QRI7/YDL104C Protein Information

Standard Name	Qri7p 1
Systematic Name	Ydl104cp
ORF Classification	Verified
Description	Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification (2, 3, 4, 5, 6)

Experimental Data
Molecules/cell	1400 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	407
Molecular Weight (Da)	45,543
Isoelectric Point (pI)	7.67

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Qri7p (InterPro)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000132E6F \| P43122 MIPS: YDL104C NCBI: 1172805 \| 1199545 \| 1431146 \| 6320099 \| 683704 \| NP_010179.1 GenBank/EMBL/DDBJ: DAA11756.1 \| X79380 \| X95644 \| Z74152
External Classifications	EC: 3.4.24.- [Metalloendopeptidases]

Amino Acid Sequence (or in FASTA format)
1 MISIKGTGRF LLDNYRIWQR RAFNRPIQLR KGYKVLAIET SCDDTCVSVL 51 DRFSKSAAPN VLANLKDTLD SIDEGGIIPT KAHIHHQARI GPLTERALIE 101 SNAREGIDLI CVTRGPGMPG SLSGGLDFAK GLAVAWNKPL IGVHHMLGHL 151 LIPRMGTNGK VPQFPFVSLL VSGGHTTFVL SRAIDDHEIL CDTIDIAVGD 201 SLDKCGRELG FKGTMIAREM EKFINQDIND QDFALKLEMP SPLKNSASKR 251 NMLSFSFSAF ITALRTNLTK LGKTEIQELP EREIRSIAYQ VQESVFDHII 301 NKLKHVLKSQ PEKFKNVREF VCSGGVSSNQ RLRTKLETEL GTLNSTSFFN 351 FYYPPMDLCS DNSIMIGWAG IEIWESLRLV SDLDICPIRQ WPLNDLLSVD 401 GWRTDQL*

Amino Acid Sequence (or in FASTA format)

       1  MISIKGTGRF LLDNYRIWQR RAFNRPIQLR KGYKVLAIET SCDDTCVSVL
      51  DRFSKSAAPN VLANLKDTLD SIDEGGIIPT KAHIHHQARI GPLTERALIE
     101  SNAREGIDLI CVTRGPGMPG SLSGGLDFAK GLAVAWNKPL IGVHHMLGHL
     151  LIPRMGTNGK VPQFPFVSLL VSGGHTTFVL SRAIDDHEIL CDTIDIAVGD
     201  SLDKCGRELG FKGTMIAREM EKFINQDIND QDFALKLEMP SPLKNSASKR
     251  NMLSFSFSAF ITALRTNLTK LGKTEIQELP EREIRSIAYQ VQESVFDHII
     301  NKLKHVLKSQ PEKFKNVREF VCSGGVSSNQ RLRTKLETEL GTLNSTSFFN
     351  FYYPPMDLCS DNSIMIGWAG IEIWESLRLV SDLDICPIRQ WPLNDLLSVD
     401  GWRTDQL*

external links for Qri7p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Qri7p

1)	Simon M, et al. (1994) Sequence of the PHO2-POL3 (CDC2) region of chromosome IV of Saccharomyces cerevisiae. Yeast 10(12):1653-6
2)	Saiz JE, et al. (1996) The sequence of a 20.3 kb DNA fragment from the left arm of Saccharomyces cerevisiae chromosome IV contains the KIN28, MSS2, PHO2, POL3 and DUN1 genes, and six new open reading frames. Yeast 12(10B Suppl):1077-84
3)	Jonson L, et al. (2004) Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae. Eur J Biochem 271(23-24):4788-97
4)	Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
5)	Oberto J, et al. (2009) Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance. Nucleic Acids Res 37(16):5343-52
6)	Srinivasan M, et al. (2011) The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A. EMBO J 30(5):873-81
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41