LYS20/YDL182W Protein Information

Standard Name	Lys20p
Systematic Name	Ydl182wp
ORF Classification	Verified
Description	Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication (1, 2)
Name Description	LYSine requiring

Experimental Data
Molecules/cell	28100 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	428
Molecular Weight (Da)	47,098
Isoelectric Point (pI)	7.25

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Lys20p (InterPro)
Physical Interactions	There are 35 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012CAE3 \| P48570 MIPS: YDL182W NCBI: 1004312 \| 1061257 \| 1351812 \| 1431295 \| 1903292 \| 6320019 \| NP_010099.1 GenBank/EMBL/DDBJ: DAA11680.1 \| X83276 \| Z67750 \| Z74229 \| Z74230
External Classifications	EC: 2.3.3.14 [Homocitrate synthase]

Amino Acid Sequence (or in FASTA format)
1 MTAAKPNPYA AKPGDYLSNV NNFQLIDSTL REGEQFANAF FDTEKKIEIA 51 RALDDFGVDY IELTSPVASE QSRKDCEAIC KLGLKAKILT HIRCHMDDAK 101 VAVETGVDGV DVVIGTSKFL RQYSHGKDMN YIAKSAVEVI EFVKSKGIEI 151 RFSSEDSFRS DLVDLLNIYK TVDKIGVNRV GIADTVGCAN PRQVYELIRT 201 LKSVVSCDIE CHFHNDTGCA IANAYTALEG GARLIDVSVL GIGERNGITP 251 LGGLMARMIV AAPDYVKSKY KLHKIRDIEN LVADAVEVNI PFNNPITGFC 301 AFTHKAGIHA KAILANPSTY EILDPHDFGM KRYIHFANRL TGWNAIKARV 351 DQLNLNLTDD QIKEVTAKIK KLGDVRSLNI DDVDSIIKNF HAEVSTPQVL 401 SAKKNKKNDS DVPELATIPA AKRTKPSA*

Amino Acid Sequence (or in FASTA format)

       1  MTAAKPNPYA AKPGDYLSNV NNFQLIDSTL REGEQFANAF FDTEKKIEIA
      51  RALDDFGVDY IELTSPVASE QSRKDCEAIC KLGLKAKILT HIRCHMDDAK
     101  VAVETGVDGV DVVIGTSKFL RQYSHGKDMN YIAKSAVEVI EFVKSKGIEI
     151  RFSSEDSFRS DLVDLLNIYK TVDKIGVNRV GIADTVGCAN PRQVYELIRT
     201  LKSVVSCDIE CHFHNDTGCA IANAYTALEG GARLIDVSVL GIGERNGITP
     251  LGGLMARMIV AAPDYVKSKY KLHKIRDIEN LVADAVEVNI PFNNPITGFC
     301  AFTHKAGIHA KAILANPSTY EILDPHDFGM KRYIHFANRL TGWNAIKARV
     351  DQLNLNLTDD QIKEVTAKIK KLGDVRSLNI DDVDSIIKNF HAEVSTPQVL
     401  SAKKNKKNDS DVPELATIPA AKRTKPSA*

external links for Lys20p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Lys20p

1)	Ramos F, et al. (1996) Identification of a gene encoding a homocitrate synthase isoenzyme of Saccharomyces cerevisiae. Yeast 12(13):1315-20
2)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41