VMA1/YDL185W Protein Information

Standard Name	Vma1p 1
Systematic Name	Ydl185wp
Alias	Cls8p , Tfp1p 2
ORF Classification	Verified
Description	Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease (1, 3, 4)
Name Description	Vacuolar Membrane Atpase 1

Experimental Data
Molecules/cell	200000 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,071
Molecular Weight (Da)	118,636
Isoelectric Point (pI)	6.08

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Vma1p (InterPro)
Physical Interactions	There are 130 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013814F \| P17255 MIPS: YDL185W NCBI: 1004309 \| 137464 \| 1431301 \| 171650 \| 172907 \| 23200209 \| 23200210 \| 24158608 \| 24158904 \| 24158907 \| 253722799 \| 3114410 \| 3114411 \| 3417405 \| 3417406 \| 4205 \| 55670331 \| 55670332 \| 55670333 \| 55670334 \| 6320016 \| 9257100 \| 9257101 \| NP_010096.1 GenBank/EMBL/DDBJ: DAA11677.1 \| J05409 \| M21609 \| X58857 \| X83276 \| Z74233
External Classifications	EC: 3.1.-.- [Hydrolases acting on ester bonds ] EC: 3.6.3.14 [H(+)-transporting two-sector ATPase]

Amino Acid Sequence (or in FASTA format)
1 MAGAIENARK EIKRISLEDH AESEYGAIYS VSGPVVIAEN MIGCAMYELV 51 KVGHDNLVGE VIRIDGDKAT IQVYEETAGL TVGDPVLRTG KPLSVELGPG 101 LMETIYDGIQ RPLKAIKEES QSIYIPRGID TPALDRTIKW QFTPGKFQVG 151 DHISGGDIYG SVFENSLISS HKILLPPRSR GTITWIAPAG EYTLDEKILE 201 VEFDGKKSDF TLYHTWPVRV PRPVTEKLSA DYPLLTGQRV LDALFPCVQG 251 GTTCIPGAFG CGKTVISQSL SKYSNSDAII YVGCFAKGTN VLMADGSIEC 301 IENIEVGNKV MGKDGRPREV IKLPRGRETM YSVVQKSQHR AHKSDSSREV 351 PELLKFTCNA THELVVRTPR SVRRLSRTIK GVEYFEVITF EMGQKKAPDG 401 RIVELVKEVS KSYPISEGPE RANELVESYR KASNKAYFEW TIEARDLSLL 451 GSHVRKATYQ TYAPILYEND HFFDYMQKSK FHLTIEGPKV LAYLLGLWIG 501 DGLSDRATFS VDSRDTSLME RVTEYAEKLN LCAEYKDRKE PQVAKTVNLY 551 SKVVRGNGIR NNLNTENPLW DAIVGLGFLK DGVKNIPSFL STDNIGTRET 601 FLAGLIDSDG YVTDEHGIKA TIKTIHTSVR DGLVSLARSL GLVVSVNAEP 651 AKVDMNGTKH KISYAIYMSG GDVLLNVLSK CAGSKKFRPA PAAAFARECR 701 GFYFELQELK EDDYYGITLS DDSDHQFLLA NQVVVHNCGE RGNEMAEVLM 751 EFPELYTEMS GTKEPIMKRT TLVANTSNMP VAAREASIYT GITLAEYFRD 801 QGKNVSMIAD SSSRWAEALR EISGRLGEMP ADQGFPAYLG AKLASFYERA 851 GKAVALGSPD RTGSVSIVAA VSPAGGDFSD PVTTATLGIT QVFWGLDKKL 901 AQRKHFPSIN TSVSYSKYTN VLNKFYDSNY PEFPVLRDRM KEILSNAEEL 951 EQVVQLVGKS ALSDSDKITL DVATLIKEDF LQQNGYSTYD AFCPIWKTFD 1001 MMRAFISYHD EAQKAVANGA NWSKLADSTG DVKHAVSSSK FFEPSRGEKE 1051 VHGEFEKLLS TMQERFAEST D*

Amino Acid Sequence (or in FASTA format)

       1  MAGAIENARK EIKRISLEDH AESEYGAIYS VSGPVVIAEN MIGCAMYELV
      51  KVGHDNLVGE VIRIDGDKAT IQVYEETAGL TVGDPVLRTG KPLSVELGPG
     101  LMETIYDGIQ RPLKAIKEES QSIYIPRGID TPALDRTIKW QFTPGKFQVG
     151  DHISGGDIYG SVFENSLISS HKILLPPRSR GTITWIAPAG EYTLDEKILE
     201  VEFDGKKSDF TLYHTWPVRV PRPVTEKLSA DYPLLTGQRV LDALFPCVQG
     251  GTTCIPGAFG CGKTVISQSL SKYSNSDAII YVGCFAKGTN VLMADGSIEC
     301  IENIEVGNKV MGKDGRPREV IKLPRGRETM YSVVQKSQHR AHKSDSSREV
     351  PELLKFTCNA THELVVRTPR SVRRLSRTIK GVEYFEVITF EMGQKKAPDG
     401  RIVELVKEVS KSYPISEGPE RANELVESYR KASNKAYFEW TIEARDLSLL
     451  GSHVRKATYQ TYAPILYEND HFFDYMQKSK FHLTIEGPKV LAYLLGLWIG
     501  DGLSDRATFS VDSRDTSLME RVTEYAEKLN LCAEYKDRKE PQVAKTVNLY
     551  SKVVRGNGIR NNLNTENPLW DAIVGLGFLK DGVKNIPSFL STDNIGTRET
     601  FLAGLIDSDG YVTDEHGIKA TIKTIHTSVR DGLVSLARSL GLVVSVNAEP
     651  AKVDMNGTKH KISYAIYMSG GDVLLNVLSK CAGSKKFRPA PAAAFARECR
     701  GFYFELQELK EDDYYGITLS DDSDHQFLLA NQVVVHNCGE RGNEMAEVLM
     751  EFPELYTEMS GTKEPIMKRT TLVANTSNMP VAAREASIYT GITLAEYFRD
     801  QGKNVSMIAD SSSRWAEALR EISGRLGEMP ADQGFPAYLG AKLASFYERA
     851  GKAVALGSPD RTGSVSIVAA VSPAGGDFSD PVTTATLGIT QVFWGLDKKL
     901  AQRKHFPSIN TSVSYSKYTN VLNKFYDSNY PEFPVLRDRM KEILSNAEEL
     951  EQVVQLVGKS ALSDSDKITL DVATLIKEDF LQQNGYSTYD AFCPIWKTFD
    1001  MMRAFISYHD EAQKAVANGA NWSKLADSTG DVKHAVSSSK FFEPSRGEKE
    1051  VHGEFEKLLS TMQERFAEST D*

external links for Vma1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Vma1p

1)	Hirata R, et al. (1990) Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J Biol Chem 265(12):6726-33
2)	Ronne, H. (1993) Personal Communication, Mortimer Map Edition 12
3)	Cooper AA, et al. (1993) Protein splicing of the yeast TFP1 intervening protein sequence: a model for self-excision. EMBO J 12(6):2575-83
4)	Shih CK, et al. (1988) A dominant trifluoperazine resistance gene from Saccharomyces cerevisiae has homology with F0F1 ATP synthase and confers calcium-sensitive growth. Mol Cell Biol 8(8):3094-103
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41