TRM8/YDL201W Protein Information

Standard Name	Trm8p 1
Systematic Name	Ydl201wp
ORF Classification	Verified
Description	Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p (1, 2, 3, 4)
Name Description	Transfer RNA Methyltransferase 1

Experimental Data
Molecules/cell	2630 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	286
Molecular Weight (Da)	33,391
Isoelectric Point (pI)	9.86

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Trm8p (InterPro)
Physical Interactions	There are 14 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006B1B4 \| Q12009 MIPS: YDL201W NCBI: 1429356 \| 1431332 \| 163310993 \| 163310994 \| 163310995 \| 163310996 \| 32171782 \| 6320000 \| NP_010080.1 GenBank/EMBL/DDBJ: DAA11663.1 \| X99000 \| Z74249
External Classifications	EC: 2.1.1.33 [tRNA (guanine-N(7)-)-methyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MKAKPLSQDP GSKRYAYRIN KEENRKELKH VKINESSLVQ EGQKIDLPKK 51 RYYRQRAHSN PFSDHQLEYP VSPQDMDWSK LYPYYKNAEN GQMTKKVTIA 101 DIGCGFGGLM IDLSPAFPED LILGMEIRVQ VTNYVEDRII ALRNNTASKH 151 GFQNINVLRG NAMKFLPNFF EKGQLSKMFF CFPDPHFKQR KHKARIITNT 201 LLSEYAYVLK EGGVVYTITD VKDLHEWMVK HLEEHPLFER LSKEWEENDE 251 CVKIMRNATE EGKKVERKKG DKFVACFTRL PTPAIL*

Amino Acid Sequence (or in FASTA format)

       1  MKAKPLSQDP GSKRYAYRIN KEENRKELKH VKINESSLVQ EGQKIDLPKK
      51  RYYRQRAHSN PFSDHQLEYP VSPQDMDWSK LYPYYKNAEN GQMTKKVTIA
     101  DIGCGFGGLM IDLSPAFPED LILGMEIRVQ VTNYVEDRII ALRNNTASKH
     151  GFQNINVLRG NAMKFLPNFF EKGQLSKMFF CFPDPHFKQR KHKARIITNT
     201  LLSEYAYVLK EGGVVYTITD VKDLHEWMVK HLEEHPLFER LSKEWEENDE
     251  CVKIMRNATE EGKKVERKKG DKFVACFTRL PTPAIL*

external links for Trm8p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Trm8p

1)	Alexandrov A, et al. (2002) Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA. RNA 8(10):1253-66
2)	Matsumoto K, et al. (2007) RNA recognition mechanism of eukaryote tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex). FEBS Lett 581(8):1599-604
3)	Matsumoto K, et al. (2008) Production of yeast tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex) in a wheat germ cell-free translation system. J Biotechnol 133(4):453-60
4)	Leulliot N, et al. (2008) Structure of the Yeast tRNA m7G Methylation Complex. Structure 16(1):52-61
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41