UGA4/YDL210W Protein Information Help

Standard Name Uga4p 1, 2
Systematic Name Ydl210wp
ORF Classification Verified
Description Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane (1, 3, 4, 5)
Name Description Utilization of GAba 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 571
Molecular Weight (Da) 61,872
Isoelectric Point (pI) 6.61

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Uga4p (InterPro)
Transmembrane Domains There are 11 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 14 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000137AE9 | P32837
MIPS: YDL210W
NCBI: 1429346 | 1431350 | 418150 | 4750 | 6319991 | NP_010071.1
GenBank/EMBL/DDBJ: DAA11654.1 | X66472 | X99000 | Z74258
External Classifications TC: 2.A.3.4 [The Amino Acid/Choline Transporter (ACT) Family]
Amino Acid Sequence (or in FASTA format)
 
       1  MSMSSKNENK ISVEQRISTD IGQAYQLQGL GSNLRSIRSK TGAGEVNYID
      51  AAKSVNDNQL LAEIGYKQEL KRQFSTLQVF GIAFSIMGLL PSIASVMGGG
     101  LGGGPATLVW GWFVAAFFIL LVGITMAEHA SSIPTAGGLY YWTYYYAPEG
     151  YKEIISFIIG CSNSLALAAG VCSIDYGLAE EIAAAVTLTK DGNFEVTSGK
     201  LYGIFAGAVV VMCICTCVAS GAIARLQTLS IFANLFIIVL LFIALPIGTK
     251  HRMGGFNDGD FIFGKYENLS DWNNGWQFCL AGFMPAVWTI GSFDSCVHQS
     301  EEAKDAKKSV PIGIISSIAV CWILGWLIII CLMACINPDI DSVLDSKYGF
     351  ALAQIIYDSL GKKWAIAFMS LIAFCQFLMG ASITTAVSRQ VWAFSRDNGL
     401  PLSKYIKRVD SKYSVPFFAI LAACVGSLIL GLLCLIDDAA TDALFSLAVA
     451  GNNLAWSTPT VFRLTSGRDL FRPGPFYLGK IWSPIVAWTG VAFQLFIIIL
     501  VMFPSQQHGI TKSTMNYACV IGPGIWILAG IYYKVYKKKY YHGPATNLSD
     551  DDYTEAVGAD VIDTIMSKQE P*

external links for Uga4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome TCDB
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Uga4p
1) Andre B, et al.  (1993) Cloning and expression of the UGA4 gene coding for the inducible GABA-specific transport protein of Saccharomyces cerevisiae. Mol Gen Genet 237(1-2):17-25
2) Grenson M, et al.  (1987) 4-Aminobutyric acid (GABA) uptake in Baker's yeast Saccharomyces cerevisiae is mediated by the general amino acid permease, the proline permease and a GABA specific permease integrated into the GABA-catabolic pathway. Life Sci Adv Biochem 6:35-39
3) Correa Garcia S, et al.  (1997) Carbon and nitrogen sources regulate delta-aminolevulinic acid and gamma-aminobutyric acid transport in Saccharomyces cerevisiae. Int J Biochem Cell Biol 29(8-9):1097-101
4) Vissers S, et al.  (1989) Positive and negative regulatory elements control the expression of the UGA4 gene coding for the inducible 4-aminobutyric-acid-specific permease in Saccharomyces cerevisiae. Eur J Biochem 181(2):357-61
5) Uemura T, et al.  (2004) Uptake of GABA and putrescine by UGA4 on the vacuolar membrane in Saccharomyces cerevisiae. Biochem Biophys Res Commun 315(4):1082-7
6) Ramos F, et al.  (1985) Mutations affecting the enzymes involved in the utilization of 4-aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiae. Eur J Biochem 149(2):401-4