USO1/YDL058W Protein Information

Standard Name	Uso1p 1
Systematic Name	Ydl058wp
Alias	Int1p
ORF Classification	Verified
Description	Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex (1, 2, 3, 4)
Name Description	yUSOu - transport in Japanese 1

Experimental Data
Molecules/cell	2330 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,790
Molecular Weight (Da)	206,450
Isoelectric Point (pI)	4.7

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Uso1p (InterPro)
Physical Interactions	There are 23 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FC0 \| P25386 MIPS: YDL058W NCBI: 1297348 \| 1431059 \| 1903280 \| 4778 \| 56757595 \| 6320145 \| 677198 \| NP_010225.1 GenBank/EMBL/DDBJ: DAA11798.1 \| L03188 \| U53668 \| X54378 \| Z74105 \| Z74106

Amino Acid Sequence (or in FASTA format)
1 MDIIQGLIQQ PKIQSVDETI PTLCDRVENS TLISDRRSAV LGLKAFSRQY 51 RESVIASGLK PLLNTLKRDY MDEDSVKAIL ETILILFIRG DGHDDLTRGW 101 ISQQSRLQNG KYPSPLVMKQ EKEQVDQFSL WIADALTQSE DLIHLLVEFW 151 EIDNFHIRLY TIQLLEAVMA TRPLKARSAL ISLPTSISTM VSLLDDMHEP 201 IRDEAILLLM AVVNDSPHVQ KLVAFENIFE RLFSIIEEEG GLRGSLVVND 251 CLSLINNILK YNTSNQTLFL ETGNLPKLAH LLSEPISQDE VFFWNDQRIV 301 NINTALDIVS LTVEPGNTVT TKHQNALLDS SVLMVVLRLA FFHNIPKKVR 351 PVALLTAANM VRSNEHAQLE FSKIDVPYFD PSLPVNSTAN GGPIKLIPVV 401 SILINWMLYA NSVHTFDTRV ACSRLLKAYF MDNFDLQRDF LLKQVQLCNN 451 STNNVGDNAK ENGGSNKSDK ESDSDKDTDG KDGTEYEGSF KANLFEVLLN 501 YDAELNLNPF KLFFTTDIFM FFFQQDHKYS EELREITRNV TTGNDLEDEE 551 PLKAIQTISE LLTTSLTAAD IRIPISYLTF LIYWLFGDFK ATNDFLSDKS 601 VIKSLLSFSY QIQDEDVTIK CLVTMLLGVA YEFSSKESPF PRKEYFEFIT 651 KTLGKDNYAS RIKQFKKDSY FSKVDMNEDS ILTPELDETG LPKVYFSTYF 701 IQLFNENIYR IRTALSHDPD EEPINKISFE EVEKLQRQCT KLKGEITSLQ 751 TETESTHENL TEKLIALTNE HKELDEKYQI LNSSHSSLKE NFSILETELK 801 NVRDSLDEMT QLRDVLETKD KENQTALLEY KSTIHKQEDS IKTLEKGLET 851 ILSQKKKAED GINKMGKDLF ALSREMQAVE ENCKNLQKEK DKSNVNHQKE 901 TKSLKEDIAA KITEIKAINE NLEEMKIQCN NLSKEKEHIS KELVEYKSRF 951 QSHDNLVAKL TEKLKSLANN YKDMQAENES LIKAVEESKN ESSIQLSNLQ 1001 NKIDSMSQEK ENFQIERGSI EKNIEQLKKT ISDLEQTKEE IISKSDSSKD 1051 EYESQISLLK EKLETATTAN DENVNKISEL TKTREELEAE LAAYKNLKNE 1101 LETKLETSEK ALKEVKENEE HLKEEKIQLE KEATETKQQL NSLRANLESL 1151 EKEHEDLAAQ LKKYEEQIAN KERQYNEEIS QLNDEITSTQ QENESIKKKN 1201 DELEGEVKAM KSTSEEQSNL KKSEIDALNL QIKELKKKNE TNEASLLESI 1251 KSVESETVKI KELQDECNFK EKEVSELEDK LKASEDKNSK YLELQKESEK 1301 IKEELDAKTT ELKIQLEKIT NLSKAKEKSE SELSRLKKTS SEERKNAEEQ 1351 LEKLKNEIQI KNQAFEKERK LLNEGSSTIT QEYSEKINTL EDELIRLQNE 1401 NELKAKEIDN TRSELEKVSL SNDELLEEKQ NTIKSLQDEI LSYKDKITRN 1451 DEKLLSIERD NKRDLESLKE QLRAAQESKA KVEEGLKKLE EESSKEKAEL 1501 EKSKEMMKKL ESTIESNETE LKSSMETIRK SDEKLEQSKK SAEEDIKNLQ 1551 HEKSDLISRI NESEKDIEEL KSKLRIEAKS GSELETVKQE LNNAQEKIRI 1601 NAEENTVLKS KLEDIERELK DKQAEIKSNQ EEKELLTSRL KELEQELDST 1651 QQKAQKSEEE RRAEVRKFQV EKSQLDEKAM LLETKYNDLV NKEQAWKRDE 1701 DTVKKTTDSQ RQEIEKLAKE LDNLKAENSK LKEANEDRSE IDDLMLLVTD 1751 LDEKNAKYRS KLKDLGVEIS SDEEDDEEDD EEDEEEGQVA *

