SCM3/YDL139C Protein Information

Standard Name	Scm3p 1
Systematic Name	Ydl139cp
ORF Classification	Verified
Description	Nonhistone component of centromeric chromatin; binds to histone H3 variant, Cse4p, and recruits it to centromeres; involved in the assembly and maintenance of Cse4-H4 at centromeres; required for kinetochore assembly and G2/M progression; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP (1, 2, 3, 4, 5, 6, 7)
Name Description	Suppressor of Chromosome Missegregation 2

Experimental Data
Molecules/cell	556 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	223
Molecular Weight (Da)	25,754
Isoelectric Point (pI)	6.65

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Scm3p (InterPro)
Physical Interactions	There are 36 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001D476E \| Q12334 MIPS: YDL139C NCBI: 115502830 \| 1419222 \| 1431215 \| 326327711 \| 37362629 \| 45270218 \| NP_010142.2 GenBank/EMBL/DDBJ: DAA11719.1 \| AY558164 \| X96876 \| Z74187

Amino Acid Sequence (or in FASTA format)
1 MKTNKKISKR RSLKNLHGAL KGLLKESGKK SESKIRKHSD CNPVHRVYPP 51 NIEKRKTKKD DGISRPIAER NGHVYIMSKE NHIIPKLTDD EVMERHKLAD 101 ENMRKVWSNI ISKYESIEEQ GDLVDLKTGE IVEDNGHIKT LTANNSTKDK 151 RTKYTSVLRD IIDISDEEDG DKNDEYTLWA NDSEASDSEV DADNDTEEEK 201 DEKLIDADFK KYEAKLSKRI LRD*

Amino Acid Sequence (or in FASTA format)

       1  MKTNKKISKR RSLKNLHGAL KGLLKESGKK SESKIRKHSD CNPVHRVYPP
      51  NIEKRKTKKD DGISRPIAER NGHVYIMSKE NHIIPKLTDD EVMERHKLAD
     101  ENMRKVWSNI ISKYESIEEQ GDLVDLKTGE IVEDNGHIKT LTANNSTKDK
     151  RTKYTSVLRD IIDISDEEDG DKNDEYTLWA NDSEASDSEV DADNDTEEEK
     201  DEKLIDADFK KYEAKLSKRI LRD*

external links for Scm3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Scm3p

1)	Chen Y, et al. (2000) The N terminus of the centromere H3-like protein Cse4p performs an essential function distinct from that of the histone fold domain. Mol Cell Biol 20(18):7037-48
2)	Stoler S, et al. (2007) Scm3, an essential Saccharomyces cerevisiae centromere protein required for G2/M progression and Cse4 localization. Proc Natl Acad Sci U S A 104(25):10571-6
3)	Camahort R, et al. (2007) Scm3 is essential to recruit the histone h3 variant cse4 to centromeres and to maintain a functional kinetochore. Mol Cell 26(6):853-65
4)	Mizuguchi G, et al. (2007) Nonhistone Scm3 and histones CenH3-H4 assemble the core of centromere-specific nucleosomes. Cell 129(6):1153-64
5)	Aravind L, et al. (2007) Domain architectures of the Scm3p protein provide insights into centromere function and evolution. Cell Cycle 6(20):2511-5
6)	Hewawasam G, et al. (2010) Psh1 Is an E3 Ubiquitin Ligase that Targets the Centromeric Histone Variant Cse4. Mol Cell 40(3):444-54
7)	Mishra PK, et al. (2011) Misregulation of Scm3p/HJURP Causes Chromosome Instability in Saccharomyces cerevisiae and Human Cells. PLoS Genet 7(9):e1002303
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41