SNF3/YDL194W Protein Information

Standard Name	Snf3p 1, 2, 3
Systematic Name	Ydl194wp
ORF Classification	Verified
Description	Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication (4, 5, 6, 7, 8, 9)
Name Description	Sucrose NonFermenting 10

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	884
Molecular Weight (Da)	96,718
Isoelectric Point (pI)	5.13

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Snf3p (InterPro)
Transmembrane Domains	There are 12 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 7 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001682D2 \| P10870 MIPS: YDL194W NCBI: 1004301 \| 115502452 \| 1351078 \| 1431318 \| 172634 \| 6320007 \| NP_010087.1 GenBank/EMBL/DDBJ: DAA11669.1 \| J03246 \| X83276 \| Z74242
External Classifications	TC: 2.A.1.1 [The Sugar Porter (SP) Family]

Amino Acid Sequence (or in FASTA format)
1 MDPNSNSSSE TLRQEKQGFL DKALQRVKGI ALRRNNSNKD HTTDDTTGSI 51 RTPTSLQRQN SDRQSNMTSV FTDDISTIDD NSILFSEPPQ KQSMMMSICV 101 GVFVAVGGFL FGYDTGLINS ITSMNYVKSH VAPNHDSFTA QQMSILVSFL 151 SLGTFFGALT APFISDSYGR KPTIIFSTIF IFSIGNSLQV GAGGITLLIV 201 GRVISGIGIG AISAVVPLYQ AEATHKSLRG AIISTYQWAI TWGLLVSSAV 251 SQGTHARNDA SSYRIPIGLQ YVWSSFLAIG MFFLPESPRY YVLKDKLDEA 301 AKSLSFLRGV PVHDSGLLEE LVEIKATYDY EASFGSSNFI DCFISSKSRP 351 KQTLRMFTGI ALQAFQQFSG INFIFYYGVN FFNKTGVSNS YLVSFITYAV 401 NVVFNVPGLF FVEFFGRRKV LVVGGVIMTI ANFIVAIVGC SLKTVAAAKV 451 MIAFICLFIA AFSATWGGVV WVISAELYPL GVRSKCTAIC AAANWLVNFI 501 CALITPYIVD TGSHTSSLGA KIFFIWGSLN AMGVIVVYLT VYETKGLTLE 551 EIDELYIKSS TGVVSPKFNK DIRERALKFQ YDPLQRLEDG KNTFVAKRNN 601 FDDETPRNDF RNTISGEIDH SPNQKEVHSI PERVDIPTST EILESPNKSS 651 GMTVPVSPSL QDVPIPQTTE PAEIRTKYVD LGNGLGLNTY NRGPPSLSSD 701 SSEDYTEDEI GGPSSQGDQS NRSTMNDIND YMARLIHSTS TASNTTDKFS 751 GNQSTLRYHT ASSHSDTTEE DSNLMDLGNG LALNAYNRGP PSILMNSSDE 801 EANGGETSDN LNTAQDLAGM KERMAQFAQS YIDKRGGLEP ETQSNILSTS 851 LSVMADTNEH NNEILHSSEE NATNQPVNEN NDLK*

Amino Acid Sequence (or in FASTA format)

       1  MDPNSNSSSE TLRQEKQGFL DKALQRVKGI ALRRNNSNKD HTTDDTTGSI
      51  RTPTSLQRQN SDRQSNMTSV FTDDISTIDD NSILFSEPPQ KQSMMMSICV
     101  GVFVAVGGFL FGYDTGLINS ITSMNYVKSH VAPNHDSFTA QQMSILVSFL
     151  SLGTFFGALT APFISDSYGR KPTIIFSTIF IFSIGNSLQV GAGGITLLIV
     201  GRVISGIGIG AISAVVPLYQ AEATHKSLRG AIISTYQWAI TWGLLVSSAV
     251  SQGTHARNDA SSYRIPIGLQ YVWSSFLAIG MFFLPESPRY YVLKDKLDEA
     301  AKSLSFLRGV PVHDSGLLEE LVEIKATYDY EASFGSSNFI DCFISSKSRP
     351  KQTLRMFTGI ALQAFQQFSG INFIFYYGVN FFNKTGVSNS YLVSFITYAV
     401  NVVFNVPGLF FVEFFGRRKV LVVGGVIMTI ANFIVAIVGC SLKTVAAAKV
     451  MIAFICLFIA AFSATWGGVV WVISAELYPL GVRSKCTAIC AAANWLVNFI
     501  CALITPYIVD TGSHTSSLGA KIFFIWGSLN AMGVIVVYLT VYETKGLTLE
     551  EIDELYIKSS TGVVSPKFNK DIRERALKFQ YDPLQRLEDG KNTFVAKRNN
     601  FDDETPRNDF RNTISGEIDH SPNQKEVHSI PERVDIPTST EILESPNKSS
     651  GMTVPVSPSL QDVPIPQTTE PAEIRTKYVD LGNGLGLNTY NRGPPSLSSD
     701  SSEDYTEDEI GGPSSQGDQS NRSTMNDIND YMARLIHSTS TASNTTDKFS
     751  GNQSTLRYHT ASSHSDTTEE DSNLMDLGNG LALNAYNRGP PSILMNSSDE
     801  EANGGETSDN LNTAQDLAGM KERMAQFAQS YIDKRGGLEP ETQSNILSTS
     851  LSVMADTNEH NNEILHSSEE NATNQPVNEN NDLK*

external links for Snf3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	TCDB
YGOB	GeneMANIA	YeastRC Structure Prediction (Seattle)
YOGY

References cited on this page View Complete Literature Guide for Snf3p

1)	Neigeborn L and Carlson M (1984) Genes affecting the regulation of SUC2 gene expression by glucose repression in Saccharomyces cerevisiae. Genetics 108(4):845-58
2)	Neigeborn L, et al. (1986) Null mutations in the SNF3 gene of Saccharomyces cerevisiae cause a different phenotype than do previously isolated missense mutations. Mol Cell Biol 6(11):3569-74
3)	Celenza, J. and Carlson, M. (1989) Personal Communication, Mortimer Map Edition 10
4)	Vagnoli P, et al. (1998) The C-terminal domain of Snf3p mediates glucose-responsive signal transduction in Saccharomyces cerevisiae. FEMS Microbiol Lett 160(1):31-6
5)	Ozcan S, et al. (1996) Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression. Proc Natl Acad Sci U S A 93(22):12428-32
6)	Ko CH, et al. (1993) Roles of multiple glucose transporters in Saccharomyces cerevisiae. Mol Cell Biol 13(1):638-48
7)	Ozcan S, et al. (1998) Glucose sensing and signaling by two glucose receptors in the yeast Saccharomyces cerevisiae. EMBO J 17(9):2566-73
8)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
9)	Dietvorst J, et al. (2010) Amino acid residues involved in ligand preference of the Snf3 transporter-like sensor in Saccharomyces cerevisiae. Yeast 27(3):131-8
10)	Carlson M, et al. (1981) Mutants of yeast defective in sucrose utilization. Genetics 98(1):25-40