PMT1/YDL095W Protein Information Help

Standard Name Pmt1p 1
Systematic Name Ydl095wp
ORF Classification Verified
Description Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen (1, 2, 3, 4, 5)
Name Description Protein O-MannosylTransferase 1
Experimental Data
Molecules/cell 41500 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 817
Molecular Weight (Da) 92,675
Isoelectric Point (pI) 6.68

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pmt1p (InterPro)
Transmembrane Domains There are 9 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 55 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000011DD21 | P33775
MIPS: YDL095W
NCBI: 1199553 | 1431130 | 388211 | 461956 | 6320107 | NP_010188.1 | NM_001180154.1
GenBank/EMBL/DDBJ: DAA11764.1 | L19169 | X95644 | Z74144
External Classifications EC: 2.4.1.109 [Dolichyl-phosphate-mannose-protein mannosyltransferase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSEEKTYKRV EQDDPVPELD IKQGPVRPFI VTDPSAELAS LRTMVTLKEK
      51  LLVACLAVFT AVIRLHGLAW PDSVVFDEVH FGGFASQYIR GTYFMDVHPP
     101  LAKMLYAGVA SLGGFQGDFD FENIGDSFPS TTPYVLMRFF SASLGALTVI
     151  LMYMTLRYSG VRMWVALMSA ICFAVENSYV TISRYILLDA PLMFFIAAAV
     201  YSFKKYEMYP ANSLNAYKSL LATGIALGMA SSSKWVGLFT VTWVGLLCIW
     251  RLWFMIGDLT KSSKSIFKVA FAKLAFLLGV PFALYLVFFY IHFQSLTLDG
     301  DGASFFSPEF RSTLKNNKIP QNVVADVGIG SIISLRHLST MGGYLHSHSH
     351  NYPAGSEQQQ STLYPHMDAN NDWLLELYNA PGESLTTFQN LTDGTKVRLF
     401  HTVTRCRLHS HDHKPPVSES SDWQKEVSCY GYSGFDGDAN DDWVVEIDKK
     451  NSAPGVAQER VIALDTKFRL RHAMTGCYLF SHEVKLPAWG FEQQEVTCAS
     501  SGRHDLTLWY VENNSNPLLP EDTKRISYKP ASFISKFIES HKKMWHINKN
     551  LVEPHVYESQ PTSWPFLLRG ISYWGENNRN VYLLGNAIVW WAVTAFIGIF
     601  GLIVITELFS WQLGKPILKD SKVVNFHVQV IHYLLGFAVH YAPSFLMQRQ
     651  MFLHHYLPAY YFGILALGHA LDIIVSYVFR SKRQMGYAVV ITFLAASVYF
     701  FKSFSPIIYG TPWTQELCQK SQWLSGWDYN CNTYFSSLEE YKNQTLTKRE
     751  SQPAATSTVE EITIEGDGPS YEDLMNEDGK KIFKDTEGNE LDPEVVKKML
     801  EEEGANILKV EKRAVLE*

external links for Pmt1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Pmt1p
1) Strahl-Bolsinger S, et al.  (1993) PMT1, the gene for a key enzyme of protein O-glycosylation in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 90(17):8164-8
2) Strahl-Bolsinger S and Scheinost A  (1999) Transmembrane topology of pmt1p, a member of an evolutionarily conserved family of protein O-mannosyltransferases. J Biol Chem 274(13):9068-75
3) Gentzsch M and Tanner W  (1996) The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital. EMBO J 15(21):5752-9
4) Gentzsch M, et al.  (1995) Protein O-glycosylation in Saccharomyces cerevisiae: the protein O-mannosyltransferases Pmt1p and Pmt2p function as heterodimer. FEBS Lett 377(2):128-30
5) Goder V and Melero A  (2011) Protein O-mannosyltransferases participate in ER protein quality control. J Cell Sci 124(Pt 1):144-53
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41