MTF2/YDL044C Protein Information

Standard Name	Mtf2p
Systematic Name	Ydl044cp
Alias	Nam1p
ORF Classification	Verified
Description	Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription (1, 2, 3)
Name Description	Mitochondrial Transcription Factor 4, 5

Experimental Data
Molecules/cell	2060 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	440
Molecular Weight (Da)	51,192
Isoelectric Point (pI)	6.72

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mtf2p (InterPro)
Physical Interactions	There are 15 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012FD3D \| P10849 MIPS: YDL044C NCBI: 127823 \| 1279672 \| 1431032 \| 2951 \| 4010 \| 6320160 \| NP_010240.1 GenBank/EMBL/DDBJ: DAA11812.1 \| X14719 \| X51665 \| Z71781 \| Z74092

Amino Acid Sequence (or in FASTA format)
1 MIRTSSILKN CNYRYIHCIH RCLLNEANLK DRKTHNVERV SNEKTFEQAL 51 EEERKVFGEL FEAGARVENM RHTNASKIID KYYNGLQDNS EGTSVKKEKI 101 VFNHSQRAQR KLPNKDHEFL KETAGNDYVY ERAEPSAIST KTISEQTRTL 151 LEKIFDEDNS INKSNRELLN LNLRKGSGME ALRQPVAHSN VKFSEEVMQE 201 IGNKIRYQTT LDQVLEPHID YLREAVKSDY DLLRYLKQSL DIYKKRNKDL 251 ELKMNAESSN IFEDIRSACI NKPAELPKPL AMTLPYIIVK SLRLGDFDFP 301 ADRKYTLISY VYNECKNNMD ASLYLTICNV DFYNLLVQLL WENFQEIRYL 351 RRVVTEMSVN GVIGNIETVD ILDKIVKEMR SLNEDVFLEA GEQLSADEEV 401 SSSANKIVNV GVLWNKDTNN DLLIVENYLK SLKKNLTRDR *

Amino Acid Sequence (or in FASTA format)

       1  MIRTSSILKN CNYRYIHCIH RCLLNEANLK DRKTHNVERV SNEKTFEQAL
      51  EEERKVFGEL FEAGARVENM RHTNASKIID KYYNGLQDNS EGTSVKKEKI
     101  VFNHSQRAQR KLPNKDHEFL KETAGNDYVY ERAEPSAIST KTISEQTRTL
     151  LEKIFDEDNS INKSNRELLN LNLRKGSGME ALRQPVAHSN VKFSEEVMQE
     201  IGNKIRYQTT LDQVLEPHID YLREAVKSDY DLLRYLKQSL DIYKKRNKDL
     251  ELKMNAESSN IFEDIRSACI NKPAELPKPL AMTLPYIIVK SLRLGDFDFP
     301  ADRKYTLISY VYNECKNNMD ASLYLTICNV DFYNLLVQLL WENFQEIRYL
     351  RRVVTEMSVN GVIGNIETVD ILDKIVKEMR SLNEDVFLEA GEQLSADEEV
     401  SSSANKIVNV GVLWNKDTNN DLLIVENYLK SLKKNLTRDR *

external links for Mtf2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Mtf2p

1)	Wallis MG, et al. (1994) The NAM1 protein (NAM1p), which is selectively required for cox1, cytb and atp6 transcript processing/stabilisation, is located in the yeast mitochondrial matrix. Eur J Biochem 222(1):27-32
2)	Asher EB, et al. (1989) Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria. Mol Gen Genet 215(3):517-28
3)	Rodeheffer MS, et al. (2001) Nam1p, a protein involved in RNA processing and translation, is coupled to transcription through an interaction with yeast mitochondrial RNA polymerase. J Biol Chem 276(11):8616-22
4)	Lisowsky T (1990) Molecular analysis of the mitochondrial transcription factor mtf2 of Saccharomyces cerevisiae. Mol Gen Genet 220(2):186-90
5)	Lisowsky T and Michaelis G (1989) Mutations in the genes for mitochondrial RNA polymerase and a second mitochondrial transcription factor of Saccharomyces cerevisiae. Mol Gen Genet 219(1-2):125-8
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41