SFA1/YDL168W Protein Information Help

Standard Name Sfa1p 1
Systematic Name Ydl168wp
Alias Adh5p 2
ORF Classification Verified
Description Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress (2, 3, 4, 5)
Name Description Sensitive to FormAldehyde 3
Experimental Data
Molecules/cell 8370 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 386
Molecular Weight (Da) 41,042
Isoelectric Point (pI) 6.74

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Sfa1p (InterPro)
Physical Interactions There are 13 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012A4DD | P32771
MIPS: YDL168W
NCBI: 1061271 | 1431268 | 288591 | 417769 | 6320033 | NP_010113.1 | NM_001180228.1
GenBank/EMBL/DDBJ: CAA48161.1 | CAA91578.1 | CAA98742.1 | DAA11693.1 | X68020 | Z67750 | Z74216
External Classifications EC: 1.1.1.- [Oxidoreductases With NAD+ or NADP+ as acceptor]
EC: 1.1.1.1 [Alcohol dehydrogenase]
EC: 1.1.1.284 [S-(hydroxymethyl)glutathione dehydrogenase]
Amino Acid Sequence (or in FASTA format)
       1  MSAATVGKPI KCIAAVAYDA KKPLSVEEIT VDAPKAHEVR IKIEYTAVCH
      51  TDAYTLSGSD PEGLFPCVLG HEGAGIVESV GDDVITVKPG DHVIALYTAE
     101  CGKCKFCTSG KTNLCGAVRA TQGKGVMPDG TTRFHNAKGE DIYHFMGCST
     151  FSEYTVVADV SVVAIDPKAP LDAACLLGCG VTTGFGAALK TANVQKGDTV
     201  AVFGCGTVGL SVIQGAKLRG ASKIIAIDIN NKKKQYCSQF GATDFVNPKE
     251  DLAKDQTIVE KLIEMTDGGL DFTFDCTGNT KIMRDALEAC HKGWGQSIII
     301  GVAAAGEEIS TRPFQLVTGR VWKGSAFGGI KGRSEMGGLI KDYQKGALKV
     351  EEFITHRRPF KEINQAFEDL HNGDCLRTVL KSDEIK*              

external links for Sfa1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) CYC2008 (complexes) MIPS YeastGFP
Candida (CGD) Complexome Pfam domains YeastRC Public Image Repository
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Sfa1p
1) Mack, M. and Brendel, M.  (1989) Personal Communication, Mortimer Map Edition 10
2) Grey M, et al.  (1996) Overexpression of ADH1 confers hyper-resistance to formaldehyde in Saccharomyces cerevisiae. Curr Genet 29(5):437-40
3) Wehner EP, et al.  (1993) Molecular structure and genetic regulation of SFA, a gene responsible for resistance to formaldehyde in Saccharomyces cerevisiae, and characterization of its protein product. Mol Gen Genet 237(3):351-8
4) Rep M, et al.  (2001) The Saccharomyces cerevisiae Sko1p transcription factor mediates HOG pathway-dependent osmotic regulation of a set of genes encoding enzymes implicated in protection from oxidative damage. Mol Microbiol 40(5):1067-83
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41