HO/YDL227C Protein Information

Standard Name	Hop
Systematic Name	Ydl227cp
ORF Classification	Verified
Description	Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p (1, 2, 3, 4)
Name Description	HOmothallic switching endonuclease 2, 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	586
Molecular Weight (Da)	66,089
Isoelectric Point (pI)	9.82

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hop (InterPro)
Physical Interactions	There are 19 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012CB05 \| P09932 \| C9ELN8 MIPS: YDL227C NCBI: 2507047 \| 6319974 \| NP_010054.1 GenBank/EMBL/DDBJ: DAA11638.1 \| M14678 \| X90957 \| Z74275

Amino Acid Sequence (or in FASTA format)
1 MLSENTTILM ANGEIKDIAN VTANSYVMCA DGSAARVINV TQGYQKIYNI 51 QQKTKHRAFE GEPGRLDPRR RTVYQRLALQ CTAGHKLSVR VPTKPLLEKS 101 GRNATKYKVR WRNLQQCQTL DGRIIIIPKN HHKTFPMTVE GEFAAKRFIE 151 EMERSKGEYF NFDIEVRDLD YLDAQLRISS CIRFGPVLAG NGVLSKFLTG 201 RSDLVTPAVK SMAWMLGLWL GDSTTKEPEI SVDSLDPKLM ESLRENAKIW 251 GLYLTVCDDH VPLRAKHVRL HYGDGPDENR KTRNLRKNNP FWKAVTILKF 301 KRDLDGEKQI PEFMYGEHIE VREAFLAGLI DSDGYVVKKG EGPESYKIAI 351 QTVYSSIMDG IVHISRSLGM SATVTTRSAR EEIIEGRKVQ CQFTYDCNVA 401 GGTTSQNVLS YCRSGHKTRE VPPIIKREPV YFSFTDDFQG ESTVYGLTIE 451 GHKNFLLGNK IEVKSCRGCC VGEQLKISQK KNLKHCVACP RKGIKYFYKD 501 WSGKNRVCAR CYGRYKFSGH HCINCKYVPE AREVKKAKDK GEKLGITPEG 551 LPVKGPECIK CGGILQFDAV RGPHKSCGNN AGARIC*

Amino Acid Sequence (or in FASTA format)

       1  MLSENTTILM ANGEIKDIAN VTANSYVMCA DGSAARVINV TQGYQKIYNI
      51  QQKTKHRAFE GEPGRLDPRR RTVYQRLALQ CTAGHKLSVR VPTKPLLEKS
     101  GRNATKYKVR WRNLQQCQTL DGRIIIIPKN HHKTFPMTVE GEFAAKRFIE
     151  EMERSKGEYF NFDIEVRDLD YLDAQLRISS CIRFGPVLAG NGVLSKFLTG
     201  RSDLVTPAVK SMAWMLGLWL GDSTTKEPEI SVDSLDPKLM ESLRENAKIW
     251  GLYLTVCDDH VPLRAKHVRL HYGDGPDENR KTRNLRKNNP FWKAVTILKF
     301  KRDLDGEKQI PEFMYGEHIE VREAFLAGLI DSDGYVVKKG EGPESYKIAI
     351  QTVYSSIMDG IVHISRSLGM SATVTTRSAR EEIIEGRKVQ CQFTYDCNVA
     401  GGTTSQNVLS YCRSGHKTRE VPPIIKREPV YFSFTDDFQG ESTVYGLTIE
     451  GHKNFLLGNK IEVKSCRGCC VGEQLKISQK KNLKHCVACP RKGIKYFYKD
     501  WSGKNRVCAR CYGRYKFSGH HCINCKYVPE AREVKKAKDK GEKLGITPEG
     551  LPVKGPECIK CGGILQFDAV RGPHKSCGNN AGARIC*

external links for Hop

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Hop

1)	Nasmyth K (1993) Regulating the HO endonuclease in yeast. Curr Opin Genet Dev 3(2):286-94
2)	Jensen R, et al. (1983) Regulation of yeast mating-type interconversion: feedback control of HO gene expression by the mating-type locus. Proc Natl Acad Sci U S A 80(10):3035-9
3)	Strathern JN, et al. (1982) Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus. Cell 31(1):183-92
4)	Kostriken R, et al. (1983) A site-specific endonuclease essential for mating-type switching in Saccharomyces cerevisiae. Cell 35(1):167-74