STE7/YDL159W Protein Information

Standard Name	Ste7p
Systematic Name	Ydl159wp
ORF Classification	Verified
Description	Signal transducing MAP kinase kinase involved in pheromone response, where it phosphorylates Fus3p, and in the pseudohyphal/invasive growth pathway, through phosphorylation of Kss1p; phosphorylated by Ste11p, degraded by ubiquitin pathway (1, 2)
Name Description	STErile

Experimental Data
Molecules/cell	672 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	515
Molecular Weight (Da)	57,708
Isoelectric Point (pI)	9.92

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ste7p (InterPro)
Physical Interactions	There are 88 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001360E0 \| P06784 MIPS: YDL159W NCBI: 1061280 \| 1321946 \| 134968 \| 1431251 \| 172762 \| 6320042 \| NP_010122.1 GenBank/EMBL/DDBJ: DAA11702.1 \| M14097 \| X97751 \| Z67750 \| Z74207
External Classifications	EC: 2.7.12.2 [Mitogen-activated protein kinase kinase]

Amino Acid Sequence (or in FASTA format)
1 MFQRKTLQRR NLKGLNLNLH PDVGNNGQLQ EKTETHQGQS RIEGHVMSNI 51 NAIQNNSNLF LRRGIKKKLT LDAFGDDQAI SKPNTVVIQQ PQNEPVLVLS 101 SLSQSPCVSS SSSLSTPCII DAYSNNFGLS PSSTNSTPST IQGLSNIATP 151 VENEHSISLP PLEESLSPAA ADLKDTLSGT SNGNYIQLQD LVQLGKIGAG 201 NSGTVVKALH VPDSKIVAKK TIPVEQNNST IINQLVRELS IVKNVKPHEN 251 IITFYGAYYN QHINNEIIIL MEYSDCGSLD KILSVYKRFV QRGTVSSKKT 301 WFNELTISKI AYGVLNGLDH LYRQYKIIHR DIKPSNVLIN SKGQIKLCDF 351 GVSKKLINSI ADTFVGTSTY MSPERIQGNV YSIKGDVWSL GLMIIELVTG 401 EFPLGGHNDT PDGILDLLQR IVNEPSPRLP KDRIYSKEMT DFVNRCCIKN 451 ERERSSIHEL LHHDLIMKYV SPSKDDKFRH WCRKIKSKIK EDKRIKREAL 501 DRAKLEKKQS ERSTH*

Amino Acid Sequence (or in FASTA format)

       1  MFQRKTLQRR NLKGLNLNLH PDVGNNGQLQ EKTETHQGQS RIEGHVMSNI
      51  NAIQNNSNLF LRRGIKKKLT LDAFGDDQAI SKPNTVVIQQ PQNEPVLVLS
     101  SLSQSPCVSS SSSLSTPCII DAYSNNFGLS PSSTNSTPST IQGLSNIATP
     151  VENEHSISLP PLEESLSPAA ADLKDTLSGT SNGNYIQLQD LVQLGKIGAG
     201  NSGTVVKALH VPDSKIVAKK TIPVEQNNST IINQLVRELS IVKNVKPHEN
     251  IITFYGAYYN QHINNEIIIL MEYSDCGSLD KILSVYKRFV QRGTVSSKKT
     301  WFNELTISKI AYGVLNGLDH LYRQYKIIHR DIKPSNVLIN SKGQIKLCDF
     351  GVSKKLINSI ADTFVGTSTY MSPERIQGNV YSIKGDVWSL GLMIIELVTG
     401  EFPLGGHNDT PDGILDLLQR IVNEPSPRLP KDRIYSKEMT DFVNRCCIKN
     451  ERERSSIHEL LHHDLIMKYV SPSKDDKFRH WCRKIKSKIK EDKRIKREAL
     501  DRAKLEKKQS ERSTH*

external links for Ste7p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
YGOB	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ste7p

1)	O'Rourke SM and Herskowitz I (1998) The Hog1 MAPK prevents cross talk between the HOG and pheromone response MAPK pathways in Saccharomyces cerevisiae. Genes Dev 12(18):2874-86
2)	Wang Y, et al. (2003) Regulation of Ste7 ubiquitination by Ste11 phosphorylation and the Skp1-Cullin-F-box complex. J Biol Chem 278(25):22284-9
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41