DLD1/YDL174C Protein Information

Standard Name	Dld1p
Systematic Name	Ydl174cp
ORF Classification	Verified
Description	D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane (1, 2, 3, 4)
Name Description	D-Lactate Dehydrogenase 3

Experimental Data
Molecules/cell	10800 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	587
Molecular Weight (Da)	65,292
Isoelectric Point (pI)	6.81

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Dld1p (InterPro)
Physical Interactions	There are 4 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000129458 \| P32891 MIPS: YDL174C NCBI: 1061264 \| 1431279 \| 2506961 \| 297922 \| 6320027 \| NP_010107.1 GenBank/EMBL/DDBJ: DAA11688.1 \| X66052 \| Z67750 \| Z74222
External Classifications	EC: 1.1.2.4 [D-lactate dehydrogenase (cytochrome)]

Amino Acid Sequence (or in FASTA format)
1 MLWKRTCTRL IKPIAQPRGR LVRRSCYRYA STGTGSTDSS SQWLKYSVIA 51 SSATLFGYLF AKNLYSRETK EDLIEKLEMV KKIDPVNSTL KLSSLDSPDY 101 LHDPVKIDKV VEDLKQVLGN KPENYSDAKS DLDAHSDTYF NTHHPSPEQR 151 PRIILFPHTT EEVSKILKIC HDNNMPVVPF SGGTSLEGHF LPTRIGDTIT 201 VDLSKFMNNV VKFDKLDLDI TVQAGLPWED LNDYLSDHGL MFGCDPGPGA 251 QIGGCIANSC SGTNAYRYGT MKENIINMTI VLPDGTIVKT KKRPRKSSAG 301 YNLNGLFVGS EGTLGIVTEA TVKCHVKPKA ETVAVVSFDT IKDAAACASN 351 LTQSGIHLNA MELLDENMMK LINASESTDR CDWVEKPTMF FKIGGRSPNI 401 VNALVDEVKA VAQLNHCNSF QFAKDDDEKL ELWEARKVAL WSVLDADKSK 451 DKSAKIWTTD VAVPVSQFDK VIHETKKDMQ ASKLINAIVG HAGDGNFHAF 501 IVYRTPEEHE TCSQLVDRMV KRALNAEGTC TGEHGVGIGK REYLLEELGE 551 APVDLMRKIK LAIDPKRIMN PDKIFKTDPN EPANDYR*

Amino Acid Sequence (or in FASTA format)

       1  MLWKRTCTRL IKPIAQPRGR LVRRSCYRYA STGTGSTDSS SQWLKYSVIA
      51  SSATLFGYLF AKNLYSRETK EDLIEKLEMV KKIDPVNSTL KLSSLDSPDY
     101  LHDPVKIDKV VEDLKQVLGN KPENYSDAKS DLDAHSDTYF NTHHPSPEQR
     151  PRIILFPHTT EEVSKILKIC HDNNMPVVPF SGGTSLEGHF LPTRIGDTIT
     201  VDLSKFMNNV VKFDKLDLDI TVQAGLPWED LNDYLSDHGL MFGCDPGPGA
     251  QIGGCIANSC SGTNAYRYGT MKENIINMTI VLPDGTIVKT KKRPRKSSAG
     301  YNLNGLFVGS EGTLGIVTEA TVKCHVKPKA ETVAVVSFDT IKDAAACASN
     351  LTQSGIHLNA MELLDENMMK LINASESTDR CDWVEKPTMF FKIGGRSPNI
     401  VNALVDEVKA VAQLNHCNSF QFAKDDDEKL ELWEARKVAL WSVLDADKSK
     451  DKSAKIWTTD VAVPVSQFDK VIHETKKDMQ ASKLINAIVG HAGDGNFHAF
     501  IVYRTPEEHE TCSQLVDRMV KRALNAEGTC TGEHGVGIGK REYLLEELGE
     551  APVDLMRKIK LAIDPKRIMN PDKIFKTDPN EPANDYR*

external links for Dld1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Dld1p

1)	Lodi T, et al. (1999) Regulation of the Saccharomyces cerevisiae DLD1 gene encoding the mitochondrial protein D-lactate ferricytochrome c oxidoreductase by HAP1 and HAP2/3/4/5. Mol Gen Genet 262(4-5):623-32
2)	Chelstowska A, et al. (1999) Signalling between mitochondria and the nucleus regulates the expression of a new D-lactate dehydrogenase activity in yeast. Yeast 15(13):1377-91
3)	Rojo EE, et al. (1998) Sorting of D-lactate dehydrogenase to the inner membrane of mitochondria. Analysis of topogenic signal and energetic requirements. J Biol Chem 273(14):8040-7
4)	Lodi T and Ferrero I (1993) Isolation of the DLD gene of Saccharomyces cerevisiae encoding the mitochondrial enzyme D-lactate ferricytochrome c oxidoreductase. Mol Gen Genet 238(3):315-24
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41