RTK1/YDL025C Protein Information

Standard Name	Rtk1p 1
Systematic Name	Ydl025cp
ORF Classification	Verified
Description	Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress (1, 2, 3, 4)
Name Description	Ribosome biogenesis and TRNA synthetase-associated Kinase 1

Experimental Data
Molecules/cell	861 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	620
Molecular Weight (Da)	69,615
Isoelectric Point (pI)	8.38

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rtk1p (InterPro)
Physical Interactions	There are 56 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006B845 \| Q12100 MIPS: YDL025C NCBI: 1430999 \| 51012883 \| 6320179 \| 683672 \| 74676523 \| NP_010259.1 GenBank/EMBL/DDBJ: DAA11826.1 \| AY692716 \| Z48432 \| Z74073
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MVKETPLHSS SSTSLSSLFR PTKLKNLSAK IFNGGGNQSY SKTDDVSRSS 51 SRSSKKNTDS DQEDQIKYNK PNDRRSTIGK SPQGNGALSK ESHVVASSTL 101 TGISPTSAKK APIDYSPSRP LPNNHNPVRT GHTVPHLPHS IHNPINYIHQ 151 GSKDAFHHPH PVRSTAHSNI STVSSAKSDT PSSNLSYQAH MHPVEILQKQ 201 IEDKHFMDSQ ASTPGSVELQ HNSSSGSDDT SSRKKKSLRL TRFFKKIHND 251 YHDNHHHHHH HNRGSTPTKP KLNLNTNENI VESNGKALYE TDNPVELLEK 301 YGIPGRKLGE GASGSVSVVE RTDGKLFACK MFRKPHLNNE GTNQSQLANY 351 SKKVTTEFCI GSTLHHENIV ETLDMLTEGD TYLLVMEYAP YDFFNLVMSN 401 LMTQDEVNCY FKQLCHGVNY LHSMGLAHRD LKLDNCVVTK DGILKLIDFG 451 SAVVFQYPYE DTIVKSHGIV GSDPYLAPEL LKQTSYDPRV ADVWSIAIIF 501 YCMVLKRFPW KAPKKSFNSF RLFTEEPEDE DDIVRGPNKI LRLLPRHSRT 551 IIGRMLALEP KQRVLMNDVV KDDWLVSVPS CEVDPTSGDL VEKPKNHKHH 601 LVTEEELNEL TKQHGNKDSN *

Amino Acid Sequence (or in FASTA format)

       1  MVKETPLHSS SSTSLSSLFR PTKLKNLSAK IFNGGGNQSY SKTDDVSRSS
      51  SRSSKKNTDS DQEDQIKYNK PNDRRSTIGK SPQGNGALSK ESHVVASSTL
     101  TGISPTSAKK APIDYSPSRP LPNNHNPVRT GHTVPHLPHS IHNPINYIHQ
     151  GSKDAFHHPH PVRSTAHSNI STVSSAKSDT PSSNLSYQAH MHPVEILQKQ
     201  IEDKHFMDSQ ASTPGSVELQ HNSSSGSDDT SSRKKKSLRL TRFFKKIHND
     251  YHDNHHHHHH HNRGSTPTKP KLNLNTNENI VESNGKALYE TDNPVELLEK
     301  YGIPGRKLGE GASGSVSVVE RTDGKLFACK MFRKPHLNNE GTNQSQLANY
     351  SKKVTTEFCI GSTLHHENIV ETLDMLTEGD TYLLVMEYAP YDFFNLVMSN
     401  LMTQDEVNCY FKQLCHGVNY LHSMGLAHRD LKLDNCVVTK DGILKLIDFG
     451  SAVVFQYPYE DTIVKSHGIV GSDPYLAPEL LKQTSYDPRV ADVWSIAIIF
     501  YCMVLKRFPW KAPKKSFNSF RLFTEEPEDE DDIVRGPNKI LRLLPRHSRT
     551  IIGRMLALEP KQRVLMNDVV KDDWLVSVPS CEVDPTSGDL VEKPKNHKHH
     601  LVTEEELNEL TKQHGNKDSN *

external links for Rtk1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rtk1p

1)	Breitkreutz A, et al. (2010) A global protein kinase and phosphatase interaction network in yeast. Science 328(5981):1043-6
2)	Hunter T and Plowman GD (1997) The protein kinases of budding yeast: six score and more. Trends Biochem Sci 22(1):18-22
3)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
4)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41