MRK1/YDL079C Protein Information

Standard Name	Mrk1p
Systematic Name	Ydl079cp
ORF Classification	Verified
Description	Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation; MRK1 has a paralog, RIM11, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	Mds1p Related Kinase 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	501
Molecular Weight (Da)	57,819
Isoelectric Point (pI)	8.04

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mrk1p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000069B27 \| P50873 MIPS: YDL079C NCBI: 1431098 \| 51704298 \| 6320124 \| 726282 \| NP_010204.1 GenBank/EMBL/DDBJ: DAA11780.1 \| U22348 \| Z74127
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MTDVLRSLVR KISFNNSDNL QLKHKTSIQS NTALEKKKRK PDTIKKVSDV 51 QVHHTVPNFN NSSEYINDIE NLIISKLIDG GKEGIAVDHI EHANISDSKT 101 DGKVANKHEN ISSKLSKEKV EKMINFDYRY IKTKERTIHK RVYKHDRKTD 151 VDRKNHGGTI DISYPTTEVV GHGSFGVVVT TVIIETNQKV AIKKVLQDRR 201 YKNRELETMK MLCHPNTVGL QYYFYEKDEE DEVYLNLVLD YMPQSLYQRL 251 RHFVNLKMQM PRVEIKFYAY QLFKALNYLH NVPRICHRDI KPQNLLVDPT 301 TFSFKICDFG SAKCLKPDQP NVSYICSRYY RAPELMFGAT NYSNQVDVWS 351 SACVIAELLL GKPLFSGESG IDQLVEIIKI MGIPTKDEIS GMNPNYEDHV 401 FPNIKPITLA EIFKAEDPDT LDLLTKTLKY HPCERLVPLQ CLLSSYFDET 451 KRCDTDTYVK AQNLRIFDFD VETELGHVPL VERPAIEERL KHFVSAPSSS 501 L*

Amino Acid Sequence (or in FASTA format)

       1  MTDVLRSLVR KISFNNSDNL QLKHKTSIQS NTALEKKKRK PDTIKKVSDV
      51  QVHHTVPNFN NSSEYINDIE NLIISKLIDG GKEGIAVDHI EHANISDSKT
     101  DGKVANKHEN ISSKLSKEKV EKMINFDYRY IKTKERTIHK RVYKHDRKTD
     151  VDRKNHGGTI DISYPTTEVV GHGSFGVVVT TVIIETNQKV AIKKVLQDRR
     201  YKNRELETMK MLCHPNTVGL QYYFYEKDEE DEVYLNLVLD YMPQSLYQRL
     251  RHFVNLKMQM PRVEIKFYAY QLFKALNYLH NVPRICHRDI KPQNLLVDPT
     301  TFSFKICDFG SAKCLKPDQP NVSYICSRYY RAPELMFGAT NYSNQVDVWS
     351  SACVIAELLL GKPLFSGESG IDQLVEIIKI MGIPTKDEIS GMNPNYEDHV
     401  FPNIKPITLA EIFKAEDPDT LDLLTKTLKY HPCERLVPLQ CLLSSYFDET
     451  KRCDTDTYVK AQNLRIFDFD VETELGHVPL VERPAIEERL KHFVSAPSSS
     501  L*

external links for Mrk1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Mrk1p

1)	Hardy TA, et al. (1995) Novel Saccharomyces cerevisiae gene, MRK1, encoding a putative protein kinase with similarity to mammalian glycogen synthase kinase-3 and Drosophila Zeste-White3/Shaggy. Biochem Biophys Res Commun 208(2):728-34
2)	Andoh T, et al. (2000) Yeast glycogen synthase kinase 3 is involved in protein degradation in cooperation with Bul1, Bul2, and Rsp5. Mol Cell Biol 20(18):6712-20
3)	Hirata Y, et al. (2003) Yeast glycogen synthase kinase-3 activates Msn2p-dependent transcription of stress responsive genes. Mol Biol Cell 14(1):302-12
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61