PSA1/YDL055C Protein Information

Standard Name	Psa1p 1
Systematic Name	Ydl055cp
Alias	Mpg1p , Vig9p , Srb1p
ORF Classification	Verified
Description	GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure (2, 3)

Experimental Data
Molecules/cell	97100 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	361
Molecular Weight (Da)	39,566
Isoelectric Point (pI)	6.29

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Psa1p (InterPro)
Physical Interactions	There are 115 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FAB \| P41940 MIPS: YDL055C NCBI: 1292898 \| 1431053 \| 1709086 \| 6320148 \| 894204 \| NP_010228.1 GenBank/EMBL/DDBJ: DAA11801.1 \| U19608 \| U24437 \| Z74103
External Classifications	EC: 2.7.7.13 [Mannose-1-phosphate guanylyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MKGLILVGGY GTRLRPLTLT VPKPLVEFGN RPMILHQIEA LANAGVTDIV 51 LAVNYRPEVM VETLKKYEKE YGVNITFSVE TEPLGTAGPL KLAEDVLKKD 101 NSPFFVLNSD VICEYPFKEL ADFHKAHGGK GTIVATKVDE PSKYGVIVHD 151 IATPNLIDRF VEKPKEFVGN RINAGLYILN PEVIDLIEMK PTSIEKETFP 201 ILVEEKQLYS FDLEGFWMDV GQPKDFLSGT VLYLNSLAKR QPKKLATGAN 251 IVGNALIDPT AKISSTAKIG PDVVIGPNVT IGDGVRITRS VVLCNSTIKN 301 HSLVKSTIVG WNSTVGQWCR LEGVTVLGDD VEVKDEIYIN GGKVLPHKSI 351 SDNVPKEAII M*

Amino Acid Sequence (or in FASTA format)

       1  MKGLILVGGY GTRLRPLTLT VPKPLVEFGN RPMILHQIEA LANAGVTDIV
      51  LAVNYRPEVM VETLKKYEKE YGVNITFSVE TEPLGTAGPL KLAEDVLKKD
     101  NSPFFVLNSD VICEYPFKEL ADFHKAHGGK GTIVATKVDE PSKYGVIVHD
     151  IATPNLIDRF VEKPKEFVGN RINAGLYILN PEVIDLIEMK PTSIEKETFP
     201  ILVEEKQLYS FDLEGFWMDV GQPKDFLSGT VLYLNSLAKR QPKKLATGAN
     251  IVGNALIDPT AKISSTAKIG PDVVIGPNVT IGDGVRITRS VVLCNSTIKN
     301  HSLVKSTIVG WNSTVGQWCR LEGVTVLGDD VEVKDEIYIN GGKVLPHKSI
     351  SDNVPKEAII M*

external links for Psa1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Psa1p

1)	Schultz, J. and G.F. Sprague (1995) Isolation of the gene encoding mannose-1-phosphate guanyltransferase from yeast. Unpublished
2)	Hashimoto H, et al. (1997) Saccharomyces cerevisiae VIG9 encodes GDP-mannose pyrophosphorylase, which is essential for protein glycosylation. J Biol Chem 272(26):16308-14
3)	Yoda K, et al. (2000) Defect in cell wall integrity of the yeast saccharomyces cerevisiae caused by a mutation of the GDP-mannose pyrophosphorylase gene VIG9. Biosci Biotechnol Biochem 64(9):1937-41
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41