QRI7/YDL104C Protein Information Help

Standard Name Qri7p 1
Systematic Name Ydl104cp
ORF Classification Verified
Description Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex (2, 3, 4, 5, 6, 7)
Experimental Data
Molecules/cell 1400 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 407
Molecular Weight (Da) 45,543
Isoelectric Point (pI) 7.67

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Qri7p (InterPro)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000132E6F | P43122
MIPS: YDL104C
NCBI: 1172805 | 1199545 | 1431146 | 491668761 | 6320099 | 683704 | NP_010179.1 | NM_001180163.1
GenBank/EMBL/DDBJ: CAA55926.1 | CAA64909.1 | CAA98671.1 | DAA11756.1 | X79380 | X95644 | Z74152
External Classifications EC: 2.3.1.- [Acyltransferases transferring groups other than amino-acyl groups]
EC: 3.4.24.- [Metalloendopeptidases]
Amino Acid Sequence (or in FASTA format)
       1  MISIKGTGRF LLDNYRIWQR RAFNRPIQLR KGYKVLAIET SCDDTCVSVL
      51  DRFSKSAAPN VLANLKDTLD SIDEGGIIPT KAHIHHQARI GPLTERALIE
     101  SNAREGIDLI CVTRGPGMPG SLSGGLDFAK GLAVAWNKPL IGVHHMLGHL
     151  LIPRMGTNGK VPQFPFVSLL VSGGHTTFVL SRAIDDHEIL CDTIDIAVGD
     201  SLDKCGRELG FKGTMIAREM EKFINQDIND QDFALKLEMP SPLKNSASKR
     251  NMLSFSFSAF ITALRTNLTK LGKTEIQELP EREIRSIAYQ VQESVFDHII
     301  NKLKHVLKSQ PEKFKNVREF VCSGGVSSNQ RLRTKLETEL GTLNSTSFFN
     351  FYYPPMDLCS DNSIMIGWAG IEIWESLRLV SDLDICPIRQ WPLNDLLSVD
     401  GWRTDQL*                                              

external links for Qri7p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) CYC2008 (complexes) MIPS YeastGFP
YGOB Complexome Pfam domains YeastRC Public Image Repository
YOGY DIP YeastRC Structure Prediction (Seattle)

GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Qri7p
1) Simon M, et al.  (1994) Sequence of the PHO2-POL3 (CDC2) region of chromosome IV of Saccharomyces cerevisiae. Yeast 10(12):1653-6
2) Saiz JE, et al.  (1996) The sequence of a 20.3 kb DNA fragment from the left arm of Saccharomyces cerevisiae chromosome IV contains the KIN28, MSS2, PHO2, POL3 and DUN1 genes, and six new open reading frames. Yeast 12(10B Suppl):1077-84
3) Jonson L, et al.  (2004) Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae. Eur J Biochem 271(23-24):4788-97
4) Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
5) Oberto J, et al.  (2009) Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance. Nucleic Acids Res 37(16):5343-52
6) Srinivasan M, et al.  (2011) The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A. EMBO J 30(5):873-81
7) Wan LC, et al.  (2013) Reconstitution and characterization of eukaryotic N6-threonylcarbamoylation of tRNA using a minimal enzyme system. Nucleic Acids Res 41(12):6332-46
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41