Amino Acid Sequence (or in FASTA format)

       1  MDIIQGLIQQ PKIQSVDETI PTLCDRVENS TLISDRRSAV LGLKAFSRQY
      51  RESVIASGLK PLLNTLKRDY MDEDSVKAIL ETILILFIRG DGHDDLTRGW
     101  ISQQSRLQNG KYPSPLVMKQ EKEQVDQFSL WIADALTQSE DLIHLLVEFW
     151  EIDNFHIRLY TIQLLEAVMA TRPLKARSAL ISLPTSISTM VSLLDDMHEP
     201  IRDEAILLLM AVVNDSPHVQ KLVAFENIFE RLFSIIEEEG GLRGSLVVND
     251  CLSLINNILK YNTSNQTLFL ETGNLPKLAH LLSEPISQDE VFFWNDQRIV
     301  NINTALDIVS LTVEPGNTVT TKHQNALLDS SVLMVVLRLA FFHNIPKKVR
     351  PVALLTAANM VRSNEHAQLE FSKIDVPYFD PSLPVNSTAN GGPIKLIPVV
     401  SILINWMLYA NSVHTFDTRV ACSRLLKAYF MDNFDLQRDF LLKQVQLCNN
     451  STNNVGDNAK ENGGSNKSDK ESDSDKDTDG KDGTEYEGSF KANLFEVLLN
     501  YDAELNLNPF KLFFTTDIFM FFFQQDHKYS EELREITRNV TTGNDLEDEE
     551  PLKAIQTISE LLTTSLTAAD IRIPISYLTF LIYWLFGDFK ATNDFLSDKS
     601  VIKSLLSFSY QIQDEDVTIK CLVTMLLGVA YEFSSKESPF PRKEYFEFIT
     651  KTLGKDNYAS RIKQFKKDSY FSKVDMNEDS ILTPELDETG LPKVYFSTYF
     701  IQLFNENIYR IRTALSHDPD EEPINKISFE EVEKLQRQCT KLKGEITSLQ
     751  TETESTHENL TEKLIALTNE HKELDEKYQI LNSSHSSLKE NFSILETELK
     801  NVRDSLDEMT QLRDVLETKD KENQTALLEY KSTIHKQEDS IKTLEKGLET
     851  ILSQKKKAED GINKMGKDLF ALSREMQAVE ENCKNLQKEK DKSNVNHQKE
     901  TKSLKEDIAA KITEIKAINE NLEEMKIQCN NLSKEKEHIS KELVEYKSRF
     951  QSHDNLVAKL TEKLKSLANN YKDMQAENES LIKAVEESKN ESSIQLSNLQ
    1001  NKIDSMSQEK ENFQIERGSI EKNIEQLKKT ISDLEQTKEE IISKSDSSKD
    1051  EYESQISLLK EKLETATTAN DENVNKISEL TKTREELEAE LAAYKNLKNE
    1101  LETKLETSEK ALKEVKENEE HLKEEKIQLE KEATETKQQL NSLRANLESL
    1151  EKEHEDLAAQ LKKYEEQIAN KERQYNEEIS QLNDEITSTQ QENESIKKKN
    1201  DELEGEVKAM KSTSEEQSNL KKSEIDALNL QIKELKKKNE TNEASLLESI
    1251  KSVESETVKI KELQDECNFK EKEVSELEDK LKASEDKNSK YLELQKESEK
    1301  IKEELDAKTT ELKIQLEKIT NLSKAKEKSE SELSRLKKTS SEERKNAEEQ
    1351  LEKLKNEIQI KNQAFEKERK LLNEGSSTIT QEYSEKINTL EDELIRLQNE
    1401  NELKAKEIDN TRSELEKVSL SNDELLEEKQ NTIKSLQDEI LSYKDKITRN
    1451  DEKLLSIERD NKRDLESLKE QLRAAQESKA KVEEGLKKLE EESSKEKAEL
    1501  EKSKEMMKKL ESTIESNETE LKSSMETIRK SDEKLEQSKK SAEEDIKNLQ
    1551  HEKSDLISRI NESEKDIEEL KSKLRIEAKS GSELETVKQE LNNAQEKIRI
    1601  NAEENTVLKS KLEDIERELK DKQAEIKSNQ EEKELLTSRL KELEQELDST
    1651  QQKAQKSEEE RRAEVRKFQV EKSQLDEKAM LLETKYNDLV NKEQAWKRDE
    1701  DTVKKTTDSQ RQEIEKLAKE LDNLKAENSK LKEANEDRSE IDDLMLLVTD
    1751  LDEKNAKYRS KLKDLGVEIS SDEEDDEEDD EEDEEEGQVA *

external links for Uso1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Uso1p

1)	Nakajima H, et al. (1991) A cytoskeleton-related gene, uso1, is required for intracellular protein transport in Saccharomyces cerevisiae. J Cell Biol 113(2):245-60
2)	Cao X, et al. (1998) Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins. EMBO J 17(8):2156-65
3)	Sapperstein SK, et al. (1996) Assembly of the ER to Golgi SNARE complex requires Uso1p. J Cell Biol 132(5):755-67
4)	Noda Y, et al. (2007) Specific membrane recruitment of Uso1 protein, the essential endoplasmic reticulum-to-Golgi tethering factor in yeast vesicular transport. J Cell Biochem 101(3):686-94
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41