PRR2/YDL214C Protein Information

Standard Name	Prr2p
Systematic Name	Ydl214cp
ORF Classification	Verified
Description	Serine/threonine protein kinase; inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance; PRR2 has a paralog, NPR1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	Pheromone Response Regulator 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	699
Molecular Weight (Da)	78,938
Isoelectric Point (pI)	6.69

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Prr2p (InterPro)
Physical Interactions	There are 42 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000069F0B \| Q12310 MIPS: YDL214C NCBI: 1429342 \| 1431358 \| 6319987 \| 74655042 \| NP_010067.1 GenBank/EMBL/DDBJ: DAA11650.1 \| X99000 \| Z74262
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MSLSRILRYN QRNNKTTASL TAEHAYSDNW AYSVSLGDPT SVGVNMAAKT 51 GEALNKSYDS VFSSLPVADS VPRTDFTASS RDDENTDVQK LTTSWMEKID 101 TKMPENISKI DSNIISSPMV SKVEARFIVP KGRLRKNSTD FTSSFSNSLS 151 LPKSYGKLIF FTSKKNSSST KKNLANDISD NKHNNNSSNT IGHNIPVTTA 201 TATCDEIACT STEHEYNVYE EERMFTTRVY SLEDSVSSLS TNPLDDTYSE 251 AVQVNTRHIE DTESTAHIRK HSYTTSLSSI KRLFKITSFS NNNSNSCDHQ 301 ESTVADDCAI SSSLKETTSS PVSTGSFSLM IENEDSDRDQ IIQALYSNIE 351 ASTDLVSRKY RDLDVVLGEG SGGKVKLVQR VLDNKVFALK EYRSKKKRES 401 ERKYIKNIIS EYCIASTLKN PNICETLEIL YEKGKIFQIL EYCEYDLFSL 451 VMSEKMHYEE ICCLFKQLIN GVKYLHDIGL SHRDLKLDNC VVTRRGILKL 501 IDFGASSVFH YPLSSQMIEA NGIVGSDPYL SPEVFYFNEY DPRALDVWSV 551 GIIFFCMITR RFPWKYPKVK DVQFKAFCSG RGVSSFKDLV TRPATDDSNN 601 YDNDGYEEGV IDMGPNFILH RLPEETHKIM RRILEVSPFR RITINGILQD 651 GWIKEIETCQ VVGAASPNEA SLRIINKGNH IHTNIDQRYA HIGGLHQRT*

Amino Acid Sequence (or in FASTA format)

       1  MSLSRILRYN QRNNKTTASL TAEHAYSDNW AYSVSLGDPT SVGVNMAAKT
      51  GEALNKSYDS VFSSLPVADS VPRTDFTASS RDDENTDVQK LTTSWMEKID
     101  TKMPENISKI DSNIISSPMV SKVEARFIVP KGRLRKNSTD FTSSFSNSLS
     151  LPKSYGKLIF FTSKKNSSST KKNLANDISD NKHNNNSSNT IGHNIPVTTA
     201  TATCDEIACT STEHEYNVYE EERMFTTRVY SLEDSVSSLS TNPLDDTYSE
     251  AVQVNTRHIE DTESTAHIRK HSYTTSLSSI KRLFKITSFS NNNSNSCDHQ
     301  ESTVADDCAI SSSLKETTSS PVSTGSFSLM IENEDSDRDQ IIQALYSNIE
     351  ASTDLVSRKY RDLDVVLGEG SGGKVKLVQR VLDNKVFALK EYRSKKKRES
     401  ERKYIKNIIS EYCIASTLKN PNICETLEIL YEKGKIFQIL EYCEYDLFSL
     451  VMSEKMHYEE ICCLFKQLIN GVKYLHDIGL SHRDLKLDNC VVTRRGILKL
     501  IDFGASSVFH YPLSSQMIEA NGIVGSDPYL SPEVFYFNEY DPRALDVWSV
     551  GIIFFCMITR RFPWKYPKVK DVQFKAFCSG RGVSSFKDLV TRPATDDSNN
     601  YDNDGYEEGV IDMGPNFILH RLPEETHKIM RRILEVSPFR RITINGILQD
     651  GWIKEIETCQ VVGAASPNEA SLRIINKGNH IHTNIDQRYA HIGGLHQRT*

external links for Prr2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Prr2p

1)	Burchett SA, et al. (2001) Identification of novel pheromone-response regulators through systematic overexpression of 120 protein kinases in yeast. J Biol Chem 276(28):26472-8
2)	Zhu H, et al. (2000) Analysis of yeast protein kinases using protein chips. Nat Genet 26(3):283-9
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Torres EM, et al. (2010) Identification of aneuploidy-tolerating mutations. Cell 143(1):71-83