PPH21/YDL134C Protein Information

Standard Name	Pph21p
Systematic Name	Ydl134cp
Alias	Pph1p
ORF Classification	Verified
Description	Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description	Protein PHosphatase

Experimental Data
Molecules/cell	5620 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	369
Molecular Weight (Da)	41,938
Isoelectric Point (pI)	4.46

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pph21p (InterPro)
Physical Interactions	There are 79 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000531B7 \| P23594 MIPS: YDL134C NCBI: 129329 \| 1419227 \| 1431205 \| 4201 \| 4203 \| 6320067 \| NP_010147.1 GenBank/EMBL/DDBJ: DAA11724.1 \| X56261 \| X58856 \| X96876 \| Z74182
External Classifications	EC: 3.1.3.16 [Phosphoprotein phosphatase]

Amino Acid Sequence (or in FASTA format)
1 MDTDLDVPMQ DAVTEQLTPT VSEDMDLNNN SSDNNAEEFS VDDLKPGSSG 51 IADHKSSKPL ELNNTNINQL DQWIEHLSKC EPLSEDDVAR LCKMAVDVLQ 101 FEENVKPINV PVTICGDVHG QFHDLLELFK IGGPCPDTNY LFMGDYVDRG 151 YYSVETVSYL VAMKVRYPHR ITILRGNHES RQITQVYGFY DECLRKYGSA 201 NVWKMFTDLF DYFPITALVD NKIFCLHGGL SPMIETIDQV RELNRIQEVP 251 HEGPMCDLLW SDPDDRGGWG ISPRGAGFTF GQDVSEQFNH TNDLSLIARA 301 HQLVMEGYAW SHQQNVVTIF SAPNYCYRCG NQAAIMEVDE NHNRQFLQYD 351 PSVRPGEPSV SRKTPDYFL*

Amino Acid Sequence (or in FASTA format)

       1  MDTDLDVPMQ DAVTEQLTPT VSEDMDLNNN SSDNNAEEFS VDDLKPGSSG
      51  IADHKSSKPL ELNNTNINQL DQWIEHLSKC EPLSEDDVAR LCKMAVDVLQ
     101  FEENVKPINV PVTICGDVHG QFHDLLELFK IGGPCPDTNY LFMGDYVDRG
     151  YYSVETVSYL VAMKVRYPHR ITILRGNHES RQITQVYGFY DECLRKYGSA
     201  NVWKMFTDLF DYFPITALVD NKIFCLHGGL SPMIETIDQV RELNRIQEVP
     251  HEGPMCDLLW SDPDDRGGWG ISPRGAGFTF GQDVSEQFNH TNDLSLIARA
     301  HQLVMEGYAW SHQQNVVTIF SAPNYCYRCG NQAAIMEVDE NHNRQFLQYD
     351  PSVRPGEPSV SRKTPDYFL*

external links for Pph21p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Pph21p

1)	Lin FC and Arndt KT (1995) The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis. EMBO J 14(12):2745-59
2)	Sneddon AA, et al. (1990) Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes. EMBO J 9(13):4339-46
3)	Wei H, et al. (2001) Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p. J Biol Chem 276(2):1570-7
4)	Zabrocki P, et al. (2002) Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol Microbiol 43(4):835-42
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41