PRP9/YDL030W Protein Information

Standard Name	Prp9p
Systematic Name	Ydl030wp
ORF Classification	Verified
Description	Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex (1, 2)
Name Description	Pre-mRNA Processing 3

Experimental Data
Molecules/cell	3920 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	530
Molecular Weight (Da)	63,029
Isoelectric Point (pI)	6.41

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Prp9p (InterPro)
Physical Interactions	There are 103 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053206 \| P19736 MIPS: YDL030W NCBI: 1279686 \| 130808 \| 1431008 \| 385867774 \| 4241 \| 6320174 \| NP_010254.1 GenBank/EMBL/DDBJ: DAA11822.1 \| X53466 \| Z71781 \| Z74078

Amino Acid Sequence (or in FASTA format)
1 MNLLETRRSL LEEMEIIENA IAERIQRNPE LYYHYIQESS KVFPDTKLPR 51 SSLIAENKIY KFKKVKRKRK QIILQQHEIN IFLRDYQEKQ QTFNKINRPE 101 ETQEDDKDLP NFERKLQQLE KELKNEDENF ELDINSKKDK YALFSSSSDP 151 SRRTNILSDR ARDLDLNEIF TRDEQYGEYM ELEQFHSLWL NVIKRGDCSL 201 LQFLDILELF LDDEKYLLTP PMDRKNDRYM AFLLKLSKYV ETFFFKSYAL 251 LDAAAVENLI KSDFEHSYCR GSLRSEAKGI YCPFCSRWFK TSSVFESHLV 301 GKIHKKNESK RRNFVYSEYK LHRYLKYLND EFSRTRSFVE RKLAFTANER 351 MAEMDILTQK YEAPAYDSTE KEGAEQVDGE QRDGQLQEEH LSGKSFDMPL 401 GPDGLPMPYW LYKLHGLDRE YRCEICSNKV YNGRRTFERH FNEERHIYHL 451 RCLGIEPSSV FKGITKIKEA QELWKNMQGQ SQLTSIAAVP PKPNPSQLKV 501 PTELELEEED EEGNVMSKKV YDELKKQGLV *

Amino Acid Sequence (or in FASTA format)

       1  MNLLETRRSL LEEMEIIENA IAERIQRNPE LYYHYIQESS KVFPDTKLPR
      51  SSLIAENKIY KFKKVKRKRK QIILQQHEIN IFLRDYQEKQ QTFNKINRPE
     101  ETQEDDKDLP NFERKLQQLE KELKNEDENF ELDINSKKDK YALFSSSSDP
     151  SRRTNILSDR ARDLDLNEIF TRDEQYGEYM ELEQFHSLWL NVIKRGDCSL
     201  LQFLDILELF LDDEKYLLTP PMDRKNDRYM AFLLKLSKYV ETFFFKSYAL
     251  LDAAAVENLI KSDFEHSYCR GSLRSEAKGI YCPFCSRWFK TSSVFESHLV
     301  GKIHKKNESK RRNFVYSEYK LHRYLKYLND EFSRTRSFVE RKLAFTANER
     351  MAEMDILTQK YEAPAYDSTE KEGAEQVDGE QRDGQLQEEH LSGKSFDMPL
     401  GPDGLPMPYW LYKLHGLDRE YRCEICSNKV YNGRRTFERH FNEERHIYHL
     451  RCLGIEPSSV FKGITKIKEA QELWKNMQGQ SQLTSIAAVP PKPNPSQLKV
     501  PTELELEEED EEGNVMSKKV YDELKKQGLV *

external links for Prp9p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Prp9p

1)	Hodges PE and Beggs JD (1994) RNA splicing. U2 fulfils a commitment. Curr Biol 4(3):264-7
2)	Legrain P, et al. (1993) Interactions between PRP9 and SPP91 splicing factors identify a protein complex required in prespliceosome assembly. Genes Dev 7(7B):1390-9
3)	Vijayraghavan U, et al. (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41