RAM1/YDL090C Protein Information Help

Standard Name Ram1p
Systematic Name Ydl090cp
Alias Dpr1p , Fus8p , Scg2p , Sgp2p , Ste16p 1
ORF Classification Verified
Description Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit (2, 3, 4, 5, 6)
Name Description RAS protein and A-factor Maturation 7
Experimental Data
Molecules/cell 3910 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 431
Molecular Weight (Da) 48,189
Isoelectric Point (pI) 6.35

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ram1p (InterPro)
Physical Interactions There are 8 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052F5D | P22007
MIPS: YDL090C
NCBI: 1199557 | 1431120 | 171420 | 2506787 | 51013393 | 6320113 | NP_010193.1
GenBank/EMBL/DDBJ: DAA11768.1 | AY692971 | M22753 | X95644 | Z74138
External Classifications EC: 2.5.1.58 [Protein farnesyltransferase]
Amino Acid Sequence (or in FASTA format)
 
       1  MRQRVGRSIA RAKFINTALL GRKRPVMERV VDIAHVDSSK AIQPLMKELE
      51  TDTTEARYKV LQSVLEIYDD EKNIEPALTK EFHKMYLDVA FEISLPPQMT
     101  ALDASQPWML YWIANSLKVM DRDWLSDDTK RKIVDKLFTI SPSGGPFGGG
     151  PGQLSHLAST YAAINALSLC DNIDGCWDRI DRKGIYQWLI SLKEPNGGFK
     201  TCLEVGEVDT RGIYCALSIA TLLNILTEEL TEGVLNYLKN CQNYEGGFGS
     251  CPHVDEAHGG YTFCATASLA ILRSMDQINV EKLLEWSSAR QLQEERGFCG
     301  RSNKLVDGCY SFWVGGSAAI LEAFGYGQCF NKHALRDYIL YCCQEKEQPG
     351  LRDKPGAHSD FYHTNYCLLG LAVAESSYSC TPNDSPHNIK CTPDRLIGSS
     401  KLTDVNPVYG LPIENVRKII HYFKSNLSSP S*

external links for Ram1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Ram1p
1) Wilson KL and Herskowitz I  (1987) STE16, a new gene required for pheromone production by a cells of Saccharomyces cerevisiae. Genetics 115(3):441-9
2) Trueblood CE, et al.  (1997) Substrate specificity determinants in the farnesyltransferase beta-subunit. Proc Natl Acad Sci U S A 94(20):10774-9
3) Gomez R, et al.  (1993) Purified yeast protein farnesyltransferase is structurally and functionally similar to its mammalian counterpart. Biochem J 289 ( Pt 1)():25-31
4) He B, et al.  (1991) RAM2, an essential gene of yeast, and RAM1 encode the two polypeptide components of the farnesyltransferase that prenylates a-factor and Ras proteins. Proc Natl Acad Sci U S A 88(24):11373-7
5) Kohl NE, et al.  (1991) Structural homology among mammalian and Saccharomyces cerevisiae isoprenyl-protein transferases. J Biol Chem 266(28):18884-8
6) Kurth DD, et al.  (1997) Functional consequence of mutating conserved residues of the yeast farnesyl-protein transferase beta-subunit Ram1(Dpr1). Biochemistry 36(50):15932-9
7) Powers S, et al.  (1986) RAM, a gene of yeast required for a functional modification of RAS proteins and for production of mating pheromone a-factor. Cell 47(3):413-22
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41