SFA1/YDL168W Protein Information

Standard Name	Sfa1p 1
Systematic Name	Ydl168wp
Alias	Adh5p 2
ORF Classification	Verified
Description	Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress (2, 3, 4, 5)
Name Description	Sensitive to FormAldehyde 3

Experimental Data
Molecules/cell	8370 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	386
Molecular Weight (Da)	41,042
Isoelectric Point (pI)	6.74

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sfa1p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012A4DD \| P32771 MIPS: YDL168W NCBI: 1061271 \| 1431268 \| 288591 \| 417769 \| 6320033 \| NP_010113.1 GenBank/EMBL/DDBJ: DAA11693.1 \| X68020 \| Z67750 \| Z74216
External Classifications	EC: 1.1.1.1 [Alcohol dehydrogenase] EC: 1.1.1.284 [S-(hydroxymethyl)glutathione dehydrogenase]

Amino Acid Sequence (or in FASTA format)
1 MSAATVGKPI KCIAAVAYDA KKPLSVEEIT VDAPKAHEVR IKIEYTAVCH 51 TDAYTLSGSD PEGLFPCVLG HEGAGIVESV GDDVITVKPG DHVIALYTAE 101 CGKCKFCTSG KTNLCGAVRA TQGKGVMPDG TTRFHNAKGE DIYHFMGCST 151 FSEYTVVADV SVVAIDPKAP LDAACLLGCG VTTGFGAALK TANVQKGDTV 201 AVFGCGTVGL SVIQGAKLRG ASKIIAIDIN NKKKQYCSQF GATDFVNPKE 251 DLAKDQTIVE KLIEMTDGGL DFTFDCTGNT KIMRDALEAC HKGWGQSIII 301 GVAAAGEEIS TRPFQLVTGR VWKGSAFGGI KGRSEMGGLI KDYQKGALKV 351 EEFITHRRPF KEINQAFEDL HNGDCLRTVL KSDEIK*

Amino Acid Sequence (or in FASTA format)

       1  MSAATVGKPI KCIAAVAYDA KKPLSVEEIT VDAPKAHEVR IKIEYTAVCH
      51  TDAYTLSGSD PEGLFPCVLG HEGAGIVESV GDDVITVKPG DHVIALYTAE
     101  CGKCKFCTSG KTNLCGAVRA TQGKGVMPDG TTRFHNAKGE DIYHFMGCST
     151  FSEYTVVADV SVVAIDPKAP LDAACLLGCG VTTGFGAALK TANVQKGDTV
     201  AVFGCGTVGL SVIQGAKLRG ASKIIAIDIN NKKKQYCSQF GATDFVNPKE
     251  DLAKDQTIVE KLIEMTDGGL DFTFDCTGNT KIMRDALEAC HKGWGQSIII
     301  GVAAAGEEIS TRPFQLVTGR VWKGSAFGGI KGRSEMGGLI KDYQKGALKV
     351  EEFITHRRPF KEINQAFEDL HNGDCLRTVL KSDEIK*

external links for Sfa1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Sfa1p

1)	Mack, M. and Brendel, M. (1989) Personal Communication, Mortimer Map Edition 10
2)	Grey M, et al. (1996) Overexpression of ADH1 confers hyper-resistance to formaldehyde in Saccharomyces cerevisiae. Curr Genet 29(5):437-40
3)	Wehner EP, et al. (1993) Molecular structure and genetic regulation of SFA, a gene responsible for resistance to formaldehyde in Saccharomyces cerevisiae, and characterization of its protein product. Mol Gen Genet 237(3):351-8
4)	Rep M, et al. (2001) The Saccharomyces cerevisiae Sko1p transcription factor mediates HOG pathway-dependent osmotic regulation of a set of genes encoding enzymes implicated in protection from oxidative damage. Mol Microbiol 40(5):1067-83
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41