDTS
     101  MAFMKDEKLS AFNFLDGSKA SKRKRRRTYQ KHDANITSSI EPDVQDEDSI
     151  TMHNEFESIR KIYNDINEFI LKLPRADDDI LNKMLENEMK MDDSIENNSI
     201  RTSKDKKYGK FRTILINKNK ENEIMGEEVD QKANTLSLNN ADNSNAEKEG
     251  LTSTNHYNEL KNMGDTIKYQ DDIEFLLSNS KSNDNTTVPI NEYFKKLLNL
     301  SLMIINDEEF FQYAKRYFKK EIIKLSFAQF RSDFPELILL QGYLLHKVSE
     351  SQSDFPPSFD NFSIELSKDD GKIRTKKNKH IKKLSHLNFE DFLRKTQFKT
     401  GLYYSLSLWE MHGNLSLDII KRISILASNK DLFSRHVKTF IPLLEKLITA
     451  SEFCHMYIEQ PEMFDSLISN LNNQFKDMLD DDSLIKILIL LTNMEVHNYT
     501  LWKEADMIFQ SSMNTILESI HPLTDAKVDN ILLHLGLCLN ICSRENSRLK
     551  LDGKLWYDMK TIFVKMIRDG SDTENRLVQG LFYLNFSFLI KQRKENSNLD
     601  PGELNLLLVE LEAFKSETSQ FNEGISNKIE IALNYLKSIY TSERITI*

external links for Rad61p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rad61p
1) Game JC, et al.  (2003) Use of a genome-wide approach to identify new genes that control resistance of Saccharomyces cerevisiae to ionizing radiation. Radiat Res 160(1):14-24
2) Rolef Ben-Shahar T, et al.  (2008) Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion. Science 321(5888):563-6
3) Kueng S, et al.  (2006) Wapl controls the dynamic association of cohesin with chromatin. Cell 127(5):955-67
4) Sutani T, et al.  (2009) Budding yeast Wpl1(Rad61)-Pds5 complex counteracts sister chromatid cohesion-establishing reaction. Curr Biol 19(6):492-7
5) Rowland BD, et al.  (2009) Building sister chromatid cohesion: smc3 acetylation counteracts an antiestablishment activity. Mol Cell 33(6):763-74
6) Lopez-Serra L, et al.  (2013) Budding yeast Wapl controls sister chromatid cohesion maintenance and chromosome condensation. Curr Biol 23(1):64-9
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41