HEM3/YDL205C Protein Information

Standard Name	Hem3p 1
Systematic Name	Ydl205cp
Alias	Ole2p 2, 3
ORF Classification	Verified
Description	Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme (1, 2)
Name Description	HEMe biosynthesis 1, 2

Experimental Data
Molecules/cell	8480 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	327
Molecular Weight (Da)	36,674
Isoelectric Point (pI)	6.33

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hem3p (InterPro)
Physical Interactions	There are 3 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012C49D \| P28789 MIPS: YDL205C NCBI: 122846 \| 1429351 \| 1431340 \| 3771 \| 62467569 \| 6319996 \| NP_010076.1 GenBank/EMBL/DDBJ: DAA11659.1 \| AY899249 \| X99000 \| Z11745 \| Z74253
External Classifications	EC: 2.5.1.61 [Hydroxymethylbilane synthase]

Amino Acid Sequence (or in FASTA format)
1 MGPETLHIGG RKSKLAVIQS NHVLKLIEEK YPDYDCKVFT LQTLGDQIQF 51 KPLYSFGGKA LWTKELEDHL YHDDPSKKLD LIVHSLKDMP TLLPEGFELG 101 GITKRVDPTD CLVMPFYSAY KSLDDLPDGG IVGTSSVRRS AQLKRKYPHL 151 KFESVRGNIQ TRLQKLDDPK SPYQCIILAS AGLMRMGLEN RITQRFHSDT 201 MYHAVGQGAL GIEIRKGDTK MMKILDEICD LNATICCLSE RALMRTLEGG 251 CSVPIGVESK YNEETKKLLL KAIVVDVEGT EAVEDEIEML IENVKEDSMA 301 CGKILAERMI ADGAKKILDE INLDRIK*

Amino Acid Sequence (or in FASTA format)

       1  MGPETLHIGG RKSKLAVIQS NHVLKLIEEK YPDYDCKVFT LQTLGDQIQF
      51  KPLYSFGGKA LWTKELEDHL YHDDPSKKLD LIVHSLKDMP TLLPEGFELG
     101  GITKRVDPTD CLVMPFYSAY KSLDDLPDGG IVGTSSVRRS AQLKRKYPHL
     151  KFESVRGNIQ TRLQKLDDPK SPYQCIILAS AGLMRMGLEN RITQRFHSDT
     201  MYHAVGQGAL GIEIRKGDTK MMKILDEICD LNATICCLSE RALMRTLEGG
     251  CSVPIGVESK YNEETKKLLL KAIVVDVEGT EAVEDEIEML IENVKEDSMA
     301  CGKILAERMI ADGAKKILDE INLDRIK*

external links for Hem3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Hem3p

1)	Keng T, et al. (1992) Structure and regulation of yeast HEM3, the gene for porphobilinogen deaminase. Mol Gen Genet 234(2):233-43
2)	Gollub EG, et al. (1977) Yeast mutants deficient in heme biosynthesis and a heme mutant additionally blocked in cyclization of 2,3-oxidosqualene. J Biol Chem 252(9):2846-54
3)	Bard M (1972) Biochemical and genetic aspects of nystatin resistance in saccharomyces cerevisiae. J Bacteriol 111(3):649-57
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41