MPS1/YDL028C Protein Information

Standard Name	Mps1p 1
Systematic Name	Ydl028cp
Alias	Rpk1p , Pac8p 2
ORF Classification	Verified
Description	Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p (3, 4, 5, 6, 7, 8)
Name Description	MonoPolar Spindle 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	764
Molecular Weight (Da)	86,771
Isoelectric Point (pI)	9.36

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mps1p (InterPro)
Physical Interactions	There are 94 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000158E67E \| P54199 MIPS: YDL028C NCBI: 1197058 \| 1279688 \| 1431004 \| 1709092 \| 330443507 \| 341940969 \| NP_010256.2 \| NM_001180087.2 GenBank/EMBL/DDBJ: DAA11824.2 \| L08909 \| Z71781 \| Z74076
External Classifications	EC: 2.7.12.1 [Dual-specificity kinase] EC: 2.7.12.2 [Mitogen-activated protein kinase kinase]

Amino Acid Sequence (or in FASTA format)
1 MSTNSFHDYV DLKSRTNTRQ FSDDEEFTTP PKLSNFGSAL LSHTEKTSAS 51 EILSSHNNDK IANRLEEMDR SSSRSHPPPS MGNLTSGHTS TSSHSTLFGR 101 YLRNNHQTSM TTMNTSDIEI NVGNSLDKSF ERIRNLRQNM KEDITAKYAE 151 RRSKRFLISN RTTKLGPAKR AMTLTNIFDE DVPNSPNQPI NARETVELPL 201 EDSHQTNFKE TKRNTDYDSI DFGDLNPIQY IKKHNLPTSD LPLISQIYFD 251 KQREENRQAA LRKHSSRELL YKSRSSSSSL SSNNLLANKD NSITSNNGSQ 301 PRRKVSTGSS SSKSSIEIRR ALKENIDTSN NSNFNSPIHK IYKGISRNKD 351 SDSEKREVLR NISINANHAD NLLQQENKRL KRSLDDAITN ENINSKNLEV 401 FYHRPAPKPP VTKKVEIVEP AKSASLSNNR NIITVNDSQY EKIELLGRGG 451 SSRVYKVKGS GNRVYALKRV SFDAFDDSSI DGFKGEIELL EKLKDQKRVI 501 QLLDYEMGDG LLYLIMECGD HDLSQILNQR SGMPLDFNFV RFYTKEMLLC 551 IKVVHDAGIV HSDLKPANFV LVKGILKIID FGIANAVPEH TVNIYRETQI 601 GTPNYMAPEA LVAMNYTQNS ENQHEGNKWK VGRPSDMWSC GCIIYQMIYG 651 KPPYGSFQGQ NRLLAIMNPD VKIPFPEHTS NNEKIPKSAI ELMKACLYRN 701 PDKRWTVDKV LSSTFLQPFM ISGSIMEDLI RNAVRYGSEK PHISQDDLND 751 VVDTVLRKFA DYKI*

Amino Acid Sequence (or in FASTA format)

       1  MSTNSFHDYV DLKSRTNTRQ FSDDEEFTTP PKLSNFGSAL LSHTEKTSAS
      51  EILSSHNNDK IANRLEEMDR SSSRSHPPPS MGNLTSGHTS TSSHSTLFGR
     101  YLRNNHQTSM TTMNTSDIEI NVGNSLDKSF ERIRNLRQNM KEDITAKYAE
     151  RRSKRFLISN RTTKLGPAKR AMTLTNIFDE DVPNSPNQPI NARETVELPL
     201  EDSHQTNFKE TKRNTDYDSI DFGDLNPIQY IKKHNLPTSD LPLISQIYFD
     251  KQREENRQAA LRKHSSRELL YKSRSSSSSL SSNNLLANKD NSITSNNGSQ
     301  PRRKVSTGSS SSKSSIEIRR ALKENIDTSN NSNFNSPIHK IYKGISRNKD
     351  SDSEKREVLR NISINANHAD NLLQQENKRL KRSLDDAITN ENINSKNLEV
     401  FYHRPAPKPP VTKKVEIVEP AKSASLSNNR NIITVNDSQY EKIELLGRGG
     451  SSRVYKVKGS GNRVYALKRV SFDAFDDSSI DGFKGEIELL EKLKDQKRVI
     501  QLLDYEMGDG LLYLIMECGD HDLSQILNQR SGMPLDFNFV RFYTKEMLLC
     551  IKVVHDAGIV HSDLKPANFV LVKGILKIID FGIANAVPEH TVNIYRETQI
     601  GTPNYMAPEA LVAMNYTQNS ENQHEGNKWK VGRPSDMWSC GCIIYQMIYG
     651  KPPYGSFQGQ NRLLAIMNPD VKIPFPEHTS NNEKIPKSAI ELMKACLYRN
     701  PDKRWTVDKV LSSTFLQPFM ISGSIMEDLI RNAVRYGSEK PHISQDDLND
     751  VVDTVLRKFA DYKI*

external links for Mps1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Mps1p

1)	Winey M, et al. (1991) MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplication. J Cell Biol 114(4):745-54
2)	Geiser JR, et al. (1997) Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Mol Biol Cell 8(6):1035-50
3)	Lauze E, et al. (1995) Yeast spindle pole body duplication gene MPS1 encodes an essential dual specificity protein kinase. EMBO J 14(8):1655-63
4)	Luca FC and Winey M (1998) MOB1, an essential yeast gene required for completion of mitosis and maintenance of ploidy. Mol Biol Cell 9(1):29-46
5)	Hardwick KG, et al. (1996) Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption. Science 273(5277):953-6
6)	Friedman DB, et al. (2001) Yeast Mps1p phosphorylates the spindle pole component Spc110p in the N-terminal domain. J Biol Chem 276(21):17958-67
7)	Castillo AR, et al. (2002) The yeast protein kinase Mps1p is required for assembly of the integral spindle pole body component Spc42p. J Cell Biol 156(3):453-65
8)	Malvezzi F, et al. (2013) A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors. EMBO J 32(3):409-23