DUN1/YDL101C Protein Information Help

Standard Name Dun1p 1
Systematic Name Ydl101cp
ORF Classification Verified
Description Cell-cycle checkpoint serine-threonine kinase; required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; Mec1p and Dun1p function in same pathway to regulate both dNTP pools and telomere length; also regulates postreplicative DNA repair (2, 3, 4, 5, 6)
Name Description DNA-damage UNinducible 7
Experimental Data
Molecules/cell 3480 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 513
Molecular Weight (Da) 58,632
Isoelectric Point (pI) 7.88

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Dun1p (InterPro)
Physical Interactions There are 71 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000129959 | P39009
MIPS: YDL101C
NCBI: 1199548 | 1431140 | 192987132 | 192987134 | 435617 | 6320102 | 729375 | NP_010182.1
GenBank/EMBL/DDBJ: DAA11759.1 | L25548 | X95644 | Z74149
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSLSTKREHS GDVTDSSFKR QQRSNKPSSE YTCLGHLVNL IPGKEQKVEI
      51  TNRNVTTIGR SRSCDVILSE PDISTFHAEF HLLQMDVDNF QRNLINVIDK
     101  SRNGTFINGN RLVKKDYILK NGDRIVFGKS CSFLFKYASS SSTDIENDDE
     151  KVSSESRSYK NDDEVFKKPQ ISATSSQNAT TSAAIRKLNK TRPVSFFDKY
     201  LLGKELGAGH YALVKEAKNK KTGQQVAVKI FHAQQNDDQK KNKQFREETN
     251  ILMRVQHPNI VNLLDSFVEP ISKSQIQKYL VLEKIDDGEL FERIVRKTCL
     301  RQDESKALFK QLLTGLKYLH EQNIIHRDIK PENILLNITR RENPSQVQLG
     351  PWDEDEIDIQ VKIADFGLAK FTGEMQFTNT LCGTPSYVAP EVLTKKGYTS
     401  KVDLWSAGVI LYVCLCGFPP FSDQLGPPSL KEQILQAKYA FYSPYWDKID
     451  DSVLHLISNL LVLNPDERYN IDEALNHPWF NDIQQQSSVS LELQRLQITD
     501  NKIPKTYSEL SCL*

external links for Dun1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Dun1p
1) Elledge, S.  (1993) Personal Communication, Mortimer Map Edition 12
2) Blanchard H, et al.  (2001) Crystallization and preliminary X-ray diffraction studies of FHA domains of Dun1 and Rad53 protein kinases. Acta Crystallogr D Biol Crystallogr 57(Pt 3):459-61
3) Zhao X and Rothstein R  (2002) The Dun1 checkpoint kinase phosphorylates and regulates the ribonucleotide reductase inhibitor Sml1. Proc Natl Acad Sci U S A 99(6):3746-51
4) Hammet A, et al.  (2002) Posttranscriptional regulation of the RAD5 DNA repair gene by the Dun1 kinase and the Pan2-Pan3 poly(A)-nuclease complex contributes to survival of replication blocks. J Biol Chem 277(25):22469-74
5) Bashkirov VI, et al.  (2003) Direct kinase-to-kinase signaling mediated by the FHA phosphoprotein recognition domain of the Dun1 DNA damage checkpoint kinase. Mol Cell Biol 23(4):1441-52
6) Gupta A, et al.  (2013) Telomere Length Homeostasis Responds to Changes in Intracellular dNTP Pools. Genetics 193(4):1095-105
7) Zhou Z and Elledge SJ  (1993) DUN1 encodes a protein kinase that controls the DNA damage response in yeast. Cell 75(6):1119-27
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